Coronal and radicular pulp
Apical foramen
Accessory canal
Functions of dental pulp
Components of dental pulp
Functions of pulpal extracellular matrix
Organization of cells in the pulp
The principle cells of the pulp
The pathways of collagen synthesis
Matrix and ground substances
Vasculature and lymphatic supply
Innervation of Dentin- pulp complex
Disorders of the dental pulp
Advances in pulp vitality testing
High Profile Call Girls Jaipur Vani 8445551418 Independent Escort Service Jaipur
Dental pulp
1. Dr. Ashok Ayer
Assistant Professor
Department of Conservative Dentistry & Endodontics
B.P.Koirala Institute of Health Sciences, Dharan, Nepal
2. Contents:
Introduction
Coronal and radicular pulp
Apical foramen
Accessory canal
Functions of dental pulp
Components of dental pulp
Functions of pulpal extracellular matrix
Organization of cells in the pulp
The principle cells of the pulp
The pathways of collagen synthesis
Matrix and ground substances
Vasculature and lymphatic supply
Innervation of Dentin- pulp complex
Disorders of the dental pulp
Advances in pulp vitality testing
Conclusion
3. Dental Pulp
Occupies the center of each tooth.
Soft connective tissue that supports the dentin.
Total 52 pulp organs; 32: Permanent, 20: Primary
Total Volume of all permanent teeth pulp organs is
0.38 cc.
Mean volume of a single adult human pulp is 0.02 cc.
4. Maxillary
(Cubic Centimeter)
Mandibular
(Cubic Centimeter)
Central Incisor 0.012 0.006
Lateral Incisor 0.011 0.007
Canine 0.015 0.014
First Premolar 0.018 0.015
Second Premolar 0.017 0.015
First Molar 0.068 0.053
Second Molar 0.044 0.032
Third Molar 0.023 0.031
Orban’s Oral histology & embryology: Pulp; Department of Oral Surgery,
Newcastle - Tyne, England
6. Radicular Pulp:
The radicular portion of the pulp organs are
continuous with the periapical connective tissue
through the apical foramen or foramina.
As growth proceeds, more dentin is formed, so that
when the root of teeth are matured the radicular pulp
is narrower.
The apical pulp canal becomes smaller also because of
apical cementum deposition.
7. Apical foramen:
Average size of apical foramen of the maxillary teeth
in the adult is 0.4 mm
Mandibular teeth 0.3 mm
Sometimes it is found on the lateral side of the apex
although the root itself is not curved.
Frequently there are two or more foramina separated
by a portion of dentin and cementum or by cementum
only.
8. Accessory canal:
Leading from the radicular pulp laterally through the
root dentin to the periodontal tissue.
May be seen anywhere along the root but are most
numerous in the apical third of the root.
Clinically significant in spread of infection, either
from the pulp to the periodontal ligament or vice
versa.
9. Occur in areas where there is premature loss of root
sheath cells; these cells induce the formation of
odontoblasts which form dentin.
May also occur where the developing root encounters
a blood vessel.
11. Inductive:
Interact with the oral epithelial cells
Differentiation of the dental lamina and enamel
organ formation.
Cells of pulp + blood vessels & nerves
provides the tooth vitality
12. Formative:
Produces dentin that surrounds and protects the pulp.
Pulpal odontoblasts develop the organic matrix and
function in its calcification.
13. Nutritive:
Blood vascular system of the pulp; nourishes dentin
through the odontoblasts and their processes.
Protective:
Sensory nerve respond to pain
Nerves initiate reflexes that control circulation in the
pulp.
