A genomic sequencing project has discovered the following DNA region in a bacterium that encodes a novel dioxygenase (an enzyme that performs unique oxidation reactions). You as the lead scientist want to obtain a larger quantity of this DNA for transformation studies using a PCR reaction. Below is a single stand of this DNA sequence with two highlighted regions. Your first goal is to design primers that can bind to the target DNA. a) Which of the two regions would be more preferrable for primer design and why? b) Write the sequence of a suitable primer for the above sequence. c) Write the sequence of a potential RNA structure that is complementary to the first highlighted part within above DNA. d) Do the above highlighted DNA regions contain a palindromic sequence? How can you tell? e) If the PCR reaction is successful in production of more DNA, how would you quantify the amount of DNA obtained? Name a suitable analytical technique and the basic work principle..

1. A genomic sequencing project has discovered the following DNA region in a bacterium that
encodes a novel dioxygenase (an enzyme that performs unique oxidation reactions). You as the
lead scientist want to obtain a larger quantity of this DNA for transformation studies using a PCR
reaction. Below is a single stand of this DNA sequence with two highlighted regions. Your first
goal is to design primers that can bind to the target DNA. a) Which of the two regions would be
more preferrable for primer design and why? b) Write the sequence of a suitable primer for the
above sequence. c) Write the sequence of a potential RNA structure that is complementary to the
first highlighted part within above DNA. d) Do the above highlighted DNA regions contain a
palindromic sequence? How can you tell? e) If the PCR reaction is successful in production of
more DNA, how would you quantify the amount of DNA obtained? Name a suitable analytical
technique and the basic work principle.