3. WHAT IS THE MEANING OF NANOPARTICLES?
Factor Prefix Symbol
o 10-1 deci d
o 10-2 centi c
o 10-3 milli m
o 10-6 micro μ
o 10-9 nano n
o 10-12 pico p
o 10-15 femto f
GOLD NANOPARTICLES
Actually the nanoparticles are
particles with sized between 100
and 1 nanometers
4. ZINC OXIDE (ZNO)
Molecular formula ZnO
Molar mass 81.408 g/mol
Appearance White solid
Odor odorless
Density 5.606 g/cm3
Melting point
1975 °C
(decomposes)
Boiling point 2360 °C
Solubility in water
0.16 mg/100 mL
(30 °C)
Band gap 3.3 eV
Refractive index(nD) 2.0041
Two types of crystal of zno also exist’s
due to diff. Extent of oxygen in it.
INTRODUCTION
o Zinc oxide is an inorganic compound with the formula ZnO. ZnO is
a white powder that is insoluble in water,
o In materials science, ZnO is a wide-bandgap semiconductor.
5. WHY WE CHOOSE ZNO NANOPARTICLES FOR
ECOTOXICOLOGICAL STUDIES NOT ITS LARGE PARTICLE
FORM?
o Nanomaterials have different toxicity profiles
compared with larger particles because of their
small size and also their high reactivity.
o When the particle size decreases, there is a
tendency to increase the toxicity, even if the
same material is relatively inert in bulk form
(e.g., SiO2, carbon black, TiO2, ZnO).
o The nanoparticles, due to their nanoscale,
shape, and consequently huge surface area,
may interact more efficiently with biological
systems, producing important toxicity.
o The toxicological impact of nanoparticles also
depends also on the biological target used.
NANO PARTICLES SOLUTION
POWDER FORM ZnO
6. THE PRESENT WORK IS FOCUSED ON:
• The study of the physicochemical properties of ZnO
nanoparticles prepared without and with protective
agents such as tri n-octylphosphine oxide(TOPO) and
brij-76 before and after contact with the culture media
• The study of the Eco toxic impact of these
nanoparticles on anabaena flos-aquae and euglena
gracilis photosynthetic microorganisms.
7. EXPERIMENTAL SECTION:
SYNTHESIS OF ZnO NANOPARTICLE
o ZnO nanoparticle synthesized in the diethylene glycol(DEG) medium
by forced hydrolysis of zinc acetete. The general procedure involves
addition of zinc acetate to 80ml of polyol and water to reach a final
conc between 0.06 and 0.6 mol/lit.
o To control particle size and shape ,protective agents such as topo(tri-n-octylphosphine
oxide) and brij 76 (polyoxyethylene stearyl ether) are
used.
o Hydrolysis ratio was varied from 10 to 80
8. ECOTOXICITY OF ZNO NANOPARTICLES IS INVESTIGATE
BY USING TWO BIOLOGICAL TARGET ‘S
o All photosynthetic microorganism were selected should be able to
fulfill two conditions:
1. They are able to synthesis some metallic nanoparticles by
synthetic route.
2. They may alive for 3 months in the presence of these
nanoparticles.
EUGLENA GRACILLICS ANABENA FLOS AQUAE
9. RESULTS AND DISCUSSION:
TEM Micrographs shows that the size of ZnO
nanoparticle is the variable function of:
The nature of the protective agent added during ZnO
formation.
The hydrolysis ratio.
10. TEM MICROGRAPH FOR ZNO PREAPARED
WITHOUT ADDITION OF PROTECTIVE AGENT :
SPHERICAL SUBMICROMETER-SIZED
NANOPARTICLES
(D=0.2 ( 0.05 ΜM),
NANORODS OF ZnO
CROWNMORPHOLOGY WERE
OBSERVED AT H=300
11. TEM MICROGRAPH FOR ZNO PREAPARED WITH
ADDITION OF PROTECTIVE AGENT
IN Presence of TOPO:
SPHERICAL NANOPARTICLES
WERE OBTAINED D=2.0 ( 0.4 NM) NANORODS OF ZnO
30<LENGTH<200
SPHERICAL PARTICLES WITH
NARROW SIZE
12. TEM MICROGRAPH FOR ZNO PREAPARED WITH
ADDITION OF PROTECTIVE AGENT
IN presence of Brij-76:
• Agglomerates were obtained by coalescence of
spherical nanoparticles with size vary with H value.
