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SKILLS 
Biochemistry 
Molecular microbiology 
Astrochemistry/astrobiology 
Geochemistry 
Biogeochemistry 
Geomicrobiology 
Knowledge sharing 
Open access / open science 
Open source hardware
What the Soil Food Web does… 
• Organisms do the work in soil and compost - 
– make nutrients plant-available 
– build soil structure 
– suppress diseases and pests 
• Organisms are fed and supported by plants 
through root exudates and dead plant material 
• Protozoa, nematodes, earthworms and micro-arthropods 
eat bacteria and fungi to release 
nutrients in plant-available forms 
nothing
MICROBES AND SOIL FORMATION 
 3.4.1 Living Organisms 
 ●Bacteria - 
 "A teaspoon of productive 
 soil generally contains 
 between 100 million and 1 
 billion bacteria. That is as 
 much as two cows per acre." 
 - Elaine R. Ingham, NRCS 
 1. Decomposers 
 2. Mutualists - nitrogen-fixers 
 3. Pathogens 
 4. Chemoautotrophs (or 
lithotrophs)
SOIL FERTILITY 
 Bacteria can transform minerals 
 Bacteria can degrade organic matter 
 Many reactions possible 
 CHO  CO2 + H2O 
 SO4SH2S 
 NO3N2 
 NH3 N2 
 NH3  NO3
SOIL BACTERIA 
 Different bacteria work together 
 eg NH3 + NO3 N2 
 CH3  CH4 (need other bacteria 
producing H) 
 Potential for cleaning CH3-Br
RELEVANCE OF ASTROBIOLOGY 
 More bacteria remain to be discovered 
 Astrobiology can provide insight in processes 
at the interface living – non living 
 New leads for processes useful for 
agriculture 
 New leads for environment cleaning
SHARING KNOWLEDGE 
 Agriculture networks of knowledge 
 CIARD, GFAR, AIMS, CGIAR
EXAMPLE OF TOP-DOWN APROACH
SHARING KNOWLEDGE 
What we want: 
bottom-up networks of practitioners, farmers, 
students, citzen scientists 
Use for example iHUB (kenya) 
Use contacts of open science for development 
group 
Use contacts from Open Knowledge global 
network
SHARING KNOWLEDGE 
HOW?
ACCESS TO THE INTERNET FAST GROWING
DEVELOPING WORLD CATCHING UP 
 65% of the people who get onto the internet today 
come from emerging markets (mainly using wireless) 
 Meanwhile, only 24% of the people in emerging 
markets are online. 
 That means that whether the West realizes it or not, 
the internet focus has already shifted, the ripples of 
this are only now being felt.
TRANSFER OF TECHNOLOGY: 
AGRICULTURAL METHODS 
 Easy to follow methods 
 Local production organic fertilizers 
 Local sources 
 Low-cost materials 
 Easy measurements 
 In short RockinSoils
TRANSFER OF TECHNOLOGY: 
MEASURING TOOLS 
 Open Source Hardware OSH 
 Key technology 3D Printing 
Low cost spectrometer, microscope, pH meter, 
conductivity, test tubes, containers and more
OPEN SOURCE HARDWARE 
‘Just as the power of the open-source design paradigm has driven down 
the cost of software to the point that it is accessible to most people, the rise 
of open-source hardware is poised to drive down the cost of doing 
experimental science to expand access to everyone’ 
 You can download designs from 
 Thingiverse or another repository (e.g. grabcad, github.com, 
3Dhacker.com, 
 appropedia.org, etc.)
