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RICE 
Oryza sativa L.
Rice (Oryza sativa L.) 
■the most important staple food for a large 
part of the world's human population: 
 East Asia 
 South Asia 
 Southeast Asia 
 Middle East 
 Latin America, and 
 West Indies 
■the grain with the second highest worldwide 
production, after maize.
Expansion of rice cultivation 
Asian Rice 
Oryza sativa Wild Rice
Rice-Producing Countries
Rice Production and Consumption
Rice-Producing Countries 
Together, China and India accounted for over half of the 
world’s rice supply in 2006. 
1. China: 182 million tons (28.8%) 
2. India: 136.5 million tons (21.6%) 
3. Indonesia: 54.4 million tons (8.6%) 
4. Bangladesh: 43.7 million tons (6.9%) 
5. Vietnam: 35.8 million tons (5.7%) 
6. Thailand: 29.3 million tons (4.6%) 
7. Myanamar: 25.2 million tons (4%) 
8. Philippines: 15.3 million tons (2.4%) 
9. Brazil: 11.5 million tons (1.8%) 
10. Japan: 10.7 million tons (1.7%). 
http://internationaltradecommodities.suite101.com/article.cfm/top_rice_producing_countries#ixzz0snCNSXzS
Taxonomy 
Kingdom: Plantae (Plants) 
Subkingdom: Tracheobionta (Vascular plants) 
Superdivision: Spermatophyta (Seed plants) 
Division: Magnoliophyta (Flowering plants) 
Class: Liliopsida (Monocotyledons) 
Subclass: Commelinidae 
Order: Cyperales 
Family: Poaceae (Grass family) 
Genus: Oryza L. (rice) 
Species: Oryza sativa L. (rice)
History 
■Rice is the world's largest food crop, 
providing the caloric needs of millions 
of people daily 
■There are two distinct types of 
domesticated rice 
 Oryza sativa, or Asian rice and 
 Oryza glaberrima, African rice.
History 
■The genus Oryza contains 21 wild 
relatives 
■The genus is divided into four species: 
 O. sativa, 
 O. officialis, 
 O. ridelyi 
 O. granulata species complexes 
■All members of the Oryza genus have n 
= 12 chromosomes
History 
■O. sativa complex contains 2 
domesticated species: 
 O. sativa 
 O. glaberrima,, and 5 or 6 wild species: 
 O. rujipogon, 
 O. nivara (also considered to be an ecotype 
of O. rujipogon), 
 O. barthii, 
 O. longistaminata, 
 O. meridionalis and 
 O. glumaepatula, all of which are diploids.
History 
 Oryza sativa: Asia 
 O. glaberrima: West Africa. 
 Oryza rujipogon: Asia and Oceania. 
 O. barthii and O. longistaminata: African 
 O. barthii:endemic in West Africa 
 O. longistaminata: throughout Africa. 
 Oryza meridionalis: native to Australia 
 O. glumaepatula: endemic in Central and 
South America.
History 
■Many phenotypic differences are 
obvious between O. sativa and its wild 
relatives 
■Wild rices typically display long awns 
and severe shattering for seed 
dispersal, whereas the domesticated 
type have short awns if any and 
reduced shattering to maximize the 
number of seeds that can be harvested.
Botany
Germinating seed 
The coleorhiza protrudes first Radicle or primary root breaks 
through the covering 
Coleoptile emerging as a 
tapered cylinder
Seedling 
Mesocotyl pushing the coleoptile 
above the soil surface 
First and second seedling leaf
Tiller 
Types of roots Secondary adventitious roots
Culm 
Young and mature culm
Tillers
Leaf 
Leaves 
alternate on 
the culm in 
opposite 
directions
Leaf 
Sheath pulvinus Leaf collar Ligule and auricle 
Parallel veins on upper surface Leaf midrib 
Rice and grassy weed comparison 
Different varieties with varying blade characteristics
Panicle and Spikelet 
Rice panicle
Panicle and Spikelet
Floret
Flower
Rice grain 
Rice grains Rice hulls
WHAT IS ORGANIC RICE?
