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RESUME
RESUME
rakesh jangra
Hcdh b niang_ministre_008
Hcdh b niang_ministre_008
causepremiere
Endothelial cells , the lining cells of blood vessels , are involved in many physiological and pathophysiological process, including haemostasis, vasoregulation, inflammation, angiogenesis, and the extravasation of fluids, macromolecules, hormones and leucocytes. This work was carried out to investigate the isolation, culture and characterization of human umbilical vein endothelial cells (HUVECs) in vitro by the help of Flow cytometry and Immunocytochemistry. Moreover, protocol was standardized to improve the cell yield and growth of these cells in culture. The endothelial cells were isolated by collagenase digestion and were seeded in tissue culture flasks using Dulbeccos modified Eagles medium (DMEM) –LG(Low Glucose) and M-200 media specific for ECGM (Endothelial Cell Growth Media ) containing LSGS( Low Serum Growth Supplement) and growth factor containing the (VEGF) and (bFGF) This study showed that HUVECS attached to to the gelatin coated tissue culture plates and become confluent after 3 days in vitro .HUVECs displayed cobblestone morphology at confluence with granular cytoplasm. HUVECs were positive for CD31- 3 % positivity, VE-Cadherin - 18.8% and vWF- 30.3%. Immunocytochemistry helped to anatomically a visualize the localization of endothelial cells marker. The cells produced from this technique grew well for extend periods and retained the normal characteristics of primary HUVECs, making them invaluable tools for researchers. Keywords: HUVECs., Flow Cytometry, Immunocytochemistry,VE-Cadherin,Vwf
Isolation of Human Umbilical Vein Endothelial Cells (HUVECs) from Umbilical C...
Isolation of Human Umbilical Vein Endothelial Cells (HUVECs) from Umbilical C...
22rohit
Este es mi trabajo
Métodos anticonceptivos y enfermedades de transmisión sexual
Métodos anticonceptivos y enfermedades de transmisión sexual
SANTIAGO GIRALDO VALLEJO
Made this with a couple of my friends in 2015. It was to be submitted by Prakruti Kodali (ISB '16) for the Matrix Million Dollar B-Plan Elevator Pitch.
Matrix million dollar pitch channel project baby blue
Matrix million dollar pitch channel project baby blue
Tejaswi Subramanian
7 precios
7 precios
estherpena
TABLA DE POSICIONES ARFA SUB - 17 2012 GRUPO 1 Y 2 ZONA A LITORAL NORTE.
Tabla de posiciones arfa sub 17 2012 grupo 1 y 2 zona a litoral norte
Tabla de posiciones arfa sub 17 2012 grupo 1 y 2 zona a litoral norte
Itv Petorca
actividades de la unidad 4
U4 ecommerce
U4 ecommerce
bianca zamora
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RESUME
RESUME
rakesh jangra
Hcdh b niang_ministre_008
Hcdh b niang_ministre_008
causepremiere
Endothelial cells , the lining cells of blood vessels , are involved in many physiological and pathophysiological process, including haemostasis, vasoregulation, inflammation, angiogenesis, and the extravasation of fluids, macromolecules, hormones and leucocytes. This work was carried out to investigate the isolation, culture and characterization of human umbilical vein endothelial cells (HUVECs) in vitro by the help of Flow cytometry and Immunocytochemistry. Moreover, protocol was standardized to improve the cell yield and growth of these cells in culture. The endothelial cells were isolated by collagenase digestion and were seeded in tissue culture flasks using Dulbeccos modified Eagles medium (DMEM) –LG(Low Glucose) and M-200 media specific for ECGM (Endothelial Cell Growth Media ) containing LSGS( Low Serum Growth Supplement) and growth factor containing the (VEGF) and (bFGF) This study showed that HUVECS attached to to the gelatin coated tissue culture plates and become confluent after 3 days in vitro .HUVECs displayed cobblestone morphology at confluence with granular cytoplasm. HUVECs were positive for CD31- 3 % positivity, VE-Cadherin - 18.8% and vWF- 30.3%. Immunocytochemistry helped to anatomically a visualize the localization of endothelial cells marker. The cells produced from this technique grew well for extend periods and retained the normal characteristics of primary HUVECs, making them invaluable tools for researchers. Keywords: HUVECs., Flow Cytometry, Immunocytochemistry,VE-Cadherin,Vwf
Isolation of Human Umbilical Vein Endothelial Cells (HUVECs) from Umbilical C...
Isolation of Human Umbilical Vein Endothelial Cells (HUVECs) from Umbilical C...
22rohit
Este es mi trabajo
Métodos anticonceptivos y enfermedades de transmisión sexual
Métodos anticonceptivos y enfermedades de transmisión sexual
SANTIAGO GIRALDO VALLEJO
Made this with a couple of my friends in 2015. It was to be submitted by Prakruti Kodali (ISB '16) for the Matrix Million Dollar B-Plan Elevator Pitch.
Matrix million dollar pitch channel project baby blue
Matrix million dollar pitch channel project baby blue
Tejaswi Subramanian
7 precios
7 precios
estherpena
TABLA DE POSICIONES ARFA SUB - 17 2012 GRUPO 1 Y 2 ZONA A LITORAL NORTE.