14. Defensive or reparative:
First line of defense to injuries and infection of
dentine
Tertiary dentine
Immuno-competent
Clearance of toxic substances
16. Components of dental pulp
CELLS (odontoblast, fibroblast,
undifferentiated cell, macrophage,
dendritic cell)
FIBERS AND GLYCOPROTEIN (collagen
type I, III, no elastic fiber, fibronectin)
GROUND SUBSTANCES
(glycosaminoglycans, chondroitin sulfate
proteoglycan)
BLOOD VESSELS, NERVES, LYMPH
VESSELS
17. Maintain tissue’s physical properties and integrity
Control of growth and development and repairs
Control of cell migration
Control of diffusion of macromolecules
Functions of pulpal extracellular matrix
18. Collagen in dental pulp
Concentration varies from species to species, 32% in human
pulp.
Higher content in the middle and apical pulp.
Total collagen decreases with age.
Interestingly high level of collagen type III. (43%) : vascular
content, tissue extensibility (cf. Elastin)
Absence of elastin (except in b.v.).
19. Adhesive glycoproteins in dental pulp
Fibronectin found in
predentine NOT mature
dentine.
Fibronectin present in
pulp and dental papilla.
Fibroblasts synthesize
pulpal fibronectin.
Fibronectin is expressed
during reparative
dentinogenesis.
Immunoreactive fibronectin molecules detected
along the border of predentine and between
odontoblast (Yoshiba et al., 1994)
20. Glycosaminoglycans in dental pulp
Chondroitin sulfate, dermatan sulfate, hyaluronic
acid present
Amount of uronic acid decreases with age
Total GAG decreases with reduced dentinogenic
activity
Decorin may involve in mineral nucleation at the
mineralization front
22. Four distinct zones:
1. The odontoblastic zone at the pulp periphery
2. A cell free zone of Weil beneath the odontoblast;
prominent in the coronal pulp
3. A cell rich zone; high cell density
4. The pulp core; major vessels and nerves
23.
24. The principle cells of the pulp:
Odontoblasts
Fibroblast
Undifferentiated mesenchymal cells
Macrophages
Immunocompetent cells
25. Odontoblasts:
The most distinctive cells of the dental pulp
Form a layer lining the periphery of the pulp and have
a process extending into the dentin
Arranged in palisade pattern of three to five cells deep
59,000 to 76,000 per square milimeter in coronal
dentin, with a lesser number in root dentin.
26. Active cells:
Elongated, basal nucleus, much basophilic cytoplasm,
promonent golgi zone.
Resting cell:
Stubby, little cytoplasm, more hematoxophilic nucleus.
27. Odontoblast process begins at the neck of the cells
just above the apical junctional complex where the cell
gradually begins to narrow as it enters predentin.
The process is devoid of major organelles but does
display an abundance of microtubules and filaments
arranged in a linear pattern along its length.
28. The pathways of collagen synthesis:
The spherical distensions contain free polypeptides
that assemble as a triple helix in the cylindrical
distensions to form the procollagen molecule.
The cylindrical distension bud off as secretory
granules.
Secretory granules that are transported toward the
odontoblast process, where their content is released.
30. Some types (of 15) of known collagen
Type Molecular Tissue distribution
Fibril-forming I [a1(I)]2 a2(I) bone, skin, tendon, ligaments
(90%) of body collagen
II [a1(II)]3
cartilage, intervertebral disc,
notochord, vitreous humor of eye
III [a1(III)]3 skin, blood vessels, internal organs
V [a1(V]2 a2(V) as type I
XI [a1(XI] a2(XI) a3(XI) as type II
Fibril-associated IX [a1(IX] a2(IX) a3(IX) cartilage (with type II)
XII [a1(XII)]3 tendon, ligaments (with some type I)
Network-forming IV [a1(IV)]2 a2(IV) basal laminae
VII [a1(VII)]3 anchoring fibrils beneath stratified
squmous epithelia
33. Aggrecan mechanical support
(cartilage)
Betaglycan binds TGF-beta
(cell surface*, matrix)
Decorin binds type I and (CNT)
TGF-beta
Perlecan basal laminae
(basal laminae)
Syndecan-1 binds FGF
(cell surface*)
* = Integral membrane proteoglycan
Some known proteoglycans:
34. Junctions occur between adjacent odontoblasts
involving
Gap junctions
Occluding zones (Tight junctions)
Desmosomes
The actin filaments inserting into the adherent
junction are prominent and form a terminal cell web.
