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Location of BCL:-
Name of department :-Agril. Entomology
Name of college :- K. K. Wagh college of agril.
Name of farm area:- Puriya park, Saraswati nagar
Name of university :- M.P.K.V ,Rahuri
Experiential learning Module Activity
Course No. AEL-ENT-486
Title : Mass production of bioagent and biopesticides
Major Department: Agril. Entomology
Associated subjects: Economics, Extension Education
No. of students Registered: 14
Activities carried out by students:
Rearing of host insect like Corcyra cephalonica, Mealy bug
Rearing of Bioagents like Trichogramma, Cryptolaemus,
Mass production of HaNPV and SlNPV
Mass production of Metarhzium, Nomuraea, Verticelium,
Beavaria,Trichoderma & Psudomonus etc.
STERILIZATION ROOM :
1) use of this room for
sterilization through the
2) This room should be
well ventilated by fitting
3) In this room should be
2 autoclave, a
horizontal & a vertical
autoclave & Hot air
each floor, or at a
minimum in a
in each building,
Media preparation room :
This room should get a sufficient & continuous
supply of single & double-distillation unit ,pressure
cooker , Exhust fan.
Inoculation room :
1) In this room the inoculation cabinet laminar air flow can be
2) The floor of this room should be covered with linoleum or
tiles to facilitate proper cleaning.
3) It is essential that the atmosphere in this room should be
free from contaminants.
4) This room should preferably be air-conditioned because it
ensures good air-circulation & helps in maintaining
GROWTH ROOM :
1)In this room all inoculated bottles with spores
2)This room should be in sterile condition
3)Air condition should be there to control
4)The following fungi are grow in this room :
STORE ROOM :
1) In this room store in Chemicals
2) Then Chemicals are Agar agar,
ethyl alcohol, peptone,
streptomycine, talc powder
3) The chemical rack should be
arrange with A to Z
alphabetical latters so its easy
to find chemicals.
1) Grinding of different
2) Mixing the mycelium
culture with talcum
3) Drying of blended
4) Equipment: laboratory
two types of microscope
I. Steriozoom microscope
II. binocular microscope
INSECT REARING ROOM :
1) In this room rearing of
helicoverpa larvae &
2) Rearing of moth of
helicoverpa & spodoptera
moth in glass chamber.
3) Rearing of mealy bug on
pumpkin as a food for
cryptolamous its use in off
season when the mealy
bug can not be available.
MICROVAVE OVEN :
Microwave oven use to heat the content of
NPV ROOM :
1) This room is also called as virus
2) All viral processed taken in this
3) NPV made in this room.
4) The Infected food i.e.
.gram, A.diet given to the
helicoverpa larvae given in this
5) Equipment : centrifugal,
laboratory blender, rack,
exhaust, fan & furniture.
6) ventilation system provide in
this room for exhaust of dirty
WASHING ROOM :
1) The room should be
provided with necessary
service lines as water
,cleaning materials i.e.
soap, sodium chloride .
2) In two side of the room a
washing sink can be
3) The drainage & drying
racks could be
accommodated in this