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Curing AIDS,
with programming
Andrew Grimm
Curing AIDS
Understanding
HIV,
with programming
Andrew Grimm
What's AIDS?
Disease caused by HIV
If untreated, damages the immune system
Damage of immune system can cause death
What’s HIV?
Virus
Cannot live independently
Hijacks the cells of living creatures to reproduce itself
Genome is 9749 letters long
Human genome is 3 billion letters long
Has two copies of the genome
Humans also have two copies of their genome
Impact of AIDS
Prevalence Annual deaths
Worldwide 35 million 1.5 million
Australia 18,000 100
Japan 8,100 100
What am I studying?
Two types of mutation
Point mutation
Recombination
Point mutation
One letter of the genome changing to another letter
Recombination
Swapping equivalent portions of the two copies of the
genome
Why study mutation?
It enables HIV to escape the immune system
It enables HIV to become resistant to drugs
Laboratory component
Start with viruses with a known genome
Infect cells with viruses
Let them reproduce for one generation
Determine the sequence of new viruses
None of this done by me!
Analysis component
Compare sequences against the sequences of the
original
Challenges faced
New questions and
analyses
Questions lead to new analyses
New analyses lead to new insights
New insights lead to new questions
Performance
Initially only dealt with hundreds of sequences
Now deal with millions of sequences
Breaking of assumptions
I used to assume a sequence would be either wild
type or marker type, or a mixture
Now I have to handle wild type or marker type 1 or
marker type 2
Questions?

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