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Lysander Borrero
 Eduardo Rivera
   MBRS-RISE
  UPR at Cayey
 Dr. Javier Arce
 Background Information
 Objective
 Hypothesis
 Methodology
 Results
 Discussion
 Future Work
 References
 Nitrate:
   Composition of one nitrogen atom and three oxygen
    atoms
   Soluble in water at standard temperature and pressure




   http://www.chemistry.wustl.edu
   /~courses/genchem/Tutorials/Io   http://sci.waikato.ac.nz/farm/images/a
   ns/images/nitrate3.jpg           tomic%20structure%20of%20nitrate.p
                                    ng
 Nitrates are important for plants and algae to grow


 Fertilizers


 High levels of nitrate can cause:
   Methemoglobinemia-Blue baby (Austin, 1999)


   Cancer
 The nitrate levels will rise after a precipitation event in
  studied water bodies.
 Measure the levels of nitrates in water bodies after an
  extreme precipitation event.
Lake Sample   River Sample
 Collect samples
 TNT 835 (Dimethylphenol
 Method)
   Sample water (1 mL)
   Solution A (200 µL)


 Spectrophotometer
   Insert the vial
   Reads the barcode
   Measures nitrate levels
Results
Date            Time      Nitrate Concentration   (mg/L)


28/april/2012   9:00 am   0.079


28/april/2012   4:45 pm   0.111


29/april/2012   7:15 am   0.122


29/april/2012   4:30 pm   0.113


30/april/2012   9:30 am   0.147


30/april/2012   6:38 pm   0.137


1/may/2012      6:47 am   0.137


1/may/2012      7:03 pm   0.469


2/may/2012      9:00 am   0.061


2/may/2012      6:48 pm   0.258
Date            Time       Nitrate Concentration (mg/L)


28/april/2012   11:35 am   0.303


28/april/2012   5:32 pm    0.573


29/april/2012   11:45 am   0.211


29/april/2012   6:01 pm    0.540


30/april/2012   6:28 am    0.628


30/april/2012   6:27 pm    0.791


1/may/2012      6:37 am    0.706


1/may/2012      7:12 pm    0.470


2/may/2012      8:24 am    0.600


2/may/2012      8:21 pm    0.730
Levels of nitrate in the
   river (blue bar)
           vs.
levels of nitrate in the
    lake (pink bar)
http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=01_00065&format=img_default&site_no=
50055225&set_arithscale_y=on&begin_date=20120425&end_date=20120502
Lake Sample
         Before precipitation:
         from 4/28/2012am to
              4/30/2012 am
                    vs.
       After precipitation: from
            4/30/2012pm to
               5/2/2012pm
River Sample
          Before precipitation:
          from 4/28/2012 am to
               4/30/2012 am
                     vs.
        After precipitation: from
             4/30/2012 pm to
               5/2/2012 pm
Lake Water                     River Water
 Concentration of nitrates     Concentration of nitrates
  0.079-0.469                    0.211-0.791
 Nitrate level increased       Nitrates levels increased
  after precipitation event      after precipitation event
 Nitrate level increased in    Nitrate level increased in
  5/1/2012 pm                    4/30/2012 pm
 Precipitation can be a factor in the levels of nitrates.


 Possible factors that affected our results:
   Periods between samples
   Storage of the samples
   Agricultural and rural areas
   Artificial lake
 Compare extreme dry periods vs. extreme
 precipitation event.

 Collect more samples for a longer period of time.


 Collect from the same water body, but on different
 sites.
 Dr. Javier Arce


 Ruby Otero


 RISE Program
   Dr. EneidaDíaz
   Dr. Elena González
   Dr. Roberto Ross
 Hach Company. 2007. Nitrate. Revised 2010. Doc
  316.53.01070. (p) 1-6

 Kreitler C, Jones D. 1975. Natural Soil Nitrate: The Cause of
  Nitrate Contamination of Ground Water in Runnels
  County, Texas. 13 (1)

 Self JR, Waskom RM. July 1995. Nitrates in Drinking Water.
  Revised October 2008. Fact Sheet No. 0.517
 Austin A. 1999. Infantile Methehemoglobinemia:
 Reexamining the Role of Drinking Water Nitrates.
 Environmental Health Perspectives [Internet]; [Published
 1999 June 1]. Volume(107):[approximately 4 p.]. Available
 on
 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566680/?
 page=1
 United States Geological Survey
   http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=01_00065
    &format=img_default&site_no=50055225&set_arithscale
    _y=on&begin_date=20120425&end_date=20120502
   http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=03_0004
    5&format=img_default&site_no=50055225&set_arithscal
    e_y=on&begin_date=20120425&end_date=20120502
Course 4997 Final Presentation

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Course 4997 Final Presentation

