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EFFOST2015_0552-MIRON-POSTER

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EFFOST2015_0552-MIRON-POSTER

  1. 1. The first handmade computer-stirred tank bioreactor, using an agitator-converted AMD ventilator for plant cell and microorganism cultivation. Abstract: In recent years, a couple of bioreactor modifications have been developed and successfully adopted for the in vitro cultivation of plant cells and microorganisms. Bioreactors such as mechanically agitated, airlift and photobioreactors have been designed and used for large scale cultivation of algae, higher biomass and bioactive compounds production. Our primary objective of this study was to build and design a cost effective and ecofriendly stirred tank bioreactor 4L, where the agitation rate (i.e. 150, 250, 500 rpm) would be controlled through computer software without affecting the personal computer performance (temperature, graphics). As a result we have designed and developed the first computer in the world that is capable to yield more than a simple computer (fermentation). This project aims to help every person in the world, who has access to a desktop computer, to accomplish similar achievements. Furthermore, due to our friendly stance towards environment, we have manufactured our computer tower, using Plexiglas, a material easy to break down into its original components and completely recycled. Key words: AMD ventilator, stirred tank bioreactor, computer, bioactive compounds, computer software, Plexiglass, ecofriendly. 1. INTRODUCTION: The concept of continuous culture dates back to 19th century, when a continuous process for the conversion of waste beers and wines to vinegar was developed. The chemostat invented in the early ‘40's marked the advent of serious continuous fermentation. The use of continuous stirred tank reactor (CSTR) to extend duration of culture of microbes was developed in the ‘50s by Novick, Szilard and Monod. The realization that a CSTR could be used to maintain microbial growth at a steady state value, that could be varied from any growth rate was an important advance. It surpassed the traditional thinking at a time when stable microbial growth was only possible at the maximum rate, corresponding to the minimum doubling time found in batch cultures. Bioreactors due to presence of mechanical pumps require high power consumption. As a result in the 21st century, we are able to introduce the most useful tool, a computer- fermenter without additional energy costs. 4. DISCUSSION: Some of the bioreactor’s pros: Extremely compact than any bioreactor system on the market e.g 100 Liter QVF glass portable used agitated tanks cost 2276.66 Euro, in contrast to our bioreactor, which costs about 20 Euro (excluding the motherboard). Despite of its compact size, the bioreactor allows a perfect visible access from all sides! Does not require additional energy, because the primary circuit has been converted, without affecting computer performance. Weighs only 6 kg. It is portable. It is recyclable. 5. REFERENCES: 1) Agarwal, P., and Lim, H.C. (1984) Analyses of various control schemes for continuous bioreactors. Advances In Biochemical Engineering/Biotechnology, 30, 61-90. 2) Miller, Paul (2006-07-08). "Apple sneaks new logic board into whining MacBook Pros". Engadget. Retrieved 2013-10-30 3) Mueller, Scott 2005. Upgrading and Repairing PCs. Que Publishing. 16th edition. pp 1274–1280 4) Arpaci-Dusseau, Remzi H.; Arpaci-Dusseau, Andrea C. (2014). "Operating Systems: Three Easy Pieces [Chapter: Hard Disk Drives]" Arpaci-Dusseau Books. 5) "SpeedFan - Access temperature sensor in your computer. Almico.com 6) Encyclopedia of Packaging Technology, Third Edition by Kit L. Yam 2. METHOD AND MATERIALS 1.1.Motherboard Asus 2V- 1.2.Ventilator 1.3.HDD seagate 1.4.SpeedFan 4.51 1.5. Stainless steel coil 1.6.Glass Jar 3. DEFINITIONS: 1.1. Motherboard is one of the most essential parts of a computer system. It holds together many of the crucial components of a computer, including the central processing unit (CPU), memory and connectors for input and output devices. 1.2. CPU fan. Used to cool the CPU (central processing unit) heatsink. Effective cooling of a concentrated heat source such as a large-scale integrated circuit requires a heatsink, which may be cooled by a fan; use of a fan alone will not prevent overheating of the small chip. 1.3. HDD. A hard disk drive is a data storage device used for storing and retrieving digital information using one or more rigid ("hard") rapidly rotating disks (platters) coated with magnetic material. 1.4. SpeedFan is a program that monitors voltages, fan speeds and temperatures in computers with hardware monitor chips. 1.5. Stainless steel coil, a form of steel coil, containing chromium, resistant to tarnishing and rust. 1.6. Glass Jar 4L. . Preparation and sterilization of culture media. 15 psi, 121° C for 15 Inoculate with preferable microorganism in laminar air flow chamber to avoid contamination. • CCP2 Power on the computer to manipulate agitation rate through SpeedFan software • CCP3 F L O W C H A R T Main window A. Miron2, G. Kuziortis2, and T.P. Zafiriadis1 1 DVM-Food Technologist MSc, Professor of Katerini’s Public Educational Training Institution, Director of ESTIA LAB IKE 2 Undergraduate Students, Technological Educational Institution of Thessaly Contact: arismirons@gmail.com • CCP1

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