Dr. Bill Andrews on telomeres, their biology, and importance.

1. Bill Andrews, Ph.D.
 Discovered Human Telomerase
 2nd Place United States Inventor of the Year
 Telomere Biologist for 19 Years
 31 Years in Biotech Research
 43 U.S. Issued Patents on Telomerase
 Founder, President & CEO Sierra Sciences
 Popular Science, The Today Show, etc.

2. Short
Telomere
Disease

3. Bad Things
Happen When
Telomeres
Get Short!
Telomere

4. Telomere

11. Congratulations
Elizabeth Blackburn, Carol Greider, and Jack Szostak
2009 Nobel Prize in Medicine!

14. Some People are Born with Short
Telomeres

15. Progeria
Mech Ageing Dev. 2009 Jun;130(6):377-83. Epub 2009 Mar 20.
Short Telomere
Disease

16. Progeria
Mech Ageing Dev. 2009 Jun;130(6):377-83. Epub 2009 Mar 20.
Short Telomere
Disease
Life Expectancy - 20 Years

17. Progeria
Mech Ageing Dev. 2009 Jun;130(6):377-83. Epub 2009 Mar 20.
Short Telomere
Disease
Life Expectancy - 20 Years
Preventing Telomere Shortening
Could Cure These Kids!

18. Bad Things
Happen When
Telomeres
Get Short!

19. Normal Health & Aging

20. Short Telomere Diseases
• Cardiovascular
• Cancer
• COPD
• Degenerative Disc Disease
• Alzheimer’s
• Osteoarthritis
• Rheumatoid Arthritis
• Osteoporosis
• General Immunity
• Skin Aging
• Macular Degeneration
• Liver Cirrhosis
• Muscular Dystrophy
• Cell & Tissue Transplants
• AIDS
• Progeria
• Dyskeratosis Congenita
• Idiopathic Pulmonary Fibrosis
• Cri du Chat syndrome
• Down’s Syndrome
• Fanconi’s Anemia
• Tuberous Sclerosis
• Werner’s Syndrome
• And, Aging Itself???????

21. Bad Things
Happen When
Telomeres
Get Short!

22. Dr. David
Woynarowski

23. 1971: Nixon declares War on Cancer

24. War on
Short Telomere
Disease!

25. September 2-8
is
Telomere
Research
Week

26. What Can Be
Done About
Telomere
Shortening?

27. Managing Telomere Length
1. Know the length of your telomeres

28. Telomere Testing
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

29. Telomere Testing
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength
Normal Line

30. A Normal 50 Year Old
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

31. Telomeric < Chronological
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

32. Telomeric > Chronological
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

33. Accelerated Aging
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

34. Progeria
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

35. Always Hope to Be
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

36. Never Want to Be
0
2
4
6
8
10
0 20 40 60 80 100
Age (Years)
TelomereLength

37. Telomere Length Measurement
1. Terminal Restriction Fragment (TRF)
2. Q-PCR
3. Fluorescence In Situ Hybridization (FISH)
4. Flow FISH
5. HT Q-FISH
6. Universal STELA

38. Telomere Length Measurement
1. Terminal Restriction Fragment (TRF)
2. Q-PCR
3. Fluorescence In Situ Hybridization (FISH)
4. Flow FISH
5. HT Q-FISH
6. Universal STELA
Short
Telomeres

39. Telomere Length Measurement
1. Terminal Restriction Fragment (TRF)
2. Q-PCR – Commercially available
3. Fluorescence In Situ Hybridization (FISH)
4. Flow FISH – Commercially available
5. HT Q-FISH – Commercially available
6. Universal STELA

40. Telomeres Shorten as Cells Divide
Hastings, et al. Cell Prolif. 37, 317-324 (2004)

41. Telomeres Shorten with Age
Tsuji, et al., Forensic Sci Int 2002

42. Okuda, K., et al., Atherosclerosis, 2000
Telomeres Shorten with Age

43. Weak Correlation?
 People born with different telomere lengths
 Rates of cell division differ between people
 Different environmental factors accelerate
telomere shortening
 Poor Signal Detectability

44. Weak Correlation?
Good for Population Studies
or
Looking at Major Differences
in Individuals

45. Abundance of Short Telomeres?

46. Before
Before
After
After
Small Differences
Easier to Detect!

47. Managing Telomere Length
1. Know the length of your telomeres
2. Decrease the rate of telomere shortening

48. Exercise

49. German National Track and Field Team
Werner, et al., Circulation. 2009 Dec 15;120(24):2438-47
p < 0.001

50. German National Track and Field Team
p < 0.001
Werner, et al., Circulation. 2009 Dec 15;120(24):2438-47

51. Vigorous Aerobic Exercise
LaRocca, et al., Mech Ageing Dev. 2010 Feb;131(2):165-7.

52. Physical Activity in Leisure Time
Cherkas, et al., Arch Intern Med. 2008 Jan 28;168(2):154-8.

53. Stress

54. Stress
p = 0.05
Damjanovic, et al., J Immunol. 2007 Sep 15;179(6):4249-54

55. Stress
Epel, et al., Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17312-5.

56. Stress
Tyrka, et al., Biol Psychiatry. 2010 Mar 5. [Epub ahead of print]

57. Depression

58. Mood Disorders
Simon, et al., Biol Psychiatry. 2006 Sep 1;60(5):432-5.

59. Obesity, Diet,
Antioxidants, &
Smoking

60. 2284 women from the Nurses’ Health Study
Cassidy, et al., Am J Clin Nutr. 2010 Mar 10. [Epub ahead of print]

