“Perio-Spardha” Program on Autologous Platelet Concentrates-Two lectures on “Tweaking the Centrifuge- An important protocol in the generation of PRF” and “Applications and Limitations of PRF in Periodontics and Implantology”. Organized by Bangalore Academy of Periodontology (BAP) and Indian Society of Periodontology at Oxford Dental College Hospital and Research Centre, Bangalore, India on 14/02/2017.

4. CENTRIFUGE

6. L-PRF 2700 RPM 12 MINUTES ~400g 33°

8. 1,65,000 INR
24,000 INR

9. Remi C854
Universal/Mini/Low cost
6100 INR

10. L-PRF 2700 RPM 12 MINUTES ~400g 33°
CORRECT RPM TIME G Force ANGULATION

11. G Force
ANGULATION
CORRECT RPM
TIME

12. Angulation ~33°
A°α RPM
ROULEAU
FIBRINOGEN
SEDIMENTATION

18. Increased tube angulation > 33° leads to
1. Increased tube vibration and parasitic
acceleration
2. Possible cell death in L-PRF
3. Slow release of mediators

20. G force & RPM

22. G force= 1.12 x r x (RPM/1000)2

23. G force= 1.12 x r x (RPM/1000)2

24. G force= 1.12 x r x (RPM/1000)2
1.12 x 125 x (6000/1000)2
1.12 x 130 x (3500/1000)2

25. G force= 1.12 x r x (RPM/1000)2
1.12 x 125 x (2700/1000)2
1.12 x 130 x (2700/1000)2

26. TWEAKING the CENTRIFUGE

27. PRF protocol is an OPEN ACCESS SYSTEM*

28. PRF protocol is an OPEN ACCESS SYSTEM*
~750g

29. One size doesn’t fit all*

30. . Check for the tube angulation
And there’s nothing you can do about it……

31. G force= 1.12 x R x (RPM/1000)2
Length of the tube Central axis to mouth
distance
G force= 1.12 x 116 x (RPM/1000)2

32. G force= 1.12 x 116 x (RPM/1000)2

34. L-PRF 2700 RPM 12 MINUTES ~400g

35. L-PRF 2700 RPM ~400g

36. WHAT ABOUT THE TIME?

37. Sedimentation Rate: is a measure of the
cells sedimenting in a tube over a given
period of time.
Time for a clinical protocol and for a
therapeutic endpoint is CONSTANT.

38. STOKES EQUATION
Sedimentation rate increases as the
gravitational force increases
Sedimentation TIME decreases as the
gravitational force increases

39. L-PRF 2700 RPM 12 MINUTES ~400g
DONT CHANGE THE TIME

41. RESEARCH IN MOTION?
G force at 2700
rpm
Adjusted RPM for
400g force
DUOS 898 Not recommended
Remi 8C 1020 1690
Remi C845 1061 1650

42. COMPARE OPTICAL DENSITY (OD) AND
FIBRINOGEN CONTENT OF PRF BY STANDARD
PROTOCOL (2700 RPM/12MINS) VS REVISED
PROTOCOL (1650 RPM/12MINS)

44. REVISED PROTOCOL
Mean
SD
Fvalue
Pvalue
OPTICAL
DENSITY
C845 1.07 .79
2.596 .086
REMI8C 2.04 2.72
DUOS
.73 .23
FIBRINOGEN
C845 34.54 25.57
1.384 .262REMI8C 29.85 23.91
DUOS 22.29 4.04

45. Mean SD t value P value
OD
C 845
Standard
Revised
OD Remi8C
Standard
Revised
1.07113
1.12013
2.04940
1.09213
.795078
1.813317
2.720886
.689743
096
1.321
.0924
.197
FIBRINOGEN
C 845
Standard
Revised
REMI 8C
Standard
Revised
34.54198
20.48585
29.8598
35.4160
25.577454
17.577644
23.91254
22.36728
1.754
0.657
.090
.516

46. 1. LABORATORY CENTRIFUGES RESULTED IN
DENSER CLOT AND HIGHER FIBRINOGEN
CONTENT OVER FIXED ANGLE CENTRIFUGE
(DUOS)
2. C845 RESULTED IN HIGHER FIBRINOGEN
CONTENT AND LESS DENSER PRF OVER REMI
8C
3. HOWEVER, REVISED PROTOCOL RESULTED IN
REMI 8C PRODUCING PRF WITH HIGHEST
FIBRINOGEN CONTENT.

48. PRF BOX
97% Platelets
>50% Leucocytes
Densest
Platelet + Fibrin

49. First 2mm
Highest P/L Density

50. Serum exudation

51. PRF BOX
PRF Container Box
PRF Expression Box
PRF Containment Box
Gravitationally or Mechanically press the
PRF into desired shape
Collect the serum fluid

52. IS PRF BOX A “PLAIN STEEL BOX”?

54. PRF BOX results in
Consistent membrane
thickness
Gravitational
compression
Easier exudate
collection

55. BLOOD
THE BIGGEST
LIMITING FACTOR
Draw good volumes
Draw blood quickly
Process it faster

56. The plastic tubes contain a
silicate coating that initiates the
clumping of the RBC’s
(Rouleau formation)
Glass tubes have natural silica
so that a coating is not
necessary for separation.

57. DRAWING ENOUGH BLOOD QUICKLY
Plastic is a Hydrophobic surface
Inefficient activation of clotting cascade
Gelatinous clots instead of fibrous clots

58. The L-PRF protocol
requires the use of a
RED top vacutainer.
This color indicates that
there is no anti-
coagulant present.

59. Serum tube; No additive,
Non-siliconized; Sterile
Used for AFG and iPRF

60. WHAT VOLUME IS ADEQUATE VOLUME?

61. WHAT VOLUME IS ADEQUATE VOLUME?