Tabla de posiciones arfa sub 17 2012 grupo 1 y 2 zona a litoral norte
Tabla de posiciones arfa sub 17 2012 grupo 1 y 2 zona a litoral norte
Itv Petorca
actividades de la unidad 4
U4 ecommerce
U4 ecommerce
bianca zamora
Conprobantes de comtabilidad o soporte
Conprobantes de comtabilidad o soporte
Natalia-karen
Presentación contaminacion
Presentación contaminacion
Leslie Pichardo
Resume_src
Resume_src
Yiyi Zhang
The learning process and its outcomes depend greatly on the social interaction between teachers and students and, in particular, on the proficient and focused use of language through written text or discussions. Our overarching goal in this project is to better understand how to make automated tutorial dialogues effective and adaptive to student characteristics, such as prior knowledge. The specific goal of our current project is to develop an adaptive, natural-language tutoring system, driven by a student model, which can effectively carry out reflective conversations with students after they solve physics problems. Towards this end, we continue our work in identifying linguistic features of tutoring that predict learning gains, and extend it by characterizing the “level of support” to provide to students based on their current level of understanding particular physics concepts and principles, as dynamically captured by the student model. In this talk, I will describe the features of dialogic discourse underlying “level of support” that we have identified through the analysis of human-to-human tutorial dialogues as well as the construction and application of a coding scheme for the characterization of the “level of support”. I will present initial teacher feedback on dialogues that apply these features to coach students at different levels. I will also discuss how this line of research affects the authoring of tutorial dialogues used by an intelligent tutoring system for students who exhibit different levels of understanding.
Chounta Talk @HCII: Linking Dialogue with Student Modeling to Create an Enhan...
Chounta Talk @HCII: Linking Dialogue with Student Modeling to Create an Enhan...
Irene-Angelica Chounta
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Conceptos sobre algoritmos
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For A Perfect Photo Acesse o site: www.rsnoticias.net
For A Perfect Photo
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7 steps How to prevent Thalassemia
7 steps How to prevent Thalassemia : Dr Sharda Jain & Vandana Gupta
7 steps How to prevent Thalassemia : Dr Sharda Jain & Vandana Gupta
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These are simplified slides discussing the regulation of cardiac output and venous return. Learning objectives: 1. Comprehend the determinants of cardiac output and factors affecting cardiac output 2. Comprehend the factors affecting stroke volume and heart rate and total peripheral resistance 3. Identify the factors regulating venous return 4. Discuss the causes of high and low output cardiac failure 5. Enlist the functions of veins and recognise the significance of venous reservoirs Study Resources: 1. Chapter 20, Guyton and Hall Textbook of Medical Physiology, 14th edition 2. Chapter 30 and 32, Ganong’s Review of Medical Physiology, 26th edition 3. Chapter 10, Human Physiology by Lauralee Sherwood, 9th edition 4. Physiology, Cardiac Output - StatPearls https://www.ncbi.nlm.nih.gov/books/NBK470455/
Cardiac Output, Venous Return, and Their Regulation
Cardiac Output, Venous Return, and Their Regulation
MedicoseAcademics
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Explore the fundamentals of the human reproductive system in this concise presentation, suitable for medical students and professionals alike. Covering anatomy, physiology, and Pregnancy, it offers essential knowledge for understanding reproductive health.
ANATOMY AND PHYSIOLOGY OF REPRODUCTIVE SYSTEM.pptx
ANATOMY AND PHYSIOLOGY OF REPRODUCTIVE SYSTEM.pptx
Swetaba Besh
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In this lecture on circulatory shock, Dr. Faiza, an Assistant Professor of Physiology, delves into the profound implications of inadequate blood flow throughout the body, leading to tissue damage. The session begins by defining circulatory shock and elucidating its physiological causes, including decreased cardiac output, diminished blood volume, decreased vascular tone, obstruction to blood flow, excess metabolic rate, and abnormal perfusion patterns. Dr. Faiza categorizes shock into various types, such as cardiogenic shock, hypovolemic shock, neurogenic shock, obstructive shock, anaphylactic shock, and septic shock, and explores the pathophysiological basis of each. Furthermore, the lecture examines the stages of shock, from the non-progressive phase where compensatory mechanisms aim for full recovery to the progressive phase where shock worsens steadily without intervention, potentially leading to irreversible damage. Dr. Faiza discusses therapeutic interventions for shock, including replacement therapy, blood and plasma transfusion, sympathomimetic drugs, and other adjunctive treatments like head-down positioning, oxygen therapy, and glucocorticoids.
Circulatory Shock, types and stages, compensatory mechanisms
Circulatory Shock, types and stages, compensatory mechanisms
MedicoseAcademics
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The learning process and its outcomes depend greatly on the social interaction between teachers and students and, in particular, on the proficient and focused use of language through written text or discussions. Our overarching goal in this project is to better understand how to make automated tutorial dialogues effective and adaptive to student characteristics, such as prior knowledge. The specific goal of our current project is to develop an adaptive, natural-language tutoring system, driven by a student model, which can effectively carry out reflective conversations with students after they solve physics problems. Towards this end, we continue our work in identifying linguistic features of tutoring that predict learning gains, and extend it by characterizing the “level of support” to provide to students based on their current level of understanding particular physics concepts and principles, as dynamically captured by the student model. In this talk, I will describe the features of dialogic discourse underlying “level of support” that we have identified through the analysis of human-to-human tutorial dialogues as well as the construction and application of a coding scheme for the characterization of the “level of support”. I will present initial teacher feedback on dialogues that apply these features to coach students at different levels. I will also discuss how this line of research affects the authoring of tutorial dialogues used by an intelligent tutoring system for students who exhibit different levels of understanding.
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For A Perfect Photo Acesse o site: www.rsnoticias.net
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