The document describes histopathology results showing fluorescent microspheres adhering to atherosclerotic plaques in an Apo E mouse model after 24 hours. One macrophage was seen containing 10 fluorescent microspheres adhering to a fibrous plaque. An aggregation of 4 labeled macrophages was attached to an advanced plaque. Numerous macrophages were adhering to an isolated fatty streak comprised mainly of foam cells.

1. Histopathology Results of
biodistribution of SPIO in
Apo E Mouse
After 24 hours

3. Adhering Circumferentially to Atherosclerotic Plaque. One
Macrophage Containing 10 Fluorescent microspheres (C and D)
Is Seen Adhering to a Fibrous Plaque(with hematoxylin with the
use of a xylene-free method)

4. One macrophage containing 10
fluorescent microspheres (c and
d) is seen adhering to a fibrous
plaque. One aggregation of 4
labeled macrophages (e and f)
is seen attached to an advanced
plaque. Numerous
macrophages (g and h) are
seen adhering to this isolated
fatty streak, which is mostly
comprised of foam cells. A
collection of foam cells overlying
a fibrous plaque attracts labeled
macrophages (i and j). A few
microspheres are seen within
the area of foam cells,
suggesting transmigration of the
macrophage across the
endothelial cell layer.

5. Transvers section Descending
Aorta
Movat’s stainingPearl’s

6. Transvers section Descending
Aorta(diaphragm level)
H&EPearl’s

7. Transvers section Descending
Aorta (diaphragm level)
CD60Pearl’s

8. Transvers section Descending
Aorta (diaphragm level)
CD6o H&EPearl’s

9. Transverse Section of Aorta
Valve Region
Movat’sPearl’s

10. Transverse Section of Aorta
Valve Region
H&EPearl’s

11. Transverse Section of Aorta
Valve Region
CD60Pearl’s

12. Transverse Section of Aorta
Valve Region
CD60 H&EPearl’s

13. Liver