bio101

1. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. DNA probe DNA probe array tagged DNA tagged DNA did bind to probe tagged DNA did not bind to probe testing subject's DNA

4. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

6. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

7. Cloning a Human Gene Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. O f r o m a i 1. Restriction enzyme cleaves DNA. 2. DNA ligase seals human gene and plasmid. 3. Host cell takes up recombined plasmid. 4. Gene cloning occurs. bacterium recombinant DNA insulin human cell insulin gene human DNA plasmid

9. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

11. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

12. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

13. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

15. PCR Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. PCR cycles DNA copies first 1 second 2 third 4 old old old strand new new new strand DNA double strand fourth 8 fifth 16 and so forth

16. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

17. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

18. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

20. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

21. DNA Fingerprinting & Paternity Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Mother Child Male 1 Male 2 Base repeat units many few DNA Band patterns Increasing size b. Automated DNA fingerprinting a. Fluorescence units

22. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

24. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

27. Genetically Engineered Bacteria Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. (Both): Courtesy General Electric Research & Development

28. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

30. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

32. Transgenic Mammals Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. human gene for growth hormone human growth hormone microinjection of human gene development within a host goat Transgenic goat produces human growth hormone. a. b. transgenic goat cells with gene for human growth hormone microinjection of transgenic gene into enucleated donor eggs donor of eggs donor of egg enucleated eggs Cloned transgenic goats produce human growth hormone. milk development within host goats milk

35. Gene Therapy Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Brain (gene transfer by injection)* • Huntington disease • Alzheimer disease • Parkinson disease • brain tumors Skin (gene transfer by modified blood cells)** • skin cancer Lungs (gene transfer by aerosol spray)* • cystic fibrosis • hereditary emphysema Liver (gene transfer by modified implants)** • familial hypercholesterolemia Blood (gene transfer by bone marrow transplant)* • sickle-cell disease Muscle (gene transfer by injection)* • Duchenne muscular dystrophy Bone marrow (gene transfer by implantation of modified stem cells)** • SCID • sickle-cell disease Endothelium (blood vessel lining) (gene transfer by implantation of modified implants)** • hemophilia • diabetes mellitus * in vivo ** ex vivo

40. Chromosomal DNA Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. exon exon intron intron exon exon intergenic sequences DNA pre-mRNA RNA introns Gene A Gene B Gene C Gene A mRNA Gene B mRNA Gene C mRNA © Cindy Charles/PhotoEdit 5  3  5  3  3  5 

42. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

43. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

44. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

45. Genomic Differences Between Species Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

48. DNA Microarray Technology Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. DNA probe DNA probe array tagged DNA tagged DNA did bind to probe tagged DNA did not bind to probe testing subject's DNA

49. Animation Please note that due to differing operating systems, some animations will not appear until the presentation is viewed in Presentation Mode (Slide Show view). You may see blank slides in the “Normal” or “Slide Sorter” views. All animations will appear after viewing in Presentation Mode and playing each animation. Most animations will require the latest version of the Flash Player, which is available at http://get.adobe.com/flashplayer.

53. Bioinformatics Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. exon exon intron intron exon exon intergenic sequences DNA pre-mRNA RNA introns Gene A Gene B Gene C Gene A mRNA Gene B mRNA Gene C mRNA 5  3  5  3  3  5 

55. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. DNA probe DNA probe array tagged DNA tagged DNA did bind to probe tagged DNA did not bind to probe testing subject's DNA