LC-MS

1. Bottom up top down
proteomics techniques

2. Top-down Proteomics can characterize intact
proteins from complex biological systems. This
approach routinely allows for 100% sequence
coverage and full characterization of proteomes,
the specific molecular form of the protein resulting
from combinations of genetic variation, alternative
splicing, and post-translational modifications.
Fragmentation for tandem mass spectrometry is
accomplished by electron-capture dissociation or
electron-transfer dissociation. Proteins are typically
ionized by electrospray ionization and trapped in a
Fourier transform ion cyclotron resonance or
orbitrap mass spectrometer.
Bottom-up proteomics involves in
proteolytic digestion of proteins before
analysis by mass spectrometry. And the term
bottom-up implies that information about the
constituent proteins is reconstructed from
individually identified fragment peptides
released from the protein through
proteolysis.

3. Top-down Proteomics can characterize intact
proteins from complex biological systems. This
approach routinely allows for 100% sequence
coverage and full characterization of proteomes,
the specific molecular form of the protein resulting
from combinations of genetic variation, alternative
splicing, and post-translational modifications.
Fragmentation for tandem mass spectrometry is
accomplished by electron-capture dissociation or
electron-transfer dissociation. Proteins are typically
ionized by electrospray ionization and trapped in a
Fourier transform ion cyclotron resonance or
orbitrap mass spectrometer.
Bottom-up proteomics involves in
proteolytic digestion of proteins before
analysis by mass spectrometry. And the term
bottom-up implies that information about the
constituent proteins is reconstructed from
individually identified fragment peptides
released from the protein through
proteolysis.

4. Top-down Proteomics can characterize intact
proteins from complex biological systems. This
approach routinely allows for 100% sequence
coverage and full characterization of proteomes,
the specific molecular form of the protein resulting
from combinations of genetic variation, alternative
splicing, and post-translational modifications.
Fragmentation for tandem mass spectrometry is
accomplished by electron-capture dissociation or
electron-transfer dissociation. Proteins are typically
ionized by electrospray ionization and trapped in a
Fourier transform ion cyclotron resonance or
orbitrap mass spectrometer.
Bottom-up proteomics involves in
proteolytic digestion of proteins before
analysis by mass spectrometry. And the term
bottom-up implies that information about the
constituent proteins is reconstructed from
individually identified fragment peptides
released from the protein through
proteolysis.