35. This junctional complex does not form a zonula,
completely encircling the cell, as occurs in epithelia;
(it is focal, and there is some debate whether it can
restrict the passage of molecules and ions from the
pulp into the dentin layer)
Serum proteins seem to pass freely between
odontoblasts and are found in dentin
36. Fibroblasts:
Greatest number in the pulp
Numerous in coronal pulp where they form the cell-
rich zone.
The function is to form and maintain pulp matrix.
37. Undifferentiated Ectomesenchymal Cells:
Represents the pool from which the connective tissues
of the pulp are derived.
Depending upon the stimulus these cells may give rise
to odontoblasts and fibroblasts.
In older pulp they diminish, thereby reducing the
regenerative potential of the pulp.
38. Macrophages
Located throughout the pulp center.
Involved in the elimination of dead cells, the presence
of which indicates that turnover of dental pulp
fibroblast occurs.
40. Dendritic Cells
Bone marrow derived, antigen presenting dendritic
cells.
Beneath the odontoblast layer.
They capture and present foreign antigen to the T
cells.
41. Cells participate in immunosurvillance and increase
in number in carious teeth.
Infiltrate odontoblast and project their processes into
the tubules.
8% of total cell population.
42. Matrix and Ground Substance
Principally Type I and Type III collagen.
Composed of glycosaminoglycans, glycoproteins, and
water.
Overall collagen content increases with age.
43. The greatest concentration of collagen generally
occurs in the most apical portion of the pulp.
Significance:
During pulpectomy; Engaging the pulp with a barbed
broach in the region of apex affords a better
opportunity to remove the tissue intact.
44. Vasculature and Lymphatic Supply
Circulation establishes the tissue fluid pressure.
One or sometimes two vessels of arteriolar size
(about 150µm) enter the apical foramen with the
sensory and sympathetic nerve bundles.
Smaller vessels, without any accompanying nerve
bundle, enter the pulp through the minor foramina.
46. The arterioles occupy a central position within the
pulp and, as they pass through the radicular portion of
pulp, give off smaller lateral branches.
Occasionally U- looping of pulpal arterioles is seen,
and this anatomic configuration is thought to be
related to the regulation of blood flow.
47. Pulp tissue is highlyvascularized.
40-50 ml/min/100g
(Kim, 1985)
48. Some terminal capillary loops extend upward between
the odontoblasts to abut the predentin if
dentinogenesis is occurring.
Located on the periphery of the capillaries at random
intervals are pericytes.
Pericytes are contractile cells capable of reducing
the size of the vessel lumen.
49. Anastomosis are point of direct communication
between the arterial and venous sides of the
circulation.
Lymphatic vessels also occur in the pulp tissue, they
exit via one or two large vessels through the apical
foramen.
50. Sympathetic adrenergic nerves terminate in relation
to the smooth muscle cells of the arteriolar walls.
Afferent free nerve endings terminate in relation to
arterioles, capillaries and veins and serve as effectors
by releasing various neuropeptides that exert an
effect on the vascular system.
51. Dental pulp interstitial fluid (ISF) and exchange of substances between plasma
and ISF. (* values from Tonder and Kvinnsland, 1983; Ciucchi et al., 1995)
(5.5-10.3 mm Hg*)
(43 mm Hg)
(20 mm Hg)
(35 mm Hg)
Hydrostatic pressure
in dental pulp
52. Innervation of Dentin- Pulp Complex
Nerve enter the pulp through
apical foramen, along the
afferent blood veessels, and
together from the
neurovascular bundle.
Each nerve fiber has been
estimated to provide at least
eight terminal branches.
