Platelets are living but terminal cytoplasmic portions of marrow megakaryocytes.They have no nucleus but have alpha granules which release growth factors.

1. USE OF PLATELET RICH PLASMA
IN AESTHETIC SURGERY
DR.R.KARTHIK,
Cosmet ic Surgeon,
Chennai Plas t ic Surgery

2. INTRODUCTION
• Platelets are living but terminal cytoplasmic portions of
marrow megakaryocytes
• They have no nucleus but have alpha granules which
release growth factors
Coagulation
Tissue
Healing

3. PLATELET RICH PLASMA BIOLOGY
PLATELET DERIVED GROWTH
FACTORS
Initiates connective tissue healing.
Increases mitogenesis, angiogenesis
and macrophage activation
 Differentiation of fibroblasts
VASCULAR ENDOTHELIAL
GROWTH FACTOR
Possess potent angiogenic properties
Enhances vascular permeability
EPIDERMAL GROWTH FACTOR
Induces epithelial development
Promotes angiogenesis
TRANSFORMING GROWTH
FACTOR BETA
Increases chemotaxis and mitogenesis.
Stimulates deposition of collagen
INSULIN LIKE GROWTH FACTOR
EPIDERMAL GROWTH FACTORS
Induces epithelial development
Promotes angiogenesis

4. PLATELET RICH PLASMA
 Normal count : 2 X 105 / μL PRP : 2 million / μL
 To be considered PRP, must be 4X or greater above
baseline levels
 Increasing the concentration of bioactive proteins acts as
a catalyst for accelerating the wound healing process

9. ISOLATION OF PRP
• Early technique of isolation was
difficult because of equipment size,
cost, need for perfusionist & large
volume of blood
• The new technique is easy with the
help of AUTOMATED DUAL SPIN
PROCESS MACHINE
HARVEST SmartPREP®2

10. ISOLATION OF PRP

11. HARVEST SmartPReP2 SYSTEM
20 – 60 cc of blood withdrawn & placed in a
tube containing citrate phosphate dextrose as
an anticoagulant
Automated Multibiologic
Platform System
(15 mins. processing)
Unsurpassed Autologous
Biologic Concentrate

12. APPLICATIONS OF PRP
 SKIN REJUVENATION
Fine wrinkles around the eyes
Nasolabial grooves
Wrinkles on the lips
Acne marks
Wrinkles on the forehead & neck
Bags and dark circles under the eyes
Loose, saggy skin
 HAIR RESTORATION
 FAT FILLS

23. PRP IN FAT FILLS

24. ACTION OF PRP
• Stimulates mesenchymal stem cells found in fat deposits
(PDGF)
• Increases the adhesion of cells (PDGF)
• Increases Revascularization (PDAF)
• Stimulates Active fibroblast & collagen matrix formation
(PFGF)
• Act as stable scaffolding

25. ADVANTAGES
• Increases the graft uptake
• Increases survival rate & longevity of graft
• Decreases the liponecrosis
• Decreases Lipid cyst formation
• Decreases micro calcification especially in large volume
augmentation (breast & buttocks)

26. Pre - op 1 week post - op