13. 1-Adamantanecarboxylic acid + toluene (8ml per 1 gram) Lauric acid or palmiticacid glass reactor with a temperature-stabilized jacket 96 ⁰C 30 minutes Glycidol acid:glycidol (1:1,8) Purification and Filtration Methods Preparation of MAG Solvent system Non-solvent system Chromium (III) acetate hydroxide0,5% w/w 96 ⁰C 30 minutes 96 ⁰C 120 minutes 14. Methods Antimicrobialassay Bacillus subtilis Staphylococcus aureus Bacillus cereus Micrococcus luteus EnterococcusFaecalis Gram + Micrococcus luteus CCM 732 Escherichia coli Citrobacterfreundii Serratiamarcescens Salmonella enterica Pseudomonas aeruginosa Gram - Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420 15. Methods Antimicrobialassay Bacillus subtilis Staphylococcus aureus Bacillus cereus Micrococcus luteus EnterococcusFaecalis Gram + Micrococcus luteus CCM 732 Escherichia coli Citrobacterfreundii Serratiamarcescens Salmonella enterica Pseudomonas aeruginosa Gram - Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420 16. Methods Antimicrobialassay Dilution of MAG in MPB (25 - 1500 µl/ml) 200µl MPB with MAG + 5 µl of bacterial suspension Incubation in plate reader Absorbance readings (600nm) Every 30 minutes24 hours Growth curves 24. Results Petridishes Used to confirmtheresults MAG A of Iva-1000µg/ml MAG A of Gabriel - 1000 µg/ml Nisin - 500µg/ml MAG A of Iva + Nisin–100MAG + 300N µg/ml MAG A of Gabriel + Nisin-100MAG + 300N µg/ml MAG Lauric - 1000µg/ml MAG A of Gabriel + Nisin - 200MAG + 200N µg/ml