Organic Rice 
■Free from chemical 
■fat free and high carbohydrate content 
■red and brown rice have higher fiber 
value than white rice 
■has low sugar content 
■When you eat 45 grams of organic rice, 
you only consume 160 calories of 
carbohydrates.
Organic Rice production 
■avoid the use of synthetic fertilizers, 
pesticides, and growth regulators 
■Instead they rely on crop rotations 
■biological pest control 
■maintain soil health 
■supply plant nutrients 
■minimize insects, weeds, and other 
pests.
Weed Suppression 
■Weed control and soil fertility are the 
principal challenges 
■primary weed-control practices include 
 crop rotations 
 land leveling 
 seedbed preparation 
■water management 
■rotary hoeing.
Soil Fertility 
■Maintaining soil fertility in organic 
cropping typically involves some 
combination of crop rotation with 
deep-rooted legume crops or green 
manure/cover crops 
■applying 
 animal manures 
 composts 
 other approved organic amendments.
Soil Fertility 
■Nitrogen is usually provided through 
the growing of legume cover crops 
■Bone meal is a good source of cheap 
phosphorus (content of around 12%) 
■Rice straw and manure are good 
sources of potassium.
Organic manure 
■Organic manures obtained either from 
cattle or plants 
■Organic manure's – maintains better 
structure of soil 
■Green leaf manure 
 mainly neem, Pongamia pinnata and 
Glyricidia 
■Green manure 
 daincha, manila agathi, sunhemp.
Insects and diseases management 
■Rice is grown in flooded fields, insect 
pests are usually a minor problem 
■Fall armyworm and chinchbug are 
easily controlled by a flush of water 
■Timely planting, variety selection, and 
cultural practices to suppress 
 weeds control 
 stinkbugs and 
 water weevils.
Biofertilizers for rice cultivation 
■Bio-fertilizer are eco friendly and are 
environmentally safe 
■The biofertilizers used for rice crop 
 Azospirillum 
 Phosphobacteria 
 Blue green algae Azolla 
 Mycorhiza.
Application of biofertilizers 
■Azospirillum: is a symbiotic bacteria and 
it is an important biofertilizers used in 
rice 
■Azospirillum treatment is 
recommended for seed, seedlings and 
in main field 
■Seed treatment: 600 g/ha of 
Azospirillum culture are to be mixed 
with water where the seeds are soaked 
one night before sowing in the nursery 
bed.
Application of biofertilizers 
■Main field: 2000 g/ha of Azospirillum 
with 25 kg farmyard mamure and 25 kg 
of soil are mixed uniformly and 
broadcasted in the main field before 
transplanting 
■Seedling treatment: A slurry can be 
prepared by mixing Azospirillum at 
1000 g/ha in 40 litres of water and root 
portion of transplanted rice seedlings 
are dipped in bacterial suspension for 
15-30 minutes.
Uses 
■Azospirillum bacteria thrives in root zones of 
rice and is capable of fixing more atmospheric 
nitrogen which is absorbed by the plants 
■Azospirillum also solubilizes phosphorus and 
silicon to some extent required by rice 
■It renders plant drought tolerant when 
irrigation or rainfall is delayed 
■By adopting Azospirillum application 30 per 
cent of the inorganic nitrogen usage can be 
reduced.
Blue Green Algae (BGA) 
 Blue Green Algae (BGA) is another 
type of biofertilizer used as an alga 
form. Important species are 
Cyanobacteria, Anabaena, Nostoc 
and Tolypothrix 
 Blue green algal occurs naturally 
and comes up well under moist 
conditions. This can also be 
artificially cultured.
Uses 
■The nitrogen fixed by BGA is about 15 
kg/ha over a season 
■It oxygenates the water impounded in 
the field 
■It excretes organic acids that renders 
phosphorus solubilisation 
■The algal mat in paddy fields also 
protects loss of moisture from the soil.
Azolla 
 Azolla is a water fern having an algal 
symbiont Anabaena azolla fixing 
atmospheric nitrogen 
 Azolla can be multiplied by 
constructing nurseries with 10 cm 
deep standing water and adding 
superhphosphate at 8 kg P2O5/ha in 
small plots 
 It can be applied as green manure 
prior to rice planting
Uses 
■Azolla excretes organic nitrogen in water 
during its growth and also immediately upon 
trampling 
■Fern fronds are soft and rapidly decomposed. 