53. These branches ultimately contribute to an
extensive plexus of nerves in the cell free
zone just below the cell bodies of the
odontoblasts in the crown portion of the
tooth.
55. This plexus of nerves, which is called the
subodontoblastic plexus of Raschkow, occupies the
cell- free zone of Weil and can be demonstrated in
silver nitrate stained sections under the light
microscope or by immunocytochemical techniques.
56. The nerve bundles that enter the tooth pulp consist
principally of :
Sensory afferent nerves of the trigeminal
nerve
and
Sympathetic branches from the superior
cervical ganglion.
57. As the nerve bundle ascends coronally;
The myelinated axons gradually loose their
mylein coating,
So that a proportional increase in the number
of unmyelinated axons occurs in the more
coronal aspect of the tooth.
58. A-delta fibers
Conduction velocity 2-30 m/s
Lower threshold
Involved in fast, sharp pain
Stimulated by hydrodynamic
stimuli
Sensitive to ischemia
Sharp pain
C fibers
Conduction velocity 0-2 m/s
Higher threshold
Involved in slow, dull pain
Stimulated by direct pulp damage
Sensitive to anesthetics
Dull pain
Types and properties of pulpal sensory nerve fibers
A-beta fibers
Conduction velocity 30-70 m/s
Very low threshold, non-noxious
sensation
50% of myelinated fibers in pulp
Functions not fully known
Non-myelinated sympathetic
fibers
Conduction velocity 0-2 m/s
Post-ganglionic fibers of superior
cervical ganglion
Vasoconstriction
59. A small number of axons pass between the
odontoblast cell bodies to enter the dentinal tubules
in proximity to the odontoblast process.
60.
61. Possible mechanisms of dentine sensitivity
Hydrodynamic mechanism
(Gysi, 1900; Brannstrom, 1963)
62. Pulp venules
STIMULATION
Increased pulp
interstitial fluid
Increased pulp
pressure
Increased tubular
fluid flow
Release of
inflammatory
agents?
Increased blood
viscosity and rbc
congestion in capillary
bed
Increased A-V shunt
blood flow
Outward dentinal
fluid flow and
aspiration of
odontoblasts
CNS, Pain, Reflexes
Vasodilation, Increased permeability
Pulpal axonal reflex due to dentine stimulation
Without infection,
Vascular changes could
be resolved.
Axon
reflex
SP, CGRP
Dentine
64. Pulp Stones
Pulp stones, or denticles, frequently are found in pulp
tissue.
Discrete calcified masses that have calcium phosphorus
ratios comparable to that of dentin.
More frequently at the orifice of the pulp chamber or
within the root canal.
65. Concentric layers of mineralized tissue formed by
surface accretion around blood thrombi, dying or
dead cells, or collagen fibers.
Occasionally a pulp stone may contain tubules and
be surrounded by cells resembling odontoblasts.
66. Such stones are rare and, if seen, occur close to the
apex of the tooth. Such stones are referred to as ‘true’
pulp stones as opposed to ‘false’ stones having no cells
associated with them.
67. If during the formation of a pulp stone, union occurs
between it and the dentin wall, or if secondary dentin
deposition surrounds the stone, the pulp stone is
called an attached stone.
68. The presence of pulp stones is significant in that
They reduce the overall number of cells within
the pulp
and
Act as an impediment to debridement and
enlargement of the root canal system during
endodontic treatment.
69.
70. Age Changes
Decrease in the volume of pulp chamber and root
canal brought about by continued dentin deposition.
On occasion can appear to be obliterated almost
completely.
From about the age of 20 years, cells gradually
decrease in number until age 70, when the cell density
has decreased by about half.
71. Fibrosis is due to aging & Injury.
Increase in collagen fibers’
bundles which becomes more
evident with the decrease in pulp
size
72. Lose and a degeneration of myelinated and
unmyelinated axons that correlate with an age-
related reduction in sensitivity.
