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MLS 211: INTRODUCTION TO MEDICAL
LABORATORY SCIENCE
BY
MLS SHUAIBU B.I
Department of Medical Laboratory Science
Faculty Of Applied Health Sciences
Edo State University, Uzairue
25/10/2022 1
MODULE 1
25/10/2022 2
Learning Outcomes
At the end of this class, students should be able to:
1. Define Medical Laboratory Science and explain its relevance as a discipline.
2. Define Medical Microbiology and list its sub- specialties.
3. Define micro-organism and highlight the differences between Prokaryotes
and Eukaryotes.
4. Differentiate between infection and infectious disease.
5. List and explain the modes of transmission of infectious agents.
25/10/2022 3
INTRODUCTION
Medical Laboratory Science is concerned with the analysis of clinical
specimen such as:
 urine
blood
 stool
peritoneal fluid
synovial fluid e.t.c, with the aim of identifying the cause/s of disease
conditions.
It is pivotal to the effective management of diseases.
 Reports has shown that over 60 percent of decision relating to hospital
admissions, prescribed medicals and discharge of patients depends on
25/10/2022 4
INTROD……………
Medical laboratory science as a discipline is thus a fulcrum for practice of
modern day medicine.
One trained to perform the function above is regarded as a Medical
Laboratory Scientist or a Clinical Laboratory Scientist.
25/10/2022 5
INTROD……………
Medical laboratory (also called Clinical laboratory) is a facility that
provides controlled conditions in which tests are done on clinical
specimens in order to acquire information about the health of an
individual (or patient) for the purpose of:
Diagnosis
Treatment
 Prevention of disease or medical research.
25/10/2022 6
Specialties in Medical Laboratory Science
Medical Laboratory Science has many specialties.
They include:
Medical Microbiology
Chemical Pathology
Haematology and Blood transfusion
Histopathology and
Immunology.
Different specialists work together providing valuable data for management of
25/10/2022 7
Medical Microbiology
Medical Microbiology is the study of micro-organisms, the diseases they cause and
host response to these diseases.
It is a branch of medical sciences that deals with the etiology, pathogenesis,
laboratory diagnosis, treatment and control of infection.
Micro-organisms are small (microscopic) organism that may exist in single or
multicellular form.
They are very small organism that cannot be seen with the naked eyes except with
25/10/2022 8
Medical Microbiology……………
Micro-organisms are ubiquitous and can be found everywhere and
anywhere.
They can be found in the air, soil, water, animals, and man.
Many micro-organisms are known to cause disease conditions in man.
Micro-organisms that can cause diseases are termed pathogens.
Based on cellular structure, all cells including microbes are divided into two
groups, namely:
25/10/2022 9
Medical Microbiology Laboratory
This laboratory deals with the study of human pathogens-Pathogens are
biological agents that cause diseases to their hosts.
They include microorganisms such as:
Bacteria
Viruses
 Fungi)
Parasites (e.g. intestinal worms, lice and malaria parasites) of medical
25/10/2022 10
SUB- SPECIALTIES OF MEDICAL MICROBIOLOGY
These sub-specialties are:
1. MedicalVirology: Focuses on the study of viruses and the diseases they
cause in man.
2. Medical Bacteriology: Focuses on the study of bacteria and the diseases
they cause in man
3. Medical Mycology: This focuses on the study of fungi that causes disease
in man.
4. Medical Parasitology: Focuses on the study of parasitic diseases in man
25/10/2022 11
INFECTION AND INFECTIOUS DISEASE
Infection is the invasion of a tissue by micro-organisms (bacteria, viruses,
fungi etc).
An infectious disease is a disorder resulting from the invasion and
colonization of tissue by micro-organism (bacteria, fungi, viruses etc).
One with an infectious disease typically presents with signs and symptoms
of illness.
25/10/2022 12
Cont.……
Based on place of acquisition, infection can be classified as
1. Nosocomial infection. This is sometimes called Health-care associated
infection.
This is an infection that is acquired in a hospital or any other health care
facility such as clinics, maternity homes, rehabilitation homes etc.
Infection can be spread to a susceptible host in a clinical setting through
contaminated equipment, beddings, health care staff, another patient, or
even in some cases from the patient’s own microbiota, particularly after
surgery.
2. Community acquired infection: This is any infection acquired outside a
25/10/2022 13
MODE OF TRANSMISION OF INFECTIOUS AGENTS
There are a number of ways through which infectious agents can be
transmitted to cause disease. These are;
1. Human to human contact: This involves direct body contact with that of
an infected person leading to transfer of infective agent.
2. Feacal oral route: This involves the ingestion of materials (food, water)
that have been contaminated with infective micro-organisms.
3. Vertical transmission: This refers to placenta transfer of infectious agents
from mother to child.
25/10/2022 14
Cont.…………
4. Aerosol: Transmission can be through infective airborne droplets.
Droplets are formed from infected persons during actions like coughing ,
sneezing and talking.
Inhalation of these droplets that contain infective micro-organism by
susceptible host can cause infection.