■It absorbs traces of potassium from irrigation 
water 
■Azolla provides nitrogen, potassium organic 
carbon etc. 
■It prevents weed growth in rice field water.
Phosphobacteria 
■This type of biofertilizers solubilise 
phosphates in the soil and render them 
in available form for crop plants 
■This is applied as the same dose in 
same manner as Azospirillum.
Uses 
■25 to 50 of the recommended 
Phosphorus can be reduced depending 
upon the native phosphorus content of 
the soil.
Mycorrhiza 
■It occurs naturally in low land and 
upland rice. It mobilizes the 
phosphorus required by rice 
■Since, biofertilizers are ecofriendly 
inputs and are safer to the 
environment, farmers can adopt them 
and get benefited.
WHAT IS HYBRID RICE?
Hybrid Rice 
■Hybrids are produced by crossing two 
inbred - genetically fixed - varieties of a 
particular crop 
■Hybrids are special because they 
express what is called "heterosis" or 
hybrid vigour 
■The idea is that if you cross two parents 
which are genetically distant from each 
other, the offspring will be "superior", 
particularly in terms of yield.
Heterosis (hybrid vigor) 
■application to increase: 
 productivity (yield/unit/time, 15-20% of 
yield advantage), and 
 economic returns 
■Heterosis 
 A universal phenomenon that F1 
generation shows superiority to both 
parents in agronomic traits or yield 
 has been exploited commercially in many 
agricultural crops.
Normal Rice Spikelet 
(self pollinated crop) 
Sterile Rice Spikelet 
(Male Sterility) 
Hybrid Seed Production 
(Male Sterile x Normal Rice) 
How Hybrid Rice?
Male Sterility Systems in Rice 
■Male sterility: a condition in which the 
pollen grain is unviable or cannot 
germinate and fertilize normally to set 
seeds 
■Male Sterility Systems (genetic and 
non-genetic): 
 Cytoplasmic genetic male sterility (CMS) 
 Male sterility is controlled by the 
interaction of a genetic factor (S) present 
in the cytoplasm and nuclear gene (s).
Male Sterility Systems in Rice 
 Environment-sensitive genic male sterility 
(EGMS) 
 Male sterility system is controlled by 
nuclear gene expression, which is 
influenced by environmental factors such 
as 
 temperature (TGMS) 
 daylength (PGMS), or 
 both (TPGMS). 
 Chemically induced male sterility 
 Male sterility is induced by some chemicals 
(gametocides)
Release of IRRI Hybrids in Different Countries (1994-2005) 
IRRI Hybrid Released as Country Year released 
IR64610H MGR-1 India 1994 
IR64611H KRH-1 India 1994 
IR64616H Magat Philippines 1994 
IR65489H DRRH-1 India 1996 
IR68284H Mestizo 1 Philippines 1997 
IR69690H Sahyadri India 1998 
IR69690H HYT-57 Vietnam 1999 
IR69690H BRRI Dhan Hybrid 1 Bangladesh 2001 
IR69690H Rokan Indonesia 2002 
IR75207H Mestizo 2 Philippines 2002 
IR75217H Mestizo 3 Philippines 2002 
IR78386H Mestizo 7 Philippines 2005
“Super high-yielding” hybrid rice breeding in China 
Progress of "Super High-Yielding" Hybrid Rice Program in China 
Goal (single-season) Progress (2006) 
Stage Yield (t/ha) Years Breeding Commercialization 
Start 8.25 1997 
Phase I 10.5 1996 - 2000 Finished Large area extension 
Phase II 12.0 2000 - 2005 Finished Started 
Phase III 13.5 2005 - 2010 Started
Hybrid Rice
Hybrid Rice Seed Production 
In Asia In United Sates
WHAT IS GOLDEN RICE?
