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Probiotic effects on fish seminar presentation and literature review (Lina-81405)pptx

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Probiotic effects on fish seminar presentation and literature review (Lina-81405)pptx

  1. 1. Jannatul Ferdush Exam Roll: 81405 MS session: 2020-2021 Reg. No.: 2016-814-260 Fisheries management Branch Department of Fisheries University of Dhaka Aquaculture and Fisheries 6 (2021) 283–288 Contents lists available at ScienceDirect Aquaculture and Fisheries journal homepage: www.keaipublishing.com/en/journals/aquaculture-and-fisheries Effects of dietary supplementation of Lactobacillus plantarum and Bacillus subtilis on growth performance, survival, immune response, antioxidant capacity and digestive enzyme activity in olive flounder (Paralichthys olivaceus) Yangzhen Li , Yingming Yang , Limin Song , Jialin Wang , Yuanri Hu , Qian Yang , Peng Cheng , Jianhai Li Impact factor: 3.69 (2022) 12/10/2022 1
  2. 2. 1. Introduction 2. Materials and Methods 3. Results and Discussion 4. Conclusion Literature Review Presentation Outline 12/10/2022 2
  3. 3. 3 1.Introduction 12/10/2022
  4. 4. In aquaculture, Growth and disease resistance are the main parameters of commercial importance.  Probiotics is a long lasting, less costly and less time consuming solution and widly used in aquaculture due to their beneficial impacts on,  growth performance and health status  enhance innate immune response  suppress pathogenic microorganisms  L. plantarum and B. subtilis can improve growth performance, strengthen immune responses, modulate activities of antioxidant and digestive enzymes in aquatic species (Akhter et al., 2015)  Olive flounder (Paralichthys olivaceus) is a commercially important marine flatfish species in China, South Korea and Japan. 12/10/2022 4
  5. 5. 12/10/2022 The overall objective of the study Research gap  Little information is available on the beneficial effects of L. plantarum and B. subtilis during the whole growth period (from juvenile to commercial size) under commercial rearing environment. To find out the information about probiotics administration strategy for improving quality and productivity in olive flounder. The specific objectives of the study To evaluate the growth performance and survival To investigate the immunological indices, antioxidant ability To evaluate the digestive enzyme activity 1 2 3 12/10/2022 5
  6. 6. 12/10/2022 6 2.Materials and Methods
  7. 7. Feed formulation Fish collection and maintenance Estimation of Growth performance Sample collection (blood, liver, intestine and stomach) Assessment of parameters Overview of materials and methods 12/10/2022 7
  8. 8. 2.1 Diet preparation 8 Diet Basal diet without probiotics Experimental diet supplemented with Lactobacillus plantarum and Bacillus subtilis Composition Percentage(%) Crude protein 45.0 Crude fiver 10.0 Crude fat 3.0 Crude ash 16.0 Calcium 4.0 Lysine 2.5 Total phosphorus 1.5-3.0 Table-1 The proximate composition of basal diet on dry matter basis provided by manufacturer. 12/10/2022
  9. 9. 9 Collection of Olive flounder juveniles( approximately four months old) Fish were fed two times daily Randomly distributed into three groups and each group consists of three replicates Each tank was supplied with filtered and sterilized sea water Water temperature was controlled at 10–24 ⸰C, the salinity at 28–30ppt, the dissolved oxygen at 6–8 mg/L, water exchange rate at 500% per day. 2.2 Fish grow-out maintenance 12/10/2022
