1. PRESENTED BY
Karisma Dash, Reg .No : 1803253041
Sarita Kumari Satapathy, Reg .No :
1803253076
Chapala Mandala, Reg.No:1803253022
Ashit Gain, Reg. No: 1803253014
UNDER THE GUIDANCE OF
MS. ITISHREE NAYAK
Asst. Professor,
Department of pharmaceutics
COLLEGE OF PHARMACEUTICAL SCIENCES
PO/AT: MOHUDA, BERHAMPUR – 760001 ,ODISHA
FORMULATION AND EVALUTION
OF
POLYHERBAL SHAMPOO

2.  INTRODUCTION
 PLANT PROFILE
 AIM AND OBJECTIVE
 PLAN OF WORK
 MATERIALS AND METHOD
 RESULTS
 CONCLUSION
CONTENT

3. DEFINATION:- Shampoo are probably the most widely used cosmetic products for
cleansing hair and scalp in our daily life. Herbal shampoos are the use of traditional
ayurvedic herbs for cleansing the hair and scalp like the regular shampoo.
It should effectively and completely remove dandruff, dust , soil, environmental pollutions,
excessive sebum and loose corneal cells from the hair . It should be easily removed on rinsing
with water.
FUNCTION:- Lubrication, conditioning, hair growth
ADVANTAGES:- No surfactants ,pure and organic ingredients,
no side effects, No animal testing.
BENIFITS:- More shine , less hair loss, won’t irritate skin or scalp.
INTRODUCTION

4. LITERATURE REVIEW
Sutar Manisha et al. formulated a polyherbal shampoo using amla fruit, hibiscus leaf, neem leaf, shikakai fruit, aloe leaf,
henna leaf, Retha fruit and evaluated for organoleptic, powder characteristics, dirt dispersion, wetting time, foam test
and physical evaluation and considered as safe.
Gholamreza Dehghan et al. formulated a herbal conditioner shampoo using fenugreek seeds methanol extract and
evaluated for physicochemical properties. It is concluded that the formulated shampoo has a good quality of introducing
it to the market.
Mohamed Halith et al. formulated herbal shampoo using natural ingredients with tulasi and neem. Both are having anti
dandruff action. The study revealed that the anti dandruff activity of Ocimum sanctum and Azadiracta indica against
strains of G+ and G- organisms and fungal organisms.
Swati Deshmukh et al. formulated a herbal shampoo using aloevera, neem, shikakai, reetha, amla, brahmi and evaluated
and concluded as safe.

5. AMLA
Family:- Phyllanthaceae
Synonyms:- Indian gooseberry
Biological name:- Phyllanthus emblica
Chemical constituents:- Ellagic acid, Gallic acid, Chebulinic acid. It contains
Vitamin C considered important to slow the ageing radicals.
Uses:- Strengthen the scalp and hair
Reduce Hair Loss
Stimulate Hair Growth
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6. SHIKAKAI
Family:- Fabaceae
Synonyms:- Vimala, Bhuriphena
Biological name:- Acacia concinna
Chemical constituent:- Citric acid, Tartaric acid, Lactose
Uses: Cleanses Hair
Add more to the shine
Crubs Hair loss
Prevent Lice, Psoriasis & Scabies
Prevents Split ends
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7. REETHA
Family:- Sapindoceae
Synonyms:- Soap nut, Wash nut
Biological name:- Sapindus mukorossi
Chemical constituents:- Saponins ,Sugar mucilage ,Protein
Uses :- Prevents Dryness.
- Good for hair growth.
- Reetha reduces dandruff.
- Prevents the scalp from lice.
- Reetha increases the silkness of hair.
- Conditions the hair.
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8. HIBISCUS
Family:- Malvaceae
Synonyms:- Cotton rose, Roselle, Rose mallow
Biological name:- Hibiscus rosasinesis
Chemical constituents:- Flavonoids, Steroids, Quinones
Uses:- Stimulate Hair Growth &Lost hair volume
- Conditions hair
- Prevents Baldness(Minoxidil & Finasteride)
- Treat Dandruff & Itchy Scalp
- Prevents Premature greying
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9. VEGETABLE GELATINE
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ROSE OIL
Family:- Bovidae
Synonyms:- Gelatinum, Puragel
Biological name:- Aquilaria
malaccensis lam
Chemical constituents:- Sodium
Sulfate, calcium chloride dihydrate,
Collagens polypeptide
Uses: Gives thickness to hairs
For strengthening of hairs.
Family:- Rosaceae
Synonyms:- Attar of roses, attar
Biological name:- rosa centifolia
Chemical constituents:- citronellol,
geraniol, Nerol, linalool, nonadecane
Uses:- it repairs hair damage
- improves growth of hair
- reduces the dandruff
- gives fragrance to the shampoo.