Chronic Undernutrition among Children 
Percentage of 
stunted children
Estimated Annual Number of Child Deaths 
Precipitated by Vitamin A Deficiency (Asia) 
600 8,000 
30,500 
14,000 
2,000 300 
13,000 6,900 
56,000 
4,500 1,400 2,000 
28,000 
330,000 
350000 
300000 
250000 
200000 
150000 
100000 
50000 
0 
Bhutan 
Cambodia 
Bangladesh 
China 
India 
Indonesia 
Myanmar 
Lao PDR 
Mongolia 
Philippines 
Nepal 
Pakistan 
Thailand 
Viet Nam 
Estimated Annual Number
VAD in China – current situation 
■The deaths of over 20,000 children each 
year from increased susceptibility to 
infection. 
 Cause: vitamin A deficiency 
■Approximately 12% of China's children 
growing up with lowered immunity, leading 
to frequent ill health and poor growth. 
 Cause: vitamin A deficiency (the prevalence 
of vitamin A deficiency in children under 6 is 
estimated at 12%). 
Vitamin & Mineral Deficiency: A damage assessment report for China. 
Micronutrient Initiative and UNICEF 2004
Trends in the Prevalence of Vitamin A 
Deficiency 
(by Age, and by Physiological State: 
1993 & 1998) 
35.3 
Philippine Nutrition: Facts and Figures 
(National Nutrition Survey, 1998) 
16.4 16.4 
38.0 
22.2 
16.5 
50.0 
40.0 
30.0 
20.0 
10.0 
0.0 
Children Pregnant Women Lactating Women 
Percent Prevalence 
1993 1998
What Is Golden Rice? 
■is created through recombinant 
technology, genetically engineering the 
rice to produce β-carotene 
■by introducing 4 extra genes for the 
respective enzymes into the 
endosperm 
■The enzymes are required to complete 
the biosynthetic pathway in order for 
the rice to synthesize and accumulate 
β-carotene or pro-vitamin A.
What Is Golden Rice? 
■The genes are isolated from Narcissus 
pseudonarcissus (daffodil) 
■Theses genes code for the enzymes: 
 phytoene synthase (psy) 
 phytoene desaturase and 
 lycopene β-cyclase. 
■The bacterial enzyme carotene 
desaturase is utilized to simplify the 
transformation process.
Basic Carotenoid Biosynthetic Pathway 
IPP DMAPP 
GGPP (C20) 
Phytoene (C40) 
desaturation 
Lycopene (C40) 
cyclization 
Carotenes 
Phytoene synthase 
Plant source 
Phytoene desaturase 
Bacterial source 
a - carotene b - carotene 
b-cryptoxanthin 
zeaxanthin 
canthaxanthin 
astaxanthin 
Xanthophylls 
a-cryptoxanthin 
lutein 
(Lycopene cyclase) 
Bacterial source
ANY QUESTIONS?
THANK YOU

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4 rice

  • 2. Rice (Oryza sativa L.) ■the most important staple food for a large part of the world's human population:  East Asia  South Asia  Southeast Asia  Middle East  Latin America, and  West Indies ■the grain with the second highest worldwide production, after maize.
  • 3. Expansion of rice cultivation Asian Rice Oryza sativa Wild Rice
  • 5. Rice Production and Consumption
  • 6. Rice-Producing Countries Together, China and India accounted for over half of the world’s rice supply in 2006. 1. China: 182 million tons (28.8%) 2. India: 136.5 million tons (21.6%) 3. Indonesia: 54.4 million tons (8.6%) 4. Bangladesh: 43.7 million tons (6.9%) 5. Vietnam: 35.8 million tons (5.7%) 6. Thailand: 29.3 million tons (4.6%) 7. Myanamar: 25.2 million tons (4%) 8. Philippines: 15.3 million tons (2.4%) 9. Brazil: 11.5 million tons (1.8%) 10. Japan: 10.7 million tons (1.7%). http://internationaltradecommodities.suite101.com/article.cfm/top_rice_producing_countries#ixzz0snCNSXzS
  • 7. Taxonomy Kingdom: Plantae (Plants) Subkingdom: Tracheobionta (Vascular plants) Superdivision: Spermatophyta (Seed plants) Division: Magnoliophyta (Flowering plants) Class: Liliopsida (Monocotyledons) Subclass: Commelinidae Order: Cyperales Family: Poaceae (Grass family) Genus: Oryza L. (rice) Species: Oryza sativa L. (rice)
  • 8. History ■Rice is the world's largest food crop, providing the caloric needs of millions of people daily ■There are two distinct types of domesticated rice  Oryza sativa, or Asian rice and  Oryza glaberrima, African rice.