  10. 10. According to, (Crane et al, 2020) Weight gain rate (WGR): 𝑾𝟏−𝑾𝟎 𝑾𝟎 × 100% Specific growth rate(SGR): ( 𝒆 𝒍𝒏 𝑾𝟏 −𝒍𝒏 𝑾𝟎 𝑻 −𝟏) × 𝟏𝟎𝟎% Survival rate: 𝑵𝟏 𝑵𝟎 ×100% Where,  W0 is the initial body weight  W1 is the harvest body weight  N1 is fish number at harvest of each tank  N0 is fish number at stocking of each tank  T is days of grow-out period 2.3 Growth performance and Survival rate 12/10/2022 10
  11. 11. 2.4 Sample collection procedure Three starved fish were randomly selected after anesthetized Approximately 1 mL of whole blood samples were drawn from the caudal vein using syringes After clotting the sample at room temperature, Serum was separated by centrifugation (1500 × g, 10 min, 4 ◦C) and stored at -80 ◦C until use Fresh liver, stomach and middle intestine tissue samples were collected and then stored at -80 ◦C 12/10/2022 11
  12. 12. 2.5 The assay of immune ,antioxidant and digestive parameter Immune parameter • Lysozyme (LYS), Acid phosphatase (ACP),Immunoglobulin M (IgM), Complement protein 3 (C3) and Complement protein 4 (C4) Antioxidant parameter • Catalase (CAT), Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px), Total antioxidant capacity (T-AOC) and Malondialdehyde (MDA) Digestive parameter • Proteinase, Amylase and Lipase 12/10/2022 12
  13. 13. 13 50 μL diluted samples (1:4) and 100 μL horseradish peroxidase (HRP) conjugated antibody- enzyme reagent were added Incubated at 37 ◦C for 60 min and then washed with washing solution for 5 times. After drying, tetramethyl benzidine (TMB) chromogenic substrate was pipetted and blended gently Again after incubation at 37 ◦C for 15 min in darkness, the enzymatic reaction was stopped by adding 50 μL stopping buffer Then the absorbance at 450 nm was assayed using a Rayto RT-6100 microplate reader 2.5 Continue… 12/10/2022
  14. 14. 2.6 Statistical analysis All statistical analyses were carried out using SPSS 25.0 software. The results were presented as mean ± standard error (SE). 12/10/2022 14 Normality of all the data and the homogeneity of variances among different groups were analyzed by using Shapiro-Wilk test and Levene’s test respectively. One-way analysis of variance (ANOVA) was performed to determine significant differences in various parameter indices, where significance level was set as P- value < 0.05.
  15. 15. 12/10/2022 15 3.Results and discussion
  16. 16. Table 2 Initial body weight (IBW), harvest body weight (HBW), weight gain rate (WGR), specific growth rate (SGR) and survival rate (SR) of olive flounder. Values are expressed as mean ± SE. Values with different superscript letters in the same row show significant differences (P < 0.05). Index Diet L. plantarum B. subtilis Control IBW (g) HBW (g) WGR (%) SGR SR (%) 18.54 ± 0.71a 370.7 ± 3.77b 1896.06 ± 66.57b 0.99 ± 0.012b 73.89 ± 2.84a 18.22 ± 0.84a 397.50 ± 3.11a 2085.08 ± 64.10a 1.02 ± 0.010a 66.85 ± 3.56ab 18.87 ± 0.89a 353.29 ± 2.76c 1836.72 ±72.85c 0.98 ± 0.014c 61.94 ± 3.68b 3.1. Growth performance parameters and survival 12/10/2022 16  The growth performance parameters were significantly enhanced by dietary supplementation feed than control.  B. subtilis supplementation feed show great superiority for growth improvement in olive flounder.