5. Vector borne transmission: This occurs when certain vectors such as
mosquitoes, rats, fly etc, transmit infective micro-organism to susceptible
host.
25/10/2022 15
DIAGNOSIS OF INFECTIOUS DISEASES
Effective management of infectious diseases depends largely on the timely and
accurate diagnosis of its etiologic agents. This can be achieved by the use of
one or a combination of the following laboratory methods
Microscopy: This involves the use of microscope in viewing clinical specimen
in other to reveal the presence of inherent micro-organism that may be the
cause of disease.
Serological Technique: This relates to procedures that detects specific
antigens or antibodies of pathogens in clinical specimens of patients.
Cultural method: This employs the use of appropriate culture media in
growing and identifying the pathogen in the laboratory.
25/10/2022 16
Cont.…………..
Biochemical tests:
These are tests used to confirm the identity of pathogens in the Laboratory.
Laboratory.
Common biochemical test used in the laboratory include citrate utilization
test, coagulase, catalase, indole tests, urease test, oxidase tests and vogue-
proskauer tests.
Nucleic acid detection (Polymerase Chain Reaction):
This is an amplification technique that allows the detection and selective
replication of a targeted portion of a genome.
Basically, the procedure sets out to amplify small quantity of microbes into
several million copies, followed by the detection of nucleic acid on a
specialized medium.
25/10/2022 17
• Haematology: This laboratory is involved in the performance of
relevant tests (on blood) in the diagnosis of blood diseases, (e.g.
Anaemia, Haemoglobinopathies, Leukaemia etc) and blood
transfusion services e.g. blood group, blood cross matching.
25/10/2022 18
• Chemical Pathology Laboratory (Clinical Chemistry or Clinical
Biochemistry Laboratory): This division of laboratory is concerned
with the performance of quantitative and qualitative tests on clinical
specimens to investigate the state of various body chemistries.
• Such clinical specimens include body fluids (e.g. whole blood, plasma,
serum, urine, sweat, cerebrospinal fluid) and occasionally faeces,
tissue, hair e.t.c.
25/10/2022 19
• Histopathology Laboratory: This is the laboratory where tissues (or
cells) are processed for microscopic examination in order to
investigate or study disease manifestations on the tissue (or cells),
structure, for diagnostic purposes e.g. Cancer diagnosis.
• In the laboratory, tissue samples are processed onto glass slides from
which effects of diseases on the histological architecture of tissues
can be microscopically examined and hence diagnostic inferences are
made e.g. Cancer diagnosis.
25/10/2022 20
THANK YOU
?
25/10/2022 21
MODULE 2
25/10/2022 22

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MLS 211 PART I.pptx

  • 1. MLS 211: INTRODUCTION TO MEDICAL LABORATORY SCIENCE BY MLS SHUAIBU B.I Department of Medical Laboratory Science Faculty Of Applied Health Sciences Edo State University, Uzairue 25/10/2022 1
  • 3. Learning Outcomes At the end of this class, students should be able to: 1. Define Medical Laboratory Science and explain its relevance as a discipline. 2. Define Medical Microbiology and list its sub- specialties. 3. Define micro-organism and highlight the differences between Prokaryotes and Eukaryotes. 4. Differentiate between infection and infectious disease. 5. List and explain the modes of transmission of infectious agents. 25/10/2022 3
  • 4. INTRODUCTION Medical Laboratory Science is concerned with the analysis of clinical specimen such as:  urine blood  stool peritoneal fluid synovial fluid e.t.c, with the aim of identifying the cause/s of disease conditions. It is pivotal to the effective management of diseases.  Reports has shown that over 60 percent of decision relating to hospital admissions, prescribed medicals and discharge of patients depends on 25/10/2022 4
  • 5. INTROD…………… Medical laboratory science as a discipline is thus a fulcrum for practice of modern day medicine. One trained to perform the function above is regarded as a Medical Laboratory Scientist or a Clinical Laboratory Scientist. 25/10/2022 5
  • 6. INTROD…………… Medical laboratory (also called Clinical laboratory) is a facility that provides controlled conditions in which tests are done on clinical specimens in order to acquire information about the health of an individual (or patient) for the purpose of: Diagnosis Treatment  Prevention of disease or medical research. 25/10/2022 6
  • 7. Specialties in Medical Laboratory Science Medical Laboratory Science has many specialties. They include: Medical Microbiology Chemical Pathology Haematology and Blood transfusion Histopathology and Immunology. Different specialists work together providing valuable data for management of 25/10/2022 7
  • 8. Medical Microbiology Medical Microbiology is the study of micro-organisms, the diseases they cause and host response to these diseases. It is a branch of medical sciences that deals with the etiology, pathogenesis, laboratory diagnosis, treatment and control of infection. Micro-organisms are small (microscopic) organism that may exist in single or multicellular form. They are very small organism that cannot be seen with the naked eyes except with 25/10/2022 8
  • 9. Medical Microbiology…………… Micro-organisms are ubiquitous and can be found everywhere and anywhere. They can be found in the air, soil, water, animals, and man. Many micro-organisms are known to cause disease conditions in man. Micro-organisms that can cause diseases are termed pathogens. Based on cellular structure, all cells including microbes are divided into two groups, namely: 25/10/2022 9
