Innovative yeast biofraction for a more radiant look Brighter, evenly toned skin is often an element of a fresh, rejuvenated appearance, and the pursuit of this has been ongoing for centuries. Ancient Egyptians applied an ointment of alabaster and donkey milk to lighten their skin. The Romans whitened their complexion using chalk or white lead, and during The Renaissance, women pursued a transparent complexion. Today, consumers continue to look for ways to brighten their skin. To meet this need, ISP developed Natriance™ Brightener, a biofraction derived from yeast (Saccharomyces) that helps brighten the appearance of skin. Rich in essential amino acids and small peptides, which have strong nutritive properties, it provides an advantage over commonly used brightening technologies. Skin color is typically determined by the amounts of melanin pigment produced in the epidermis. As consumers age, melanocytes, which make the pigment, function more erratically, leading to overproduction or uneven production that results in dark spots on the skin. To battle this phenomenon, consumers have had cosmetic options, some of which may not be ideal. Unlike other available cosmetic methods such as laser and peel treatments, which can be highly expensive and create discomfort, Natriance Brightener is a patent-pending ingredient for use in mass market products to brighten the appearance of skin. Applications • Radiance enhancing products • Skin brighteners • Skin color unifying products • Age spot products • Anti-aging products • Hand care products

1. Natriance™Brightener
Biofraction designed to help brighten
the appearance of skin
Features and Benefits
Helps brighten and even skin tone
Contains beneficial amino acids that have nutritive properties
Ecocert compliant
Derived from a natural ingredient (yeast)
I
PERSONAL CARE
Innovative yeast biofraction for a more radiant look
Brighter, evenly toned skin is often an element of a fresh, rejuvenated
appearance, and the pursuit of this has been ongoing for centuries.
Ancient Egyptians applied an ointment of alabaster and donkey milk to
lighten their skin. The Romans whitened their complexion using chalk or
white lead, and during The Renaissance, women pursued a transparent
complexion. Today, consumers continue to look for ways to brighten
their skin.
To meet this need, ISP developed Natriance™ Brightener, a biofraction
derived from yeast (Saccharomyces) that helps brighten the appearance
of skin. Rich in essential amino acids and small peptides, which have
strong nutritive properties, it provides an advantage over commonly
used brightening technologies.
Skin color is typically determined by the amounts of melanin pigment
produced in the epidermis. As consumers age, melanocytes, which make
the pigment, function more erratically, leading to overproduction or
uneven production that results in dark spots on the skin. To battle this
phenomenon, consumers have had cosmetic options, some of which
may not be ideal. Unlike other available cosmetic methods such as
laser and peel treatments, which can be highly expensive and create
discomfort, Natriance Brightener is a patent-pending ingredient for use
in mass market products to brighten the appearance of skin.
Applications
• Radiance enhancing products
• Skin brighteners
• Skin color unifying products
• Age spot products
• Anti-aging products
• Hand care products

2. Ex-vivo testing
Significantly improved efficacy over leading benchmark
In ex-vivo testing, Natriance Brightener has been shown to provide markedly better brightening benefits for skin’s appearance
than standards such as Kojic acid, even when used at lower levels. This superior efficacy demonstrates the effectiveness of the
yeast-based ingredient at helping to brighten and even skin tone.
Efficacy compared to Kojic acid
Compared with a 1% PBS solution of Kojic acid and a placebo, a 0.5% PBS solution of Natriance Brightener yielded visibly brighter results
as revealed by Fontana Masson histological staining of melanin and measured by Image Pro® software.
Quantification of brightening efficacy
Similarly, when measuring the intensity of skin color following the application of Natriance Brightener, it is clear that it provides signifi-
cantly better results than Kojic acid, even at half the concentration.
Control Kojic acid Natriance
Brightener
0.5%
0
40
60
80
100
120
140
160
200
Averageintensity/length
(arbitraryunits)
180
-25.9% -81.2%
20
Natriance
Brightener
1%
-55.8%
** ** ***
Placebo Natriance Brightener 0.5% Kojic Acid 1%
Intensity

3. Clinical study
When tested on a group of Asian volunteers, Natriance
Brightener showed significantly better results than a placebo
(in-vivo). The double-blind test compared a cream containing
3% Natriance Brightener against a placebo over 42 days. Each
of the 20 panelists applied the creams twice a day after using
a sunscreen two hours prior. Skin luminance measurements
were taken on days 0, 14, 28 and 42 using a CM-2600d
spectrophotometer; the L* value of the CIELAB (L.a.b) color
space was recorded to determine lightness of skin.
Within the L.a.b color space, the L* value indicates relative
lightness (luminance) of skin, with higher values denoting a
greater degree of lightness. At each measurement, the mean
value of L*, for the treated skin, was higher than that of the
placebo. By the end of the 42 days, 75% of the panelists showed
improvements in skin luminance.
Time vs skin luminance values
The effect of Natriance Brightener was maximized at day 14,
averaging an L* value increase of 0.35 over the placebo. The benefit
was prolonged with continued application, as 75% of the panelists
experienced improvements in skin brightness.
Conclusion
Based on both ex-vivo and in-vivo testing, Natriance Brightener
helps consumers to brighten the appearance of their skin and
achieve a younger, more radiant and fresher look.
Natriance Brightener is part of the Natriance biofraction family
of mass market ingredients. Designed to promote a youthful,
vibrant look, these ISP innovations enable marketers to deliver
a wide array of benefits to consumers through everyday
products.
Overview
Physical properties
Appearance Limpid solution
Color Yellow
Odor Characteristic
INCI name
Water (and) Glycerin (and) Hydrolyzed Yeast Protein (proposed)
Formulation guidelines
Recommended use level: 3%
Placebo
Natriance Brightener
D14 - D0 D28 - D0 D42 - D0
0
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.45
MeanL*value
0.40
+0.40
Time
+0.31 +0.35

4. I
ispcorp.com
The information contained in this brochure and the various products described are intended for use only by persons having technical skill and at their own discretion and risk after
they have performed necessary technical investigations, tests and evaluations of the products and their uses. This material is for informational purposes only and describes the
scientific support for the use of Natriance™ Brightener as an ingredient in cosmetic products intended to enhance appearance and other cosmetic benefits. While the information
herein is believed to be reliable, we do not guarantee its accuracy and a purchaser must make its own determination of a product’s suitability for purchaser’s use, for the protection of
the environment, and for the health and safety of its employees and the purchasers of its products. Neither ISP nor its affiliates shall be responsible for the use of this information, or
of any product, method, formulation, or apparatus described in this presentation. Nothing herein waives any of ISP’s or its affiliates’ conditions of sale, and WE MAKE NO WARRANTY,
EXPRESS OR IMPLIED, OF MERCHANTABILITY OR FITNESS OF ANY PRODUCT FOR A PARTICULAR USE OR PURPOSE. We also make no warranty against infringement of any patents
by reason of purchaser’s use of any information, product, method or apparatus described in this presentation.
™ Trademark registration applied for ® Registered trademark of the ISP group © International Specialty Products. 2010 Printed in USA.
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