  17. 17. 3.2. Immunological indices Index Diet L. plantarum B. subtilis Control LYS (U/L) 5.99 ± 0.11a 4.32 ± 0.08b 3.69 ± 0.07c ACP (U/L) IgM (μg/mL) C3 (μg/mL) C4 (μg/mL) 11.13 ± 0.22a 46.94 ± 0.41a 150.27 ± 5.09a 195.21 ± 4.21a 7.95 ± 0.25b 42.02 ± 0.17b 132.51 ± 3.06b 133.23 ± 0.69b 7.81 ± 0.15b 37.62 ±0.58c 98.43 ±0.87c 123.76±2.3b Table3 Effects of L. plantarum and B. subtilis supplementation in diets on immune response. Values are expressed as mean ± SE (n = 9). Values with different superscript letters in the same row show significant differences (P < 0.05). LYS: lysozyme; ACP: acid phosphatase IgM: immunoglobulin M; C3: complement protein 3; C4: complement protein 4.  Serum immunological indices are significantly higher for L. plantarum.  LYS is a bacteriolytic enzyme which is one of the most important serum factors because it can activate the cellular defenses (Harikrishnan et al, 2010). 12/10/2022 17
  18. 18. 3.3. Hepatic antioxidant capacity L. plantarum B. subtilis Control SOD (U/g) 1390.7±57.0b 1590.05±158a 1046.73±93b CAT (U/g) 538.5 ±34.53a 590.22 ± 42.20a 408.45±30.1b GSH-Px(U) TAOC(U/) MDA(U/g) 1.20 ± 0.11a 133.92±11.5a 80.15 ± 2.63b 1.19 ± 0.08a 128.54 ± 15.36a 75.56 ± 2.26b 0.96 ± 0.15a 80.01±16.10b 106.98±5.50a Antioxidant enzymes can remove excessive ROS and protect the cells from lipid peroxidation. The lower value of MDA the better the conditions of liver  Supplemented feed had elevated the activities of SOD,CAT, GSH-Px and T-AOC and lowered contents of MDA in the liver. Table 4 Effects of L. plantarum and B. subtilis supplementation in diets on liver antioxidant indices. Values are expressed as mean ± SE (n = 9). Values with different superscript letters in the same row show significant differences (P < 0.05). SOD: superoxide dismutase; CAT: catalase, GSH-Px: glutathione peroxidase, T-AOC: total antioxidant capacity; MDA: malondialdehyde. 18
  19. 19. 3.4.1. protease enzyme activity Fig. 1. Protease activities in liver, stomach and intestine. Different superscripts indicate significant differences (P < 0.05) between treatments in the same viscus. 19  For stomach and intestine protease activity were significantly higher in L. plantarum and B. subtilis than control.  For liver B. subtilis supplemented feed was higher L. plantarum
  20. 20. 3.4.2. Lipase activity  Lipase activity in dietary B. subtilis supplementation group was substantially (significant in intestine and stomach, and insignificant in liver) higher than L. plantarum group. Fig 2. Lipase activities in liver, stomach and intestine. Different superscripts indicate significant differences (P < 0.05) between treatments in the same viscus. 20
  21. 21. 3.4.3. Amylase activity  In liver the improvement levels of both supplemented feed was negligible.(p>0.05)  In stomach and intestine amylase activity was significantly higher in both supplemeted feed than control.  But L. plantarum activity was not significant than control. Fig. 3. Amylase activities in liver, stomach and intestine. Different superscripts indicate significant differences (P < 0.05) between treatments in the same viscus. 21
  22. 22. 12/10/2022 22 4. Conclusion
  23. 23. Separate supplementation of L. plantarum and B. subtilis in diet is more beneficial for disease resistance (high survival) and growth performance respectively. Feed supplemented with B. subtilis and L. plantarum had a beneficiary effects on innate immune indices, antioxidant and digestive activities. The beneficiary compounded supplementation of L. plantarum and B. subtilis in feed can be a great interest in fish farming industries, feed and additive manufacturers and even aquaculture researchers. 12/10/2022 23
  24. 24. 12/10/2022 24 Thesis title Effects of probiotics on digestive enzymes, antioxidant enzymes and immune defense in Gulsha Tengra (Mystus cavasius)
