1. You performed a wheat germ acid phosphatase enzyme assay at 37C for 15 minutes using Citrate
buffer, pH4.8. The data obtained is given in the Table 2 below. 1. Complete the Table 2 . 2. Plot a
double reciprocal plot (Lineweaver Burke) and a Michaelis-Menten graph. Remember to label axis
and include a title and appropriate units. 3. Calculate apparent Km ( y axis) and Vmax using both
plots. Show Km and Vmax values on each of the graph. 4. Attach both Michaelis-Menten and
Lineweaver-Burke plot along with Standard curve to the assignment. 5. Explain how are the Km
and Vmax values calculated from Michaelis Menten plot different from Lineweaver-Burke plot?
Which of the two is more accurate in your opinion and why? 6. Show work for full points. Table 1:
pNP Std. Curve Table 2: Wheat germ acid phosphatase assay