Irregular areas of dystrophic calcification,
especially in central pulp.
Gradual reduction of tubule diameter.
73. The continued deposition often leads to complete
closure of the tubule;
as can be seen readily in a ground section of
dentin, because the dentin becomes translucent
(or sclerotic).
Sclerotic dentin is found frequently near the root
apex in teeth from middle aged individuals.
74. Pulpitis
Acute or chronic.
Partial or total.
Open or closed.
Exudative or suppurative.
Reversible or irreversible.
75. Pulpitis is a dynamic process and presents a
continuous spectrum of changes reflecting
interplay between cause and host
defenses.
Poor correlation between microscopic
changes & clinical symptoms.
76. Pulpitis: Clinical Features
Presents as pain which patient may have difficulty
in localizing to a particular tooth.
Pain may radiate to adjacent jaw, face, ear, or neck.
May be continuous for several days or may occur
intermittently over a longer period.
Pulpitis is often described as acute or chronic
based on duration and severity of symptoms.
77. Acute pulpitis
Severe throbbing, lancinating pain on thermal
stimulation or lying down, keeps patient awake.
Generally lasts 10-15 minutes but may be more or
less continuous (reversible pulpitis).
With progression, may become spontaneous &
continuous (irreversible pulpitis).
78. Chronic pulpitis
Bouts of dull aching which can last for an hour or
more.
Pain on thermal stimulation or spontaneously.
79. Pulpitis may be asymptomatic.
Most important decision clinically is whether
pulpitis is reversible or irreversible.
Decision is made based on many factors
including:
1. Severity of symptoms.
2. Duration of symptoms.
3. Size of carious lesion.
4. Pulp tests.
5. Direct observation during operative procedure.
6. Age of patient.
82. Pulpitis starts before leading organisms in
carious dentin reach pulp.
Pulpitis is not usually seen histologically
until organisms are within 1 mm of the
pulp in permanent teeth, or 2 mm in
deciduous teeth.
83. Chemical and thermal injury
During restorative procedures: frictional heat,
irritant substances.
May respond by reactionary dentin formation.
84. Barotrauma (aerodontalgia)
Flying at high altitude in unpressurized aircraft,
or rapid decompression in divers.
Attributed to formation of nitrogen bubbles in
pulp tissue or vessels.
Thought not to be a direct cause, but rather an
exacerbating cause in presence of caries.
85. Pulpitis: Histopathology
Poor correlation between microscopic changes &
clinical symptoms.
Inflammatory process may be modified by
several factors:
Nature, severity and duration of insult.
Efficiency of host defenses.
Efficiency of pulpo-dentinal complex defenses.
Special anatomy of pulp: surrounded by hard
tissue and cannot tolerate edema.
85
86. Reactionary dentin may continue to form after
onset of pulpitis if odontoblasts and pulp have not
been irreversibly damaged, and may protect pulp.
Pulpitis caused by caries starts as a localized area,
but extends throughout pulp if caries is not
treated.
86
87. If inflammation is severe, local
microcirculation may be compromised,
leading to local necrosis and suppuration
of pulp (pulp abscess), or diffuse
suppuration and necrosis.
88. Pulpitis: Chronic Hyperplastic Pulpitis
(Pulp Polyp)
Open pulpitis or chronic hyperplastic pulpitis (pulp
polyp):
Large carious cavities.
Young molar teeth with wide apices and good
blood supply.
88
89. Usually devoid of sensation on gentle probing.
Polyp consists of chronically inflamed
hyperplastic granulation tissue protruding
from pulp cavity.
May become epithelialized by spontaneous
grafting of desquamated oral epithelial cells from
saliva.
90. Pulp Necrosis
May follow pulpitis or trauma to apical blood
vessels.
Coagulative necrosis after ischemia.
90
91. Liquefactive necrosis after pulpitis;
may become gangrenous with foul odor upon
infection by putrefactive bacteria from caries.