  25. 25. Received: 5 March 2020 | Revised: 26 October 2020 | Accepted: 29 October 2020 DOI: 10.1111/are.15009  Growth performances, water quality, digestive enzyme and antioxidant enzymes in liver spleen and kidney were improved.  16S rRNA indicated that, modulate gut microbial communities and significantly increase functions relating to nitrogen metabolism, phosphorylation and proteinases. O R I G I N A L A R T I C L E Effects of Clostridium butyricum on growth, digestive enzyme activity, antioxidant capacity and gut microbiota in farmed tilapia (Oreochromis niloticus) Mingqing Zhang1 | Bo Dong2 | Xingxing Lai1 | Zhuang Chen2 | Liping Hou1 | Rui Shu3 | Yanhua Huang4 | Hu Shu1 Key Findings 12/10/2022 25
  26. 26. Probiotics and Antimicrobial proteins (2021) 13:1723–1733 https://doi.org/10.1007/s12602-021-09787-3 The Effect of Lactococcus lactis subsp. lactis PTCC 1403 on the Growth Performance, Digestive Enzymes Activity, Antioxidative Status, Immune Response, and Disease Resistance of Rainbow Trout (Oncorhynchus mykiss) Sakineh Yeganeh1 · Milad Adel2 · Ahmad Nosratimovafagh1 · Mahmoud A. O. Dawood3  Dietary Lactococcus lactis subsp. lactis PTCC 1403 enhanced the feed efficiency, digestive enzyme activity, antioxidative status, and immunity of rainbow trout.  Increased resistance against infection. Key Findings Published online: 1 June 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018, corrected publication June/2018 12/10/2022 26
  27. 27. Aquaculture Nutrition Volume 2022, Article ID 8968494, 17 pages https://doi.org/10.1155/2022/8968494 Hindawi Research Article Effects of Single and Mixture Probiotic Supplements on Growth,Digestive Activity, Antioxidative Status, Immune and Growth- Related Genes, and Stress Response of Juvenile Red Sea Bream (Pagrus Major) Ronick Spenly Shadrack ,1,2 Ishikawa Manabu,2 Shunsuke Koshio,2 Saichiro Yokoyama,2 Yukun Zhang,1 Kumbukani Mzengereza ,1 Mohammed Fouad El Basuini ,3,4 and Mahmoud A. O. Dawood 5,6  Mixed probiotic bacteria strain containing commercial strain has significant potential to improve growth performance, feed utilization, and immune response in red sea bream. Key Findings 12/10/2022 27
  28. 28. Probiotics and Antimicrobial Proteins (2019) 11:545–558 https://doi.org/10.1007/s12602-018-9409-8 Dietary Supplementation of Probiotic Bacillus subtilis Affects Antioxidant Defenses and Immune Response in Grass Carp Under Aeromonas hydrophila Challenge Ying Tang1,2 & Lei Han1,3 & Xiaoxuan Chen1,2 & Mengqi Xie1,2 & Weiguang Kong1,4 & Zhixin Wu1,2 Published online: 13 April 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 Key Findings  Bacillus subtilis CH9 strain, isolated and identified used as a probiotic to protect grass carp against pathogenic strain of Aeromonas hydrophila, and improved fish antioxidant defences and immune response. 12/10/2022 28
  29. 29. Aquaculture 294 (2009) 306–313 Contents lists available at ScienceDirect Aquaculture journal homepage: www.elsevier.com/ locate/aqua-online Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris Mathieu Castex ⁎, Pierrette Lemaire, Nelly Wabete, Liet Chim Key Findings  Probiotic supplementation in shrimps' diet is an effective way to reduce the susceptibility of shrimps to bacterial pathogens.  Oxidative stress reduced in the shrimps fed with the probiotic diet.  Lower the load of Vibrio in the shrimps' haemolymph and higher survival rates. 12/10/2022 29
  30. 30. Aquaculture 16 (2020) 100237 Contents lists available at ScienceDirect Aquaculture journal homepage: www.elsevier.com/ locate/aqrep Mst Rubia Banua,*, Shammee Aktera, Md Reazul Islamb, Md Nurunnabi Mondola, Md Amzad Hossainc Key Findings  The growth performance of gulsa tengra in terms of final body weight, weight gain, percent weight gain, and specific growth rate was significantly higher.  Higher fish survivability, proximate composition.  FCR(food conversion ratio) was lowered in supplemented feed. Probiotic yeast enhanced growth performance and disease resistance in freshwater catfish gulsa tengra, Mystus cavasius 12/10/2022 30