61. Valdes, et al., Lancet. 2005 Aug 20-26;366(9486):662-4.
p = 0.031 p = 0.019
p = 0.017 p = 0.045
Obesity
Smoking

62. Smoking
p = .001
Morlá, et al., Eur Respir J. 2006 Mar;27(3):525-8.

63. Omega 3 Fatty Acids

64. Omega 3 Fatty Acids
Farzaneh-Far, et al., JAMA. 2010 Jan 20;303(3):250-7.

65. Vitamin D

66. Vitamin D
Richards, et al., Am J Clin Nutr. 2007 Nov;86(5):1420-5.

67. Attitude

68. The MONICA Northern Sweden Study
Self Perceived Aging
Nordfjäll et al., Scand J Public Health. 2008 Sep;36(7):744-52

69. The MONICA Northern Sweden Study
Self Perceived Aging
Nordfjäll et al., Scand J Public Health. 2008 Sep;36(7):744-52

70. The MONICA Northern Sweden Study
Self Perceived Aging
Nordfjäll et al., Scand J Public Health. 2008 Sep;36(7):744-52

71. Pessimism
O’Donovan, et al., Brain Behav Immun. 2009 May;23(4):446-9.

72. Keeping Telomeres Long
 Exercise
 Anti-Oxidants
 Omega 3’s
 Vitamin D3
 Don’t Smoke
 Don’t be Obese
 Reduce Stress
 Reduce Depression
 Reduce Pessimism
 Be Happy!

73. Managing Telomere Length
1. Know the length of your telomeres
2. Decrease the rate of telomere shortening
3. Increase the length of the telomeres

74. Telomerase
Telomere
Telomerase

75. Sierra Sciences, LLC

78. ABC News
Diane Sawyer

80. Bernardes de Jesus B, Vera E, Schneeberger K, Tejera AM, Ayuso E, Bosch F, Blasco MA. EMBO Mol
Med. 2012 Aug;4(8):691-704. Epub 2012 May 15.
Telomerase gene therapy in adult and
old mice delays aging and increases
longevity without increasing cancer.

81. Bernardes de Jesus B, Vera E, Schneeberger K, Tejera AM, Ayuso E, Bosch F, Blasco MA. EMBO Mol
Med. 2012 Aug;4(8):691-704. Epub 2012 May 15.
Telomerase gene therapy in adult and
old mice delays aging and increases
longevity without increasing cancer.

84. Searching for Telomerase
Inducers

85. Reproductive Cells Produce
Telomerase
Telomerase GeneRegulatory
Element

86. Telomerase GeneRegulatory
Element
The Gene is Repressed in All
Other Cells

87. Sierra Sciences, LLC

89. 39 Families of Chemicals
Identified
Human Testing within 3 Years

90. Natural Products Already on the
Market!

91. Clinical Studies

92. Study #1
Not Published

95. Study #2

97. Telomerase Induction In Vitro
Chaps
100%Hela
5%Hela
MRC5DMSO
MRC5Media
0.01uM 0.1uM 1uM 10uM 100uM

98. Abundance of Short Telomeres
Reduced
(Harley, Liu, Blasco, Andrews, Briggs and Raffaele, Rejuvenation Research,
2010)

100. Clinical Study
 Telomerase Induction
 Short Telomeres Lengthened
 CD28- T Cells
 Neutrophils
 Natural Killer Cells

101. Study #3
Completed, but Not Published Yet

102. Clinical Study
 Naive T Cells
 Blood Pressure
 Skin Elasticity
 Bone Density
 Cholesterol
 Homocysteine
 Cognitive Function
 Glucose Levels

103. Clinical Studies
Underway

104. Study #4

105. Clinical Study
 Faces, Hands, etc., Photographed
 Short telomeres measured
 Waist circumference
 Body Mass Index (BMI)
 Total Body Water (TBW)
 Total Body Fat (TBF)
 Oxidative Stress Panel
 Routine Urinalysis

106. Clinical Study
 CBC w/Diff
 Thyroid panel including a Free T3
 HSCRP (Highly sensitive C Reactive
Protein)
 Total and free Testosterone
 Total Estrogen
 Chem 20 including a lipid profile
 IGF-1

107. Study #5

108. Clinical Study
 Double Blind Placebo Controlled
 Short Telomeres Measured

109. The War has
Just Begun!

110. Recent Publications
www.sierrasci.com/RecPubs.html

111. www.sierrasci.com/RecPubs.html

112. QuestionsForBill@yahoo.com
Thank you!