13. STRUCTURE AND SURFACE CHARGE OF ZNO
NANOPARTICLES
• Before contact with
microorganisms ,zeta
potenial of ZnO NP’s with
varying PH of medium was
measured after 30 min of
contact betweennps and PH
solution. The zero point
charge for ZnO nanoparti-cle
was at PH 9. ZnO
nanoparticle was suspended
in buffer solution at PH
2,4,7,9 and 12 by addition of
HCL and NaOH soln.
There is –ve Zeta Potential b/w PH 5 TO 9
14. STUDY OF POLYSACCHARIDE INTERACTIONS WITH THE ZNO
NANOPARTICLES
o ZnO Nanopaticles
adsorption on the calcium
alginate microcapsules
was microcapsules was
estimated using EDS
analysis same type of
NP’s show different extent
of adsorption with time
function in different
medium.
o More is the
lyophilization,More is %wt
adsorption
15. PHOTOSYNTHETIC MICROORGANISM
DESCRIPTION AND CHARACTERIZATION
ANABAENA FLOS AQUAE EUGLENA GRACILLUS
It is kind of algae.it can eat
food like animal and
photosynthesize like plants
do.
It is also called blue green
bacteria that obtain their
energy through
photosynthesis’.
16. ECOTOXICITY AND ANALYSIS OF ZNO NANOPARTICLES
IN THE PRESENCE OF MICROORGANISMS.
Photosynthetic activities of (a) Anabaena flos-aquae and
(b) Euglena gracilis after addition of ZnO nanoparticles.
[Zn]=10-3 M.
17. After addition of ZnO, ZnO TOPO, and ZnO-Brij-76
nanoparticles (H = 10), a progressive decrease of
photosynthetic activity was observed in the first 10
days for both microorganisms Anabaena flos-aquae,
after 10 days of incubation, an increase of the
photosynthetic activity was observed after contact
with ZnO and ZnO-Brij-76 nanoparticles. On the other
hand, the presence of ZnO-TOPO nanoparticles
caused cellular death
18. TEM micrographs of Anabaena flos-aquae after contact with
(a) ZnO,
(b) ZnO-TOPO, and
(c) ZnO-Brij-76 nanoparticles.
[Zn] =10-3 M.
19. TEM micrographs of Euglena gracilis after contact with
(a)ZnO,
(b) ZnO-TOPO, and
(c) ZnO-Brij-76 nanoparticles.
20. TIM Micrographs can be developed by using trypan blue
Dye which is a vital stain used to selectively color dead
tissues or cells blue.
Dead cells are shown as a
distinctive blue color under
a photonic microscope
21. CONCLUSIONS
Now the Question is arise what the experiment want to
ask???and why??
o As dicussed earlier,this experiment shows that how different
size,conc.,Hydrolysi ratio effect on living system(microalgae’s)
by intracting with them
o How the toxicity is the variable function of size pf nanoparticles.
o The nature of the biological system can strongly influence the
ecotoxicological results of nanoparticles
22. • Because of the environmental use of nanoparticles and their
unintentional release, the exposure of algae, cyanobacteria,
plants, and fungi to these new materials is a reality cause
eco-toxicity
• A better understanding and assessment of the toxicity and Eco toxicity
of nanoparticles can SAVE ENVIORMENT
23. REFERENCES
1.Gerber. C.,Lang
,H.P.Nat,NANOTECHNOL,2006
2.Gujman ,K.A.D,.Taylor M .R.Banifield Environ
.sci.Techanol 2006
3.Ji.S.L.Ye ,C.H.Jmater .Sci.Techonol 2008
4.Feldmann, C.Adv.Func.Mater 2003
5.Zhang,L,Jiang .Y.Ding.Y.,Povery , M ,.YORK ,D
,J Nanopart.RES 2007
6.Kumar C.S.S.R.,Ed Nanomaterial -toxicity and
enviromental issues Wiley Weinhein 2006
7.Brayner . R .Ferrari –iliou R. Nanotoday 2008.
24. THANK U!!!!!!!
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