  31. 31. Journal of Oceanology and Limnology Vol. 35 No. 1, P. 89-97, 2017 http://dx.doi.org/10.1007/s00343-016-5130-1 Effects of small peptides, probiotics, prebiotics, and synbiotics on growth performance, digestive enzymes, and oxidative stress in orange-spotted grouper Epinephelus coioides ,juveniles reared in artificial seawater WANG Tao (王涛), CHENG Yongzhou (成永洲), CHEN Xiaoyan (陈晓艳), LIU Zhaopu (刘兆普), LONG Xiaohua (隆 小华) ** Key Findings  Digestive enzyme activities significantly enhanced by the small peptide supplement.  Significantly improved growth rates of grouper under artificial seawater conditions.  Dietary supplements did not improve antioxidant capacity under low-salinity stress conditions. 12/10/2022 31
  32. 32. Received: 3 September 2018 | Revised: 28 January 2019 | Accepted: 9 February 2019 DOI: 10.1111/anu.12904 ORIGINAL ARTICLE Dietary supplementation effects of Pediococcus acidilactici as probiotic on growth performance, digestive enzyme activities and immunity response in zebrafish (Danio rerio) Mojtaba Mohammadi Arani1,2 | Amir Parviz Salati1 | Omid Safari3 |Saeed Keyvanshokooh1 Key Findings  The inclusion of P. acidilactici in the diet is an effective way not only to reduce both the production period and costs, but also to improve fish health.  It is suggest that 4 × 106 cfu of the probiotic P. acidilactici in each gram of the zebrafish diet improved growth performance, digestive enzyme and immune response. 12/10/2022 32
  33. 33. Probiotics and Antimicrobial Proteins (2019) 11:938–945 https://doi.org/10.1007/s12602-018-9421-z Effects of Dietary Clostridium butyricum on the Growth,Digestive Enzyme Activity, Antioxidant Capacity, and Resistance to Nitrite Stress of Penaeus monodon Yafei Duan1 & Jiasong Zhang1 & Jianhua Huang1,2 & Shigui Jiang1 Published online: 1 June 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018, corrected publication June/2018 Key Findings  Clostridium butyricum is a good probiotic and its optimum dosage is 1.0% (w/w) for shrimp aquaculture.  Dietary supplementation with C. butyricum improved the growth and intestine digestive enzyme activity, intestine antioxidant capacity and nitrite stress resistance capacity in Penaeus monodon, with no effects on the shrimp body composition. 12/10/2022 33
  34. 34. Aquaculture 531 (2021) 735852 Contents lists available at ScienceDirect Aquaculture journal homepage: www.elsevier.com/ locate/aqua-online Key Findings Lactic acid bacteria supplementation enhanced the liver and intestine antioxidant status and significant improvement in the digestive enzyme activity.  Increase the generation of probiotic bacteria and decrease the abundance of aquatic pathogens. The positive effects of single or conjoint administration of lactic acid bacteria on Channa argus: Digestive enzyme activity, antioxidant capacity, intestinal microbiota and morphology Yidi Konga, Min Lia,c,d,e, Guosheng Chub, Hongjian Liub, Xiaofeng Shana,c,d,e,Guiqin Wanga,c,d,e, Guanghong Hanf, 12/10/2022 34
  35. 35. Thank You 12/10/2022 35

Notas do Editor

  • This figure shows the protease enzyme activity in liver……
  • This table shows the effects of probiotic on lipase activity in different viscera
  • As can be seen from the figure,
  • In summary,….
    And it can be expected that…
  • Based on this topic, I reviewed many literatures.And Now I am focusing some of these findings…
  • Zhang and his co author in 2020 observed that effects…..
  • Yeganeh and his co other in 2021 assayed …
  • Shadrack et al 2022 revealed the
  • Tang et al 2019 worked on the
  • Castex and his co auther 2009 detect the
  • Banua et al 2020 observed the
  • Tao et al 2017 find out the
  • Arani et al 2019 revealed the
  • Daun et al 2019 worked on the
  • Konga et al 2021 detect the

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