10. AIM AND OBJECTIVES
"Shampoo is the cleansing preparation of the hair and scalp." Though there are different types of skin cleansers, but the hair
cleansing preparations can be grouped into only one category and are called as shampoo. They are basically water based
products containing mainly surfactants. it’s Primary function is of cleansing the hair of accumulated sebum, scalp, debris and
residues of hair grooming preparations. The herbal shampoo although better in performance and safer than the synthetic ones
will be popular with consumers.
OBJECTIVES
1) To formulate the herbal shampoo.
2) To evaluate the herbal shampoo.
3) The part used for formulation is leaves, fruits and root.
4) To reduce side effects of chemical formulation.
5) To improve hairs texture.

11. Literature review
Selection of Plant Material
Physical Analysis of Plant Material
Authentication of Plant material
Formulation of polyherbal shampoo
Evaluation of polyherbal shampoo
Result and Discussion
PLAN OF
WORK

12. PHYSICAL STUDIES
Physical Appearance:
 The formulations prepared were evaluated in terms of their clarity, foam producing ability and fluidity.
Determination of pH:
 The pH of shampoo solution in distilled water was determined at room temperature by using pH paper.
Moisture content determination:
Moisture Content = W2-W3
% Moisture Content =
𝑊2−𝑊3
𝑊2−𝑊1
𝑋 100
Where,
Weight of Empty Petri dish =W1
Weight of Sample Crude drug= S
Weight of Petri dish with sample drug (W1+S) = W2
Weight of Petri dish with sample drug after complete drying =W3

13. PHYSICAL STUDIES
Ash Value:
2 Gm of powder drug was taken in silicon dish previously ignited and weighed. Temperature was increased by gradually
increasing the heat not exceeding to red colour. After complete burning, ash is cooled and weighed.
Total Ash Content = W3-W1
% Total Ash Content =
𝑊3−𝑊1
𝑊2−𝑊1
X 100
where,
Weight of Empty Crucible =W1
Weight of Sample Crude drug =S
Weight of Crucible with sample drug (W1+S) =W2
Weight of Crucible with Ash =W3

14. PHYSICAL STUDIES
Extraction value:
extractive value = W2-W1
% of extractive value = extractive value X 100
crude drug taken
(5gm)
Where,
Weight of empty Beaker =W1
Weight of Beaker with 25ml extract after drying =W2
Foaming test:
Test tube numbers 1 to 10
Foaming index =1000/a
=1000/9

15. FORMULATION
Retha extract:
 It is prepared by cold maceration method.
 50 gm of Retha powder in 70% ethyl alcohol.
Amla extract:
 50 gm of amla powder in 250 ml water and boil.
Shikakai extract:
 50 gm of Shikakai powder boiled in 250 ml water.
Gelatine solution:
 Boil 50 ml of water and add 1 gm gelatine powder and again boil for 5 minute.
Hibiscus:
 2.5 gm of hibiscus powder in 50 ml water and boil.