OPEN-SOURCE COLORIMETER 
 in environmental science, colorimeters are used to monitor the levels of nitrates, 
phosphates,metals and other compounds present in effluent entering the natural 
environment
PUBLIC LABORATORY (HTTP://PUBLICLABORATORY.ORG/HOME) 
The initial versions of the Public Laboratory spectrometers were made by crudely 
taping a small black paper case and a slice of D VD -R to the back of an Android 
phone 
Now using the powerful tool of open-source 3-D printing, you can print 
a superior version of the Public Laboratory spectrometer (v3.0)
OPEN SOURCE SPECTROMETER
3-D PRINTABLE OPEN-SOURCE MICROSCOPE 
WITH SMARTPHONE
3D PRINTED EQUIPMENT 
Laser cut test tube racks 
A) assembled rack 
B) Pattern for cutting 
But also possible 
PCR apparatus
SOLAR CELLS
HACKTERIA.ORG 
There are a growing number of organizations devoted to producing O S H for 
science. One such group is Hackteria.org, which was designed as a 
community 
platform to encourage the collaboration of scientists, hackers and artists to 
combine their expertises
HACKTERIA CONNECTION MAP
OPENSCIDEV MEETING CAPETOWN

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SKILLS AND TOOLS FOR OPEN SCIENCE AND SUSTAINABLE AGRICULTURE

  • 1. SKILLS Biochemistry Molecular microbiology Astrochemistry/astrobiology Geochemistry Biogeochemistry Geomicrobiology Knowledge sharing Open access / open science Open source hardware
  • 2. What the Soil Food Web does… • Organisms do the work in soil and compost - – make nutrients plant-available – build soil structure – suppress diseases and pests • Organisms are fed and supported by plants through root exudates and dead plant material • Protozoa, nematodes, earthworms and micro-arthropods eat bacteria and fungi to release nutrients in plant-available forms nothing
  • 3. MICROBES AND SOIL FORMATION  3.4.1 Living Organisms  ●Bacteria -  "A teaspoon of productive  soil generally contains  between 100 million and 1  billion bacteria. That is as  much as two cows per acre."  - Elaine R. Ingham, NRCS  1. Decomposers  2. Mutualists - nitrogen-fixers  3. Pathogens  4. Chemoautotrophs (or lithotrophs)
  • 4. SOIL FERTILITY  Bacteria can transform minerals  Bacteria can degrade organic matter  Many reactions possible  CHO  CO2 + H2O  SO4SH2S  NO3N2  NH3 N2  NH3  NO3
  • 5. SOIL BACTERIA  Different bacteria work together  eg NH3 + NO3 N2  CH3  CH4 (need other bacteria producing H)  Potential for cleaning CH3-Br
  • 6. RELEVANCE OF ASTROBIOLOGY  More bacteria remain to be discovered  Astrobiology can provide insight in processes at the interface living – non living  New leads for processes useful for agriculture  New leads for environment cleaning
  • 7. SHARING KNOWLEDGE  Agriculture networks of knowledge  CIARD, GFAR, AIMS, CGIAR
  • 9. SHARING KNOWLEDGE What we want: bottom-up networks of practitioners, farmers, students, citzen scientists Use for example iHUB (kenya) Use contacts of open science for development group Use contacts from Open Knowledge global network
  • 11. ACCESS TO THE INTERNET FAST GROWING
  • 12. DEVELOPING WORLD CATCHING UP  65% of the people who get onto the internet today come from emerging markets (mainly using wireless)  Meanwhile, only 24% of the people in emerging markets are online.  That means that whether the West realizes it or not, the internet focus has already shifted, the ripples of this are only now being felt.
  • 13. TRANSFER OF TECHNOLOGY: AGRICULTURAL METHODS  Easy to follow methods  Local production organic fertilizers  Local sources  Low-cost materials  Easy measurements  In short RockinSoils
  • 14. TRANSFER OF TECHNOLOGY: MEASURING TOOLS  Open Source Hardware OSH  Key technology 3D Printing Low cost spectrometer, microscope, pH meter, conductivity, test tubes, containers and more
  • 15.
  • 16.
  • 17. OPEN SOURCE HARDWARE ‘Just as the power of the open-source design paradigm has driven down the cost of software to the point that it is accessible to most people, the rise of open-source hardware is poised to drive down the cost of doing experimental science to expand access to everyone’  You can download designs from  Thingiverse or another repository (e.g. grabcad, github.com, 3Dhacker.com,  appropedia.org, etc.)
  • 18. OPEN-SOURCE COLORIMETER  in environmental science, colorimeters are used to monitor the levels of nitrates, phosphates,metals and other compounds present in effluent entering the natural environment
  • 19. PUBLIC LABORATORY (HTTP://PUBLICLABORATORY.ORG/HOME) The initial versions of the Public Laboratory spectrometers were made by crudely taping a small black paper case and a slice of D VD -R to the back of an Android phone Now using the powerful tool of open-source 3-D printing, you can print a superior version of the Public Laboratory spectrometer (v3.0)
  • 21. 3-D PRINTABLE OPEN-SOURCE MICROSCOPE WITH SMARTPHONE
  • 22. 3D PRINTED EQUIPMENT Laser cut test tube racks A) assembled rack B) Pattern for cutting But also possible PCR apparatus
  • 24. HACKTERIA.ORG There are a growing number of organizations devoted to producing O S H for science. One such group is Hackteria.org, which was designed as a community platform to encourage the collaboration of scientists, hackers and artists to combine their expertises