  • 9. History ■The genus Oryza contains 21 wild relatives ■The genus is divided into four species:  O. sativa,  O. officialis,  O. ridelyi  O. granulata species complexes ■All members of the Oryza genus have n = 12 chromosomes
  • 10. History ■O. sativa complex contains 2 domesticated species:  O. sativa  O. glaberrima,, and 5 or 6 wild species:  O. rujipogon,  O. nivara (also considered to be an ecotype of O. rujipogon),  O. barthii,  O. longistaminata,  O. meridionalis and  O. glumaepatula, all of which are diploids.
  • 11. History  Oryza sativa: Asia  O. glaberrima: West Africa.  Oryza rujipogon: Asia and Oceania.  O. barthii and O. longistaminata: African  O. barthii:endemic in West Africa  O. longistaminata: throughout Africa.  Oryza meridionalis: native to Australia  O. glumaepatula: endemic in Central and South America.
  • 12. History ■Many phenotypic differences are obvious between O. sativa and its wild relatives ■Wild rices typically display long awns and severe shattering for seed dispersal, whereas the domesticated type have short awns if any and reduced shattering to maximize the number of seeds that can be harvested.
  • 14. Germinating seed The coleorhiza protrudes first Radicle or primary root breaks through the covering Coleoptile emerging as a tapered cylinder
  • 15. Seedling Mesocotyl pushing the coleoptile above the soil surface First and second seedling leaf
  • 16. Tiller Types of roots Secondary adventitious roots
  • 17. Culm Young and mature culm
  • 19. Leaf Leaves alternate on the culm in opposite directions
  • 20. Leaf Sheath pulvinus Leaf collar Ligule and auricle Parallel veins on upper surface Leaf midrib Rice and grassy weed comparison Different varieties with varying blade characteristics
  • 21. Panicle and Spikelet Rice panicle
  • 25. Rice grain Rice grains Rice hulls
  • 27. Organic Rice ■Free from chemical ■fat free and high carbohydrate content ■red and brown rice have higher fiber value than white rice ■has low sugar content ■When you eat 45 grams of organic rice, you only consume 160 calories of carbohydrates.
  • 28. Organic Rice production ■avoid the use of synthetic fertilizers, pesticides, and growth regulators ■Instead they rely on crop rotations ■biological pest control ■maintain soil health ■supply plant nutrients ■minimize insects, weeds, and other pests.
  • 29. Weed Suppression ■Weed control and soil fertility are the principal challenges ■primary weed-control practices include  crop rotations  land leveling  seedbed preparation ■water management ■rotary hoeing.
  • 30. Soil Fertility ■Maintaining soil fertility in organic cropping typically involves some combination of crop rotation with deep-rooted legume crops or green manure/cover crops ■applying  animal manures  composts  other approved organic amendments.
  • 31. Soil Fertility ■Nitrogen is usually provided through the growing of legume cover crops ■Bone meal is a good source of cheap phosphorus (content of around 12%) ■Rice straw and manure are good sources of potassium.
  • 32. Organic manure ■Organic manures obtained either from cattle or plants ■Organic manure's – maintains better structure of soil ■Green leaf manure  mainly neem, Pongamia pinnata and Glyricidia ■Green manure  daincha, manila agathi, sunhemp.
  • 33. Insects and diseases management ■Rice is grown in flooded fields, insect pests are usually a minor problem ■Fall armyworm and chinchbug are easily controlled by a flush of water ■Timely planting, variety selection, and cultural practices to suppress  weeds control  stinkbugs and  water weevils.
  • 34. Biofertilizers for rice cultivation ■Bio-fertilizer are eco friendly and are environmentally safe ■The biofertilizers used for rice crop  Azospirillum  Phosphobacteria  Blue green algae Azolla  Mycorhiza.
