PEF and PurePulse for dummies - part 1

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TOP Technology Talks (TOP b.v.)

Short and simple explanation of PEF

Business Health & Medicine

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
PurePulse for Dummies - 1
Background of cell perforation using PEF
Myriam Knopf
Application Technologist PEF
www.top-bv.nl
www.toptechnologytalks.nl
www.tophealthfacts.nl
www.topfoodlab.nl
www.topwiki.nl

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Background – freshly squeezed, tasty & healthy

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Unfortunnaly microbes are present in the juice …

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
And if we do nothing, exponential growth occurs

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Even outbreaks due to pathogens may occur …
Pathogeen Jaar Aantal zieken Aantal doden Oorzaak Land
Salmonella
Typhimurium
1944 18 1 Restaurant medewerker
(zelf geen symptomen)
Cleveland, Ohio, US
Enterogene E. coli 1992 6 0 Slechte hygiëne
verkopers
India
Salmonella Hartford,
Gaminara, Rubislaw
1995 63, 7 opgenomen
ziekenhuis
0 Amfibieën (padden) Orlando, Florida, US
Shigella flexneri 1995 14 - geen verdere details Zuid-Afrika
Salmonella enterica 1999 500 0 Sinaasappels besmet Adelaide, Australië
Salmonella muenchen 1999 423 1 Mexicaans
sinaasappelsap
Tempe, Arizona, US
Salmonella Anatum 1999 6 0 Straat verkoper, slechte
hygiëne.
Florida, US
Salmonella
Typhimurium
1999 405 0 Pathogeen ongeopende
verpakkingen
Australie
Salmonella enteritidis 2000 74 0 Bron is niet bekend. Calfornia, US
Salmonella
Typhimurium en
SaintPaul
2005 152 - HACCP slecht
gehandhaaft
Meerdere staten, US
Salmonella Panama 2008 15 0 Besmet vruchtensap Nederland

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
But, suppose we put a penlight on the juice …
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ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
… that provides an electric field distribution …
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1,5V

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
.. due to this E-field, the membrane perforates ..
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1,5V

ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
.. and the microbes viability is gone immediately
R.I.P.
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ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
The result? Healthy juice with sufficient shelf-life
21-60 days

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PEF and PurePulse for dummies - part 1

1. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 PurePulse for Dummies - 1 Background of cell perforation using PEF Myriam Knopf Application Technologist PEF www.top-bv.nl www.toptechnologytalks.nl www.tophealthfacts.nl www.topfoodlab.nl www.topwiki.nl

5. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 Even outbreaks due to pathogens may occur … Pathogeen Jaar Aantal zieken Aantal doden Oorzaak Land Salmonella Typhimurium 1944 18 1 Restaurant medewerker (zelf geen symptomen) Cleveland, Ohio, US Enterogene E. coli 1992 6 0 Slechte hygiëne verkopers India Salmonella Hartford, Gaminara, Rubislaw 1995 63, 7 opgenomen ziekenhuis 0 Amfibieën (padden) Orlando, Florida, US Shigella flexneri 1995 14 - geen verdere details Zuid-Afrika Salmonella enterica 1999 500 0 Sinaasappels besmet Adelaide, Australië Salmonella muenchen 1999 423 1 Mexicaans sinaasappelsap Tempe, Arizona, US Salmonella Anatum 1999 6 0 Straat verkoper, slechte hygiëne. Florida, US Salmonella Typhimurium 1999 405 0 Pathogeen ongeopende verpakkingen Australie Salmonella enteritidis 2000 74 0 Bron is niet bekend. Calfornia, US Salmonella Typhimurium en SaintPaul 2005 152 - HACCP slecht gehandhaaft Meerdere staten, US Salmonella Panama 2008 15 0 Besmet vruchtensap Nederland

PEF and PurePulse for dummies - part 1

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