Pulp necrosis in sickling crisis of sickle cell
anemia.
93. Effects of cavity Preparation:
Frictional heat
Desiccation
Exposure of dentinal tubules
Direct damage to odontoblast processes
Chemical treatment to exposed dentinal surface
94. Cavity preparation: speed, heat, pressure &
coolant may all cause pulp irritation.
Aspiration or displacement of odontoblasts into
dentinal tubules, with reduction of numbers.
94
95. Factors associated with the restorative material & its placement
Material toxicity
Insertion pressure
Thermal effects
Induced stresses
96. Effects subsequent to restoration
Marginal leakage
Cuspal fracture
Effects of cavity preparation & restorative materials may
further complicate pulpitis caused by caries or other
causes.
Thickness & nature of remaining dentine may affect pulp
response to dental material.
98. Pulse Oximetry
Dental sensor (a modified finger probe) that can be
successfully applied and adapted to the tooth and well
suited to detect pulsatile absorbance.
The principle: relates the absorption of light, by a
solute to its concentration and optical properties at a
given light wavelength.
99. It also depends on the absorbance
characteristics of haemoglobin in the red
and infra-red range
In the red region, oxyhaemoglobin
absorbs less light than deoxyhaemoglobin
and vice versa in the infrared region.
100. Hence one wavelength was sensitive to
changes in oxygenation and the second was
insensitive to compensate for changes in
tissue thickness, haemoglobin content and
light intensity.
101. The system consists of a probe containing a diode that
emits light in two wavelengths:
I. Red light of approximately 660 nm
II. Infra-red light of approximately 850 nm
It is also useful in cases of impact injury where
the blood supply remains intact but the nerve
supply is damaged
102. Dual Wavelength Spectrophotometry
Dual wavelength spectrophotometry (DWLS) is a
method independent of a pulsatile circulation.
The presence of arterioles rather than arteries in the
pulp and its rigid encapsulation by surrouding dentine
and enamel make it difficult to detect a pulse in
the pulp space.
103. This method measures oxygenation changes in
the capillary bed rather than in the supply vessels
and hence does not depend on a pulsatile
blood flow.
A major advantage is that it uses visible light
that is filtered and guided to the tooth
by fibreoptics
The test is noninvasive and yields objective results.
104. Laser doppler flowmetry
Laser Doppler Flowmetry (LDF) is a noninvasive,
electro optical technique,
Which allows the semi-quantitative recording
of pulpal blood flow.
The Laser Doppler technique measures blood
flow in the very small blood vessels of the
microvasculature.
105. The technique depends on the Doppler principle;
whereby light from a laser diode incident on the
tissue is scattered by moving RBC's
and
As a consequence, the frequency broadened.
106. The primary issues in pulp-vitality testing as
follows:
A non-vital post-traumatized incisor has a better
long-term prognosis;
If root canal therapy is completed before the
necrotic pulp gets infected.
107. The best outcome for the post
traumatized immature incisor is for it;
To revascularize and,
Continue normal root development, including
increased root wall thickness.
Which is not possible to assess with conventional
electrical and thermal testing
108. Conclusion
Thus the Preservation of Healthy Pulp during
operative procedures and successful management
in cases of disease are two of the most important
challenges.
109. References:
1. Seltzer and Bender's Dental Pulp; 2002 by Quintessence Publishing Co, Inc; Rev.
ed. of: The dental pulp / Samuel Seltzer, I.B. Bender. 3rd ed. c1984.
