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EFFECT OF STAKING ON
FLOWER INDUCTION,
POLLINATION AND CROSS-
COMPATIBILITY AMONG
SWEETPOTATO CLONES
M.S. AFOLABI, E. E. Carey* and
M.O. Akoroda
University of Ibadan, Nigeria
*CIP c/o CSIR-CRI, Kumasi Ghana
Introduction
•Sweetpotato is ranked 7th in the world
statistics
•Source of Vit. A & minerals
•Free from HCN as in cassava
•Drought tolerance ability
•Good sweetener in industry
•Weed smoldering ability
Limitations of Sweetpotato 
Breeding
•It is highly heterogeneous
• Self- incompatible
• Poor flowering ability
Induction of Flowering in Sweetpotato
Breeding
•Grafting with Ipomoea nil, I. setosa
•Trellises
• Pesticide sprays
•Staking
•Combination of two or more
methods above
Objectives
•Determine the pollination and
cross-compatibility among
sweetpotato clones
•Examine the method for
flower induction
Materials and Methods
•Clones:40
14 Orange flesh
9 white flesh
17 yellow flesh
• Source: Germplasm collection,
Department of Agronomy, University of
Ibadan
•Two Field Experiments: Staking And Non
Staking For Crossing block
•Design: RCBD
NON STAKING PLOT
*The experimental plot consisted of two
rows of 3m long ridge with 1m between
ridges in 3 replications arranged in
Randomized Complete Block Design (RCBD).
*Planting of 25cm long vine cuttings was
done on the crest of each ridge
Each vine cutting was inserted at a slant,
with two-third buried below the soil surface
at a spacing of 30cm
*Other agronomic practices were carried out
except staking
Establishment of Staked plot
Two vines of each clone were
planted on 1 x 1 m ridge with 5
heaps/clone in 3 replications
arranged in Randomized Complete
Block Design (RCBD).
Wooden stakes were erected on
each heap and the vines were tied
with string (to induce flowering)
Covering with 
plastic straw
Rubbing anther on 
stigma
18 clones flowered and were hand‐pollinated
Fruit set after 
hand pollination
Petals tied to avoid 
insect visit
Different flower petal shapes in 
sweetpotato
Pentagonal Shaped
Petal
Semi‐stellate 
Shaped Petal
Round Shaped Petal
Stigma Arrangements in Sweetpotato
Longer Stigma than Anther Same Height as Anther
Stigma Anther
Anther longer than the stigma
Female parent Male parent
Offspring
Example of a crossing
• Total of 306 cross-combinations
expected from all crosses among
the 18 clones
• 109 cross-combinations were
compatible = 35.6% success
Results and Discussion
ᵟ⁄ϙ
199
024.
2
4400
34
BLE
SBO
K
96-
117
1995-
5-21
RE
SIS
TO
EXC
EL
W-
151
440
215
B
A
T
H
SAU
TI
FA
MG
BE 1
440
168
TIS
8250
199
034
.1
AK-
WIDE
TIS
8441
TIS87
/0087
199024.2
N C C C C C C C N N N N N N C N N N
440034
C N C C C C C C N N N N N N C N N N
Blesbok
C C N C C C C C N N N N N N C N N N
96-117
C C C N C C C N N N N N N N C N N N
Resisto
C C C N N N C N N N N N N N C N N N
Excel
C C C C C C N C N N N N N N C N N N
W-151 N N N N N N N N C C C N C C N N C C
440215 N N N N N N N C N C C C N N N C C C
Genetic Compatibility of I8 Sweetpotato Clones
N/B:N=NOT-COMPATIBLE, C=COMPATIBLE
Cloness
Compa-
tibility %
Germi-
nation %
Resisto 16.7 43.3
Sauti 25.0 45.0
Ak-wide 26.7 70.8
TIS 8250 27.5 19.5
199024.2 31.3 67.0
96-117 34.0 44.1
TIS 8441 39.8 83.8
Excel 44.0 31.8
440215 46.3 67.8
1995-5-21 46.4 19.2
% Compatibility and Germination of Sweetpotato Clones
Cloness
Compa-
tibility %
Germi-
nation %
440168 47.5 24.7
Famgbe 1 49.5 26.6
Blesbok 52.9 50.0
Bath 53.3 40.6
199034.1 54.0 64.8
440034 67.1 55.6
TIS
87/0087 67.5 72.2
W-151 68.7 83.3
Total 798.2 910.1
Mean 44.3 50.6
Standard
Deviation 15.3 19.7
CV 34.5 38.9
Clone
Gene.
Comp Rank
Resisto 7.2 17
Sauti 11.3 15
Ak-wide 18.9 10
TIS 8250 5.4 18
199024.2 21.0 9
96-117 15.0 11
TIS 8441 33.4 5
Excel 14.0 12
440215 31.4 6
1995-5-
21 8.9 16
Clone
Gene. 
Comp Rank
440168 11.7 14
Famgbe 1 13.2 13
Blesbok  26.5 7
Bath  21.6 6
199034.1 35.0 4
440034 37.3 3
TIS 
87/0087  67.5 2
W‐151  68.7 1
Genetic Compatibility of Sweetpotato Clones
1. 18 of 40 clones studied produced
flowers and viable seeds
2. Clone W-151 and TIS 87/0087 were
recommended for many cross-
combinations during sweetpotato
breeding.
Conclusions and Recommendations
Acknowledgments
This study was made possible through 
the support of: Centro Internacional de la 
Papa (CIP) Kumasi Ghana by Dr. Ted Carey 
and supervision by  Prof. M.O. Akoroda
and the Department of Agronomy Family 
of the University of Ibadan.
Thank You All For
Listening

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Sess08 1 afolabi effect of staking on flower induction, pollination & cross-compatibility among sweetpotato clones