  • 35. Application of biofertilizers ■Azospirillum: is a symbiotic bacteria and it is an important biofertilizers used in rice ■Azospirillum treatment is recommended for seed, seedlings and in main field ■Seed treatment: 600 g/ha of Azospirillum culture are to be mixed with water where the seeds are soaked one night before sowing in the nursery bed.
  • 36. Application of biofertilizers ■Main field: 2000 g/ha of Azospirillum with 25 kg farmyard mamure and 25 kg of soil are mixed uniformly and broadcasted in the main field before transplanting ■Seedling treatment: A slurry can be prepared by mixing Azospirillum at 1000 g/ha in 40 litres of water and root portion of transplanted rice seedlings are dipped in bacterial suspension for 15-30 minutes.
  • 37. Uses ■Azospirillum bacteria thrives in root zones of rice and is capable of fixing more atmospheric nitrogen which is absorbed by the plants ■Azospirillum also solubilizes phosphorus and silicon to some extent required by rice ■It renders plant drought tolerant when irrigation or rainfall is delayed ■By adopting Azospirillum application 30 per cent of the inorganic nitrogen usage can be reduced.
  • 38. Blue Green Algae (BGA)  Blue Green Algae (BGA) is another type of biofertilizer used as an alga form. Important species are Cyanobacteria, Anabaena, Nostoc and Tolypothrix  Blue green algal occurs naturally and comes up well under moist conditions. This can also be artificially cultured.
  • 39. Uses ■The nitrogen fixed by BGA is about 15 kg/ha over a season ■It oxygenates the water impounded in the field ■It excretes organic acids that renders phosphorus solubilisation ■The algal mat in paddy fields also protects loss of moisture from the soil.
  • 40. Azolla  Azolla is a water fern having an algal symbiont Anabaena azolla fixing atmospheric nitrogen  Azolla can be multiplied by constructing nurseries with 10 cm deep standing water and adding superhphosphate at 8 kg P2O5/ha in small plots  It can be applied as green manure prior to rice planting
  • 41. Uses ■Azolla excretes organic nitrogen in water during its growth and also immediately upon trampling ■Fern fronds are soft and rapidly decomposed. ■It absorbs traces of potassium from irrigation water ■Azolla provides nitrogen, potassium organic carbon etc. ■It prevents weed growth in rice field water.
  • 42. Phosphobacteria ■This type of biofertilizers solubilise phosphates in the soil and render them in available form for crop plants ■This is applied as the same dose in same manner as Azospirillum.
  • 43. Uses ■25 to 50 of the recommended Phosphorus can be reduced depending upon the native phosphorus content of the soil.
  • 44. Mycorrhiza ■It occurs naturally in low land and upland rice. It mobilizes the phosphorus required by rice ■Since, biofertilizers are ecofriendly inputs and are safer to the environment, farmers can adopt them and get benefited.
  • 45. WHAT IS HYBRID RICE?
  • 46. Hybrid Rice ■Hybrids are produced by crossing two inbred - genetically fixed - varieties of a particular crop ■Hybrids are special because they express what is called "heterosis" or hybrid vigour ■The idea is that if you cross two parents which are genetically distant from each other, the offspring will be "superior", particularly in terms of yield.
  • 47. Heterosis (hybrid vigor) ■application to increase:  productivity (yield/unit/time, 15-20% of yield advantage), and  economic returns ■Heterosis  A universal phenomenon that F1 generation shows superiority to both parents in agronomic traits or yield  has been exploited commercially in many agricultural crops.
  • 48. Normal Rice Spikelet (self pollinated crop) Sterile Rice Spikelet (Male Sterility) Hybrid Seed Production (Male Sterile x Normal Rice) How Hybrid Rice?
  • 49. Male Sterility Systems in Rice ■Male sterility: a condition in which the pollen grain is unviable or cannot germinate and fertilize normally to set seeds ■Male Sterility Systems (genetic and non-genetic):  Cytoplasmic genetic male sterility (CMS)  Male sterility is controlled by the interaction of a genetic factor (S) present in the cytoplasm and nuclear gene (s).
