1. Use the appropriate fixative for your sample 2. Choose the right detergent to stain your target. 3. Use appropriate controls. 4. optimize antibody dilution and cell density. 5. Use blocking serum from a different organism than the primary antibody. 6. Primary and secondary antibody optimization. 7. Multiple staining steps (optional). 8. Employ counterstains to help identify cellular landmarks. 9. Get a proper sample mounting?

1. 9 Tips to Optimize Antibody Your If
Experiments
antibody humanization
single domain antibody
antibody discovery

2. 1. Use the appropriate fixative for your sample

3. 2. Choose the right detergent to stain your
target.

4. 3. Use appropriate controls.

5. 4. optimize antibody dilution and cell density.

6. 5. Use blocking serum from a different
organism than the primary antibody.

7. 6. Primary and secondary antibody
optimization.

8. 7. Multiple staining steps (optional).

9. 8. Employ counterstains to help identify
cellular landmarks.

10. 9. Get a proper sample mounting?