The document provides the DNA promoter sequences of six bacterial genes. It asks to identify the Pribnow box sequence located approximately 10 nucleotides before the +1 transcription start site in each sequence, and determine the consensus Pribnow box sequence based on the six examples.
The DNA sequences shown below are from the promoter reglons of six ba.pdf
1. The DNA sequences shown below are from the promoter reglons of six bacterlal genes. In each
case, the last Examine these sequences and identify the Pribnow box sequence at approximately -
10 for each promoter. nucleotide in the sequence is the +1 nucleotide that Enter your answers
separated by commas. initiates transcription. Gene 1 ... TTCCGGCTCGTATGTTGTGTGGB ...
Gene 2 ... CGTCATTTGATATGATGCGCCCCG ... Gene 3 ...
CCACTGGCGGTGATACTGAGCACA ... Gene 4 ... TTTATTGCAGCTTATAATGGTTACA
... Gene 5... TGCTTCTGACTATAATAGACAGGG ... GEE 6...
AAGTAAACACGGTACGATGTACCACA ... Part B Determine the consensus sequence for the
Pribnow box from the sequences above.