  • 10. Medical Microbiology Laboratory This laboratory deals with the study of human pathogens-Pathogens are biological agents that cause diseases to their hosts. They include microorganisms such as: Bacteria Viruses  Fungi) Parasites (e.g. intestinal worms, lice and malaria parasites) of medical 25/10/2022 10
  • 11. SUB- SPECIALTIES OF MEDICAL MICROBIOLOGY These sub-specialties are: 1. MedicalVirology: Focuses on the study of viruses and the diseases they cause in man. 2. Medical Bacteriology: Focuses on the study of bacteria and the diseases they cause in man 3. Medical Mycology: This focuses on the study of fungi that causes disease in man. 4. Medical Parasitology: Focuses on the study of parasitic diseases in man 25/10/2022 11
  • 12. INFECTION AND INFECTIOUS DISEASE Infection is the invasion of a tissue by micro-organisms (bacteria, viruses, fungi etc). An infectious disease is a disorder resulting from the invasion and colonization of tissue by micro-organism (bacteria, fungi, viruses etc). One with an infectious disease typically presents with signs and symptoms of illness. 25/10/2022 12
  • 13. Cont.…… Based on place of acquisition, infection can be classified as 1. Nosocomial infection. This is sometimes called Health-care associated infection. This is an infection that is acquired in a hospital or any other health care facility such as clinics, maternity homes, rehabilitation homes etc. Infection can be spread to a susceptible host in a clinical setting through contaminated equipment, beddings, health care staff, another patient, or even in some cases from the patient’s own microbiota, particularly after surgery. 2. Community acquired infection: This is any infection acquired outside a 25/10/2022 13
  • 14. MODE OF TRANSMISION OF INFECTIOUS AGENTS There are a number of ways through which infectious agents can be transmitted to cause disease. These are; 1. Human to human contact: This involves direct body contact with that of an infected person leading to transfer of infective agent. 2. Feacal oral route: This involves the ingestion of materials (food, water) that have been contaminated with infective micro-organisms. 3. Vertical transmission: This refers to placenta transfer of infectious agents from mother to child. 25/10/2022 14
  • 15. Cont.………… 4. Aerosol: Transmission can be through infective airborne droplets. Droplets are formed from infected persons during actions like coughing , sneezing and talking. Inhalation of these droplets that contain infective micro-organism by susceptible host can cause infection. 5. Vector borne transmission: This occurs when certain vectors such as mosquitoes, rats, fly etc, transmit infective micro-organism to susceptible host. 25/10/2022 15
  • 16. DIAGNOSIS OF INFECTIOUS DISEASES Effective management of infectious diseases depends largely on the timely and accurate diagnosis of its etiologic agents. This can be achieved by the use of one or a combination of the following laboratory methods Microscopy: This involves the use of microscope in viewing clinical specimen in other to reveal the presence of inherent micro-organism that may be the cause of disease. Serological Technique: This relates to procedures that detects specific antigens or antibodies of pathogens in clinical specimens of patients. Cultural method: This employs the use of appropriate culture media in growing and identifying the pathogen in the laboratory. 25/10/2022 16
  • 17. Cont.………….. Biochemical tests: These are tests used to confirm the identity of pathogens in the Laboratory. Laboratory. Common biochemical test used in the laboratory include citrate utilization test, coagulase, catalase, indole tests, urease test, oxidase tests and vogue- proskauer tests. Nucleic acid detection (Polymerase Chain Reaction): This is an amplification technique that allows the detection and selective replication of a targeted portion of a genome. Basically, the procedure sets out to amplify small quantity of microbes into several million copies, followed by the detection of nucleic acid on a specialized medium. 25/10/2022 17
  • 18. • Haematology: This laboratory is involved in the performance of relevant tests (on blood) in the diagnosis of blood diseases, (e.g. Anaemia, Haemoglobinopathies, Leukaemia etc) and blood transfusion services e.g. blood group, blood cross matching. 25/10/2022 18
  • 19. • Chemical Pathology Laboratory (Clinical Chemistry or Clinical Biochemistry Laboratory): This division of laboratory is concerned with the performance of quantitative and qualitative tests on clinical specimens to investigate the state of various body chemistries. • Such clinical specimens include body fluids (e.g. whole blood, plasma, serum, urine, sweat, cerebrospinal fluid) and occasionally faeces, tissue, hair e.t.c. 25/10/2022 19
  • 20. • Histopathology Laboratory: This is the laboratory where tissues (or cells) are processed for microscopic examination in order to investigate or study disease manifestations on the tissue (or cells), structure, for diagnostic purposes e.g. Cancer diagnosis. • In the laboratory, tissue samples are processed onto glass slides from which effects of diseases on the histological architecture of tissues can be microscopically examined and hence diagnostic inferences are made e.g. Cancer diagnosis. 25/10/2022 20