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1. Introduction: Tissue Culture is the in vitro culture of cells, tissues, organs or whole plant under controlled nutritional and environmental Conditions(T. Thorp, 2007). The science of plant tissue culture takes its roots from the discovery of Cells (Robert Hooke in 1665) and propounding of cell theory. In 1838, Schleiden and Schwann proposed that cell is the basic structural unit of all living organisms. They visualized that cell is capable of autonomy and therefore it should be possible for each cell if given an environment to regenerate into whole plants. 2. Plant Tissue Culture: Past & Present Prospects In 1902, a German physiologist, Gottieb Haberlandt for the first time attempted to culture isolated single palisade cells from leaves in knop’s salt solution. The cell remained alive for up to 1 month, increased in size, accumulated starch but failed to divide. Though he was unsuccessful but he laid the foundation of tissue culture so he is regarded as Father of Plant Tissue Culture. In the Subsequent years different landmark discoveries were made. Some of them are: Use of specialized media for aseptic culture of Orchid seeds (Knudson, 1925) and other workers also demonstrated that plants could be propagated in vitro from the minuscule seeds of the Orchidaceae. Further culture of other plant tissue was not possible due to lack of knowledge of the specific hormones to be added to the culture media. This limitation was overcomed by the elucidation of the nature of Auxin, IAA, by Thimann and Went(1930) that plants would be subsequently regenerated through the use of IAA or its analogs. Discovery of Cytokinins, specially Kinetin(6-furfurylaminopurine) by Miller et al. (1956), the regeneration of intact plants from tissue of many herbaceous species became a practical reality.
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