Ennui Garbage in, garbage out Running analysis over the wrong datasets E.g. Identifying chicken proteins in mouse cells. Configuration traditionalism Not changing the parameters of BLAST. Ever. Top list ennui If there is a list only looking at the first one. Look no further than the first Blast hit / first Google hit. Arbitrary cut-offs on rank-ordered result list Absolute truth above, absolute falsehood below E.g. differentially expressed genes in microarray analyses.