  • 50. Male Sterility Systems in Rice  Environment-sensitive genic male sterility (EGMS)  Male sterility system is controlled by nuclear gene expression, which is influenced by environmental factors such as  temperature (TGMS)  daylength (PGMS), or  both (TPGMS).  Chemically induced male sterility  Male sterility is induced by some chemicals (gametocides)
  • 51. Release of IRRI Hybrids in Different Countries (1994-2005) IRRI Hybrid Released as Country Year released IR64610H MGR-1 India 1994 IR64611H KRH-1 India 1994 IR64616H Magat Philippines 1994 IR65489H DRRH-1 India 1996 IR68284H Mestizo 1 Philippines 1997 IR69690H Sahyadri India 1998 IR69690H HYT-57 Vietnam 1999 IR69690H BRRI Dhan Hybrid 1 Bangladesh 2001 IR69690H Rokan Indonesia 2002 IR75207H Mestizo 2 Philippines 2002 IR75217H Mestizo 3 Philippines 2002 IR78386H Mestizo 7 Philippines 2005
  • 52. “Super high-yielding” hybrid rice breeding in China Progress of "Super High-Yielding" Hybrid Rice Program in China Goal (single-season) Progress (2006) Stage Yield (t/ha) Years Breeding Commercialization Start 8.25 1997 Phase I 10.5 1996 - 2000 Finished Large area extension Phase II 12.0 2000 - 2005 Finished Started Phase III 13.5 2005 - 2010 Started
  • 54. Hybrid Rice Seed Production In Asia In United Sates
  • 55. WHAT IS GOLDEN RICE?
  • 56. Chronic Undernutrition among Children Percentage of stunted children
  • 57. Estimated Annual Number of Child Deaths Precipitated by Vitamin A Deficiency (Asia) 600 8,000 30,500 14,000 2,000 300 13,000 6,900 56,000 4,500 1,400 2,000 28,000 330,000 350000 300000 250000 200000 150000 100000 50000 0 Bhutan Cambodia Bangladesh China India Indonesia Myanmar Lao PDR Mongolia Philippines Nepal Pakistan Thailand Viet Nam Estimated Annual Number
  • 58. VAD in China – current situation ■The deaths of over 20,000 children each year from increased susceptibility to infection.  Cause: vitamin A deficiency ■Approximately 12% of China's children growing up with lowered immunity, leading to frequent ill health and poor growth.  Cause: vitamin A deficiency (the prevalence of vitamin A deficiency in children under 6 is estimated at 12%). Vitamin & Mineral Deficiency: A damage assessment report for China. Micronutrient Initiative and UNICEF 2004
  • 59. Trends in the Prevalence of Vitamin A Deficiency (by Age, and by Physiological State: 1993 & 1998) 35.3 Philippine Nutrition: Facts and Figures (National Nutrition Survey, 1998) 16.4 16.4 38.0 22.2 16.5 50.0 40.0 30.0 20.0 10.0 0.0 Children Pregnant Women Lactating Women Percent Prevalence 1993 1998
  • 60. What Is Golden Rice? ■is created through recombinant technology, genetically engineering the rice to produce β-carotene ■by introducing 4 extra genes for the respective enzymes into the endosperm ■The enzymes are required to complete the biosynthetic pathway in order for the rice to synthesize and accumulate β-carotene or pro-vitamin A.
  • 61. What Is Golden Rice? ■The genes are isolated from Narcissus pseudonarcissus (daffodil) ■Theses genes code for the enzymes:  phytoene synthase (psy)  phytoene desaturase and  lycopene β-cyclase. ■The bacterial enzyme carotene desaturase is utilized to simplify the transformation process.
  • 62. Basic Carotenoid Biosynthetic Pathway IPP DMAPP GGPP (C20) Phytoene (C40) desaturation Lycopene (C40) cyclization Carotenes Phytoene synthase Plant source Phytoene desaturase Bacterial source a - carotene b - carotene b-cryptoxanthin zeaxanthin canthaxanthin astaxanthin Xanthophylls a-cryptoxanthin lutein (Lycopene cyclase) Bacterial source
  • 63.
  • 64.