2. Oral histology; Development, Structure and Function: A.R. Ten Cate: 7th Edition
3. Orban’s Oral Histology and Embryology
4. Shafer’s Textbook of Oral Pathology; 5th Edition
5. Yamada, Y., Ito, K., Nakamura, S., Ueda, M. & Nagasaka, T. (2010). Promising cell-
based therapy for bone regeneration using stem cells from deciduous teeth, dental
pulp, and bone marrow. Cell Transplantation. [Epub ahead of print], (October
2010)
6. Gronthos, S., Mangani, M., Brahim, J., Robey, PG. & Shi, S. (2000). Postnatal
human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the
National Academy of Sciences of the United States of America, Vol.97, No.25,
(December 2000), pp. 13625- 13630, ISSN 0027-8424
7. Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, LW., Robey, PG. & Shi, S. (2003).
SHED: stem cells from human exfoliated deciduous teeth. Proceedings of the
National Academy of Sciences of the United Stases of America, Vol.100, No.10,
(May 2003), pp.5807-5812, ISSN 0027-8424
110. 8. Seo, BM., Miura, M., Gronthos, S., Bartold, PM., Batouli, S., Brahim, J.,
Young, M., Robey,PG., Wang, CY. & Shi, S. (2004). Investigation of
multipotent postnatal stem cells from human periodontal ligament.
Lancet, Vol.364, No.9429, (July 2004), pp.149-155, ISSN 0140-6736
9. Sonoyama, W., Liu, Y., Fang, D., Yamaza, T., Seo, BM., Zhang, C., Liu,
H., Gronthos, S.,Wang, CY., Shi, S. & Wang, S. (2006). Mesenchymal
stem cell-mediated functional tooth regeneration in swine. PLoS
One.Vol.1, (December 2006), pp.e79
10. Morsczeck, C., Gotz, W., Schierholz, J., Zeilhofer, F., Kuhn, U., Mohl,
C., Sippel, C. & Hoffmann, KH. (2005). Isolation of precursor cells
(PCs) from human dental follicle of wisdom teeth. Matrix Biology,
Vol.24, No.2, (April 2005), pp.155-165, ISSN 0945- 053X
11. Huang, GT., (2009). Pulp and dentin tissue engineering and
regeneration: current progress. Regenerative Medicine, Vol.4, No.5,
(September 2009), pp.697-707 ISSN 1746-076X
111. 12. D'Aquino, R., De Rosa, A., Laino, G., Caruso, F., Guida, L., Rullo, R.,
Checchi, V., Laino, L., Tirino, V. & Papaccio, G. (2009). Human dental
pulp stem cells: from biology to clinical applications. Journal of
Experimental Zoology Part B: Molecular and Developmental Evolution,
Vol. 312, No.5, (July 2009), pp. 408-15, ISSN 1552-5007
13. Batouli, S., Miura, M., Brahim, J., Tsutsui, TW., Fisher, LW., Gronthos,
S., Robey, PG. & Shi, S. (2003). Comparison of stem cell- mediated
osteogenesis and dentinogenesis. Journal of Dental Research, Vol.82,
No.12, (December 2003), pp. 976–981, ISSN 0022- 0345
14. Laino, G., D'Aquino, R., Graziano, A., Lanza, V., Carinci, F., Naro, F.,
Pirozzi, G., & Papaccio, G. (2005). A new population of human adult
dental pulp stem cells: a useful source of living autologous fibrous bone
tissue (LAB). Journal of Bone and Mineral Research, Vol.20, No.8,
(August 2005), pp.1394-1402, ISSN 0884-0431
15. Nakashima, M. (2005). Bone morphogenetic proteins in dentin
regeneration for potential use in endodontic therapy. Cytokine &
Growth Factor Reviews, Vol.16, No.3, (June 2005), pp.369-376 ISSN 1359-
6101
112. 16. Sun, HH., Jin, T., Yu, Q. & Chen, FM. (2011). Biological approaches
toward dental pulp regeneration by tissue engineering. Journal of
Tissue Engineering and Regenerative Medicine. Vol.5, No.4, (April 2011),
pp. e1-e16
17. Zavan Barbara et al. Dental pulp stem cells and tissue engineering
strategies for clinical application of odontoiatric field. Journal of
Biomaterial science and Engineering.