16. PROCEDURE
 Weighed all the ingredients according to the formula.
 Decoction of Hibiscus, Reetha , Amla , Shikakai was
prepared in one part of water. Filter it, by using Filter paper.
 Collect filtrate. Mixed to each other of above filtrate with
constant stirring.
 Mixed gaur gum as a thickening agent for maintenance of
consistency of herbal shampoo as like semisolid nature.
 Preservatives and perfume was added lastly.
Sl.no Ingredients Quantity taken for
50 gm
1 Retha extract 50 gm
2 Amla extract 50 gm
3 Shikakai extract 50 gm
4 Hibiscus extract 2.5 gm
5 Methyl paraben 0.25 ml
6 Rose oil Qs
7 Gelatine solution Qs
8 Citric acid Qs

17. EVALUATION RESULTS
Ingredients % Ash value % Extractive value
% Moisture
content value
Amla 9% 24.80% 6.5%
Shikakai 7% 28% 5.5%
Reetha 6.5% 26.40% 6%
Hibiscus 7.5% 32.80% 9%
PHYSICAL EVALUATION

18. EVALUATION RESULTS
ORGANOLEPTIC EVALUATION/VISUALAPPEARANCE
Serial Test Observation
1 Colour Brownish
2 Odour Pleasant
3 Texture Fine and smooth
FOAMING INDEX CALCULATION FOR HERBAL POWDER
T1 T2 T3 T4 T5 T6 T7 T8 T9 T10
0.8 0.7 0.7 0.9 0.5 0.6 0.9 0.8 1 0.8

19. SL. NO. PHYSIC-CHEMICAL
EVALUATION
RESULT
1 pH 5.49
2 Washability Easily washable
3 Solubility Soluble(water)
4 Skin/eye irritation No harmful effect on skin or eye
5 Foaming capacity Good foaming
6 Extractive values
a) Alcohol soluble
b) Water solubility
16% w/w
12% w/w
7 Ash value 4.88%w/w
8 Dirt dispersion Moderate
9 Moisture content Determination 5.01%
10 Wetting time 170 ± 0.04
11 Stability Stable
12 Nature of hair after washes Soft Manageable
SHAMPOO
EVALUATION

20. DISCUSSION
 Medicinal plants used in the formulation of herbal shampoo were found as rich source of
novel drugs. These plants were Ritha , Amla, Shikakai , Hibiscus been reported for hair
growth and conditioning.
 The various quality control parameters were checked. The pH of the shampoo has been
shown to be important for improving and enhancing the qualities of hair, minimizing the
irritation to the eyes and stabilizing the ecological balance of the scalp.
 Though the product is in dry form inspite has wonderful wetting capacity and being dry is
very good for the storage.
 The evaluation parameters like Organoleptic evaluation, General powder Characters,
Physicochemical Evaluation, Cleaning action, foaming, Dirt dispersion, Wetting agent,
Nature of hair after wash was carried out and was found to be within the standard range.

21. CONCLUSION
A survey of global hair care market trends indicates that consumer use of herbal products has significant
increased over the past years. The factors like UV radiations, use of harsh chemical products have direct and
indirect impact on the hair. The present work focuses on the potential of herbal extracts from cosmetic
purposes. Hence we conclude that the formulation of polyherbal shampoo is effective in reducing dandruff
without irritation, less adverse effect and better conditioning effect. Present investigations was carried out to
formulate the herbal shampoo preparations based upon traditional knowledge and to develop few parameters
for quality and purity of herbal shampoo. Nowadays there is strong demand for natural therapies, and this is
increasing in western countries. The herbs which are a cheapest of phytoconstituents are on wheals to attain
their role in polyherbal formulation so as to have synergistic role. Hence we conclude that the polyherbal
formulation of Shampoo is effective in reducing dandruff without irritation, less adverse effect and better
conditioning effect. The awareness and need for cosmetics with herbs in on the rise, as it is strongly believed
that these products are safe and free from side effects. For the treatment of dandruff we have both synthetic and
natural herbal shampoos. But when compared to the chemical based shampoos, herbal based shampoos are
more effective in terms of safety and ease of manufacturing and in the economic point of view they are cheap .

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5. Maffei C, Fossati A, Rinaldi F, et al. Personality disorders and psychopathologic symptoms in patients with
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