  • 1. Lysander Borrero Eduardo Rivera MBRS-RISE UPR at Cayey Dr. Javier Arce
  • 2.  Background Information  Objective  Hypothesis  Methodology  Results  Discussion  Future Work  References
  • 3.  Nitrate:  Composition of one nitrogen atom and three oxygen atoms  Soluble in water at standard temperature and pressure http://www.chemistry.wustl.edu /~courses/genchem/Tutorials/Io http://sci.waikato.ac.nz/farm/images/a ns/images/nitrate3.jpg tomic%20structure%20of%20nitrate.p ng
  • 4.  Nitrates are important for plants and algae to grow  Fertilizers  High levels of nitrate can cause:  Methemoglobinemia-Blue baby (Austin, 1999)  Cancer
  • 5.  The nitrate levels will rise after a precipitation event in studied water bodies.
  • 6.  Measure the levels of nitrates in water bodies after an extreme precipitation event.
  • 7. Lake Sample River Sample
  • 8.  Collect samples  TNT 835 (Dimethylphenol Method)  Sample water (1 mL)  Solution A (200 µL)  Spectrophotometer  Insert the vial  Reads the barcode  Measures nitrate levels
  • 10. Date Time Nitrate Concentration (mg/L) 28/april/2012 9:00 am 0.079 28/april/2012 4:45 pm 0.111 29/april/2012 7:15 am 0.122 29/april/2012 4:30 pm 0.113 30/april/2012 9:30 am 0.147 30/april/2012 6:38 pm 0.137 1/may/2012 6:47 am 0.137 1/may/2012 7:03 pm 0.469 2/may/2012 9:00 am 0.061 2/may/2012 6:48 pm 0.258
  • 11. Date Time Nitrate Concentration (mg/L) 28/april/2012 11:35 am 0.303 28/april/2012 5:32 pm 0.573 29/april/2012 11:45 am 0.211 29/april/2012 6:01 pm 0.540 30/april/2012 6:28 am 0.628 30/april/2012 6:27 pm 0.791 1/may/2012 6:37 am 0.706 1/may/2012 7:12 pm 0.470 2/may/2012 8:24 am 0.600 2/may/2012 8:21 pm 0.730
  • 12. Levels of nitrate in the river (blue bar) vs. levels of nitrate in the lake (pink bar)
  • 14. Lake Sample  Before precipitation: from 4/28/2012am to 4/30/2012 am vs.  After precipitation: from 4/30/2012pm to 5/2/2012pm
  • 15. River Sample  Before precipitation: from 4/28/2012 am to 4/30/2012 am vs.  After precipitation: from 4/30/2012 pm to 5/2/2012 pm
  • 16. Lake Water River Water  Concentration of nitrates  Concentration of nitrates 0.079-0.469 0.211-0.791  Nitrate level increased  Nitrates levels increased after precipitation event after precipitation event  Nitrate level increased in  Nitrate level increased in 5/1/2012 pm 4/30/2012 pm
  • 17.  Precipitation can be a factor in the levels of nitrates.  Possible factors that affected our results:  Periods between samples  Storage of the samples  Agricultural and rural areas  Artificial lake
  • 18.  Compare extreme dry periods vs. extreme precipitation event.  Collect more samples for a longer period of time.  Collect from the same water body, but on different sites.
  • 19.  Dr. Javier Arce  Ruby Otero  RISE Program  Dr. EneidaDíaz  Dr. Elena González  Dr. Roberto Ross
  • 20.  Hach Company. 2007. Nitrate. Revised 2010. Doc 316.53.01070. (p) 1-6  Kreitler C, Jones D. 1975. Natural Soil Nitrate: The Cause of Nitrate Contamination of Ground Water in Runnels County, Texas. 13 (1)  Self JR, Waskom RM. July 1995. Nitrates in Drinking Water. Revised October 2008. Fact Sheet No. 0.517
  • 21.  Austin A. 1999. Infantile Methehemoglobinemia: Reexamining the Role of Drinking Water Nitrates. Environmental Health Perspectives [Internet]; [Published 1999 June 1]. Volume(107):[approximately 4 p.]. Available on http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566680/? page=1
  • 22.  United States Geological Survey  http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=01_00065 &format=img_default&site_no=50055225&set_arithscale _y=on&begin_date=20120425&end_date=20120502  http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=03_0004 5&format=img_default&site_no=50055225&set_arithscal e_y=on&begin_date=20120425&end_date=20120502

Editor's Notes

  1. Impotance: for plants as it is a source of nitrogen which forms the building blocks of molecules such as amino acids and DNA.
  2. A form of hemoglobin that doesn’t carry oxygen through out the body called methemoglobin. N-nitroso compound found in the gastric juices on the stomach
  3. Describiralrededores, como se cogieronlasmuestras…
  4. Dimethylphenol method: TNT 835 – Sulfuric and phosphoric acid Solution A – isopropanol
  5. Diferenciasignificativa p<0.001 t-test
  6. Unted States Geological Survey Rain gauge:  gather and measure the amount of liquid precipitation over a set period of time.
  7. Nitrates are more concentrated below or near the area of waste accumulation or disposal such as manure piles, feedlots, septic tank disposal fields, cesspools, privies, etc. Excess nitrates also are more apt to be found in ground water under low areas and waterways that collect or conveyFactors that maybe affected our results may have been the temperature because Nitrate levels generally are highest following wet periods and lowest, even down to zero nitrates, during dry periods which may cause a false sense of security.Nitrate levels may have decrease because If nitrate levels drop it can mean two things. One possibility is that the bacteria that break down either ammonia or nitrite have been killed off.