a) Since the presenceabsence of the Delta 32 gene is to be detected.pdf
a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5\'-3\' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5\'-3\' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3\'-5\' primer will be complimentary to the 3\'-5\' sequence of the deleted 32 base pair sequence. 3\'-5\' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp. Solution a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5\'-3\' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5\'-3\' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3\'-5\' primer will be complimentary to the 3\'-5\' sequence of the deleted 32 base pair sequence. 3\'-5\' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp..
a) Since the presenceabsence of the Delta 32 gene is to be detected.pdf
a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5\'-3\' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5\'-3\' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3\'-5\' primer will be complimentary to the 3\'-5\' sequence of the deleted 32 base pair sequence. 3\'-5\' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp. Solution a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5\'-3\' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5\'-3\' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3\'-5\' primer will be complimentary to the 3\'-5\' sequence of the deleted 32 base pair sequence. 3\'-5\' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp..
Recomendados
Mais conteúdo relacionado
Mais de anandhomeneeds
Mais de anandhomeneeds(20)
Último
Último(20)
a) Since the presenceabsence of the Delta 32 gene is to be detected.pdf
- 1. a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5'-3' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5'-3' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3'-5' primer will be complimentary to the 3'-5' sequence of the deleted 32 base pair sequence. 3'-5' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp. Solution a) Since the presence/absence of the Delta 32 gene is to be detected, the primers to be used will be complimentary to that gene sequence. The 20-base long 5'-3' primer will be complimentary to the single wildtype DNA sequence corresponding to the deleted 32 base pair sequence as follows: 5'-3' primer: TGTCAGTCAT AGTTAAGAGG The 20-base long 3'-5' primer will be complimentary to the 3'-5' sequence of the deleted 32 base pair sequence. 3'-5' primer: GA CCTTTAAGAA CCTCTTAA b) For a Delta 32/ Delta 32 homozygote, there will be no PCR product generated. This is because in such a genotype, the target DNA will be a deletion, hence primers cannot bind to them. For a Delta 32/+heterozygote: Length of the PCR product can be determined from the nucleotide position of the forward primer and reverse primer. Here, the forward primer will bind to the DNA from position 400 to 420. Reverse primer will bind from position 432 to 412. Hence, the total PCR product size= 432-400= 32 bp.