2. CHROMATOGRAPHY
A laboratory technique in which the
components of a sample are separated
based on how they distribute between two
chemical or physical phases, one of which
is stationary and other of which is allowed
to travel through the separation system.
3. CHROMATOGRAPHY
Introduced first by the Russian botanist
Mikhail Semenovich Tswett.
Mixtures of solutes dissolved in a common
solvent are separated from one another by
a differential distribution of the solutes
between two phases.
4. PRINCIPLE
Fractionalism of mixtures of
substances
In the operation of the
chromatogram, a mobile gaseous or
liquid phase is use to wash the
substances to be separated through a
column of a porous material.
5. PRINCIPLE
The rate of migration of the solute
depends upon the rate of interaction
of the solute with the two phases, one
being the mobile phases and the
other stationary phase as the
compounds travel through the
supporting medium.
6. DEFINITION OF TERMS:
Capillary Action – the movement of liquid within
the spaces of a material due to the forces of
adhesion, cohesion, and surface tension.
Retention time-the average time of the
substance that is bound by the stationary phase
and will travel slowly through the column and
exit at some later time.
7. DEFINITION OF TERMS:
Peak-the width of the region that contains each
compound
Viscosity- measure of the resistance of a fluid
which is being deformed by either shear stress or
tensile stress.
VOCs – volatile organic compounds made up of
large group of small organic compounds with
boiling point below 200 oC.
8. DEFINITION OF TERMS:
Stationary phase- fixed phase that is
coated or bonded within the column
Mobile phase-phase that is flowing
through the column and causes
sample components to move towards
the column’s end.
9. DEFINITION OF TERMS:
Partition Coefficient- ratio
of concentrations of a compound in
the two phases of a mixture of
two immiscible
solvents at equilibrium.
Volatility- measure of the tendency of
a substance to vaporize
10. DEFINITION OF TERMS:
Eluate- the separated components
Elution-
Eluent- the “carrier” portion of the
mobile phase.
12. COMPONENTS OF A CHROMATOGRAPH
MOBILE PHASE – A phase that is flowing
through the column and causes sample
components to move toward the column’s end.
STATIONARY PHASE- A fixed phase that is
coated or bonded within the column; it always
remain in the system.
It is responsible for delaying the movement of
compounds as they travel through the column.
SUPPORT- onto which the SP is coated or
attached.
13. COMPONENTS OF A CHROMATOGRAPH
ORIGINAL SAMPLE
AND MOBILE PHASE
COLUMN
SUPPORT AND
STATIONARY
PHASE
Receiving
vessel
16. GAS CHROMATOGRAPHY
A type of Chromatography in which the
mobile phase is a GAS.
First GC system was developed by Erika
Cremer
The presence of a gas mobile phase makes
GC valuable for separating substances like
VOCs that occur naturally as gases that can
easily be placed into gaseous phase.
17. GAS CHROMATOGRAPHY
• It can separate nanograms or
picograms of volatile substances.
• It is principally a method for the
separation and quantitative
determination of gases and volatile
liquids and substances.
18. GAS CHROMATOGRAPHY
HOW IS IT PERFORMED?
A System called Gas Chromatograph is used
to perform GC.
COMPONENTS:
GAS SOURCE
INJECTION SYSTEM
COLUMN
DETECTOR
22. GAS CHROMATOGRAPHY
∞ GAS SOURCE- supplies the mobile phase. It is
typically a gas cylinder equipped with
pressure regulators to deliver the mobile
phase at a controlled state.
∞ TWO STAGE REGULATOR- for the pressure
23. GAS CHROMATOGRAPHY
∞ INJECTION SYSTEM- consists of a heated
loop or port into which the sample is placed
and converted into a gaseous form.
∞ COLUMN- contains the stationary phase and
support material for the separation of
components in the sample.
This column is held in an enclosed area
known as the column oven
24. GAS CHROMATOGRAPHY
∞ COLUMN OVEN- maintains the temperature
at a well-defined value.
∞ DETECTOR- monitors sample components as
they leave the column.
∞ DATA SYSTEM
33. GAS CHROMATOGRAPHY
ELUTION METHODS IN GC:
THE GENERAL ELUTION PROBLEM
x Works well if the sample is relatively simple or
has only a few known compounds.
x Generally used for samples containing only
relatively volatile analytes
x Difficulty to find a single set of conditions that
can separate all the components of a complex
sample with adequate resolution and in a
reasonable amount of time.
34. GAS CHROMATOGRAPHY
ELUTION METHODS IN GC:
Temperature Programming
*vary the temperature of the column over
time.
*temperature increases; retention
decreases
35. GAS CHROMATOGRAPHY
GC SUPPORT MATERIALS
Packed Column
filled with small support particles that acts as an
adsorbent or that are coated with the desired stationary
phase.
36. GAS CHROMATOGRAPHY
GC SUPPORT MATERIALS
Packed Column
Diatomaceous earth is a common support placed on
packed columns. This material is formed from fossilized
diatoms and mainly consists of Silicon dioxide or silica.
37. GAS CHROMATOGRAPHY
Open- Tubular Columns
stationary phase coated on or attached to its interior
surface.
38. GAS CHROMATOGRAPHY
Open- Tubular Columns
A coating of polyimide is present on the outside of
this column to give it better strength and flexibility for
handling and storage.
41. GAS CHROMATOGRAPHY
2. Support- Coated Open-Tubular (SCOT) column
Has an interior wall that is coated with a thin
layer of a particulate support, plus a thin film of a
liquid stationary phase that is coated onto this
support layer.
42. GAS CHROMATOGRAPHY
3. Porous-layer Open-Tubular (PLOT) column
It also contains a porous material that is
deposited on the column’s interior wall, but the
surface of this material is now used directly as the
stationary phase without any additional coating.
44. GAS CHROMATOGRAPHY
◊ Gas-Solid Chromatography
o Solid adsorbent is used as a stationary phase.
o Uses the same material as both the support and
stationary phase, with retention occurring through the
adsorption of analytes to the support’s surface.
o Example of support is a MOLECULAR SIEVE.
o Other supports include:
o ORGANIC POLYMERS - porous polystyrene
o INORGANIC SUBSTANCES – Silica or Alumina
45. GAS CHROMATOGRAPHY
o Increasing the surface area of the GSC support will
increase the phase ratio and result in higher retention for
analytes
o Pore size is important because only compounds smaller
than the pores are able to contact the surface are within
the space.
o Polarity of Support and its functional groups will also
affect how analytes will bind to them.
46. GAS CHROMATOGRAPHY
◊ Gas-Liquid Chromatography
o A chemical coating or layer is placed onto the support
and used as the stationary phase.
o Most Common types of GC.
o 100% dimethylpolysiloxane, 5%phenyl-95% methyl
polysiloxane – Examples of liquids that are used as
Stationary phase.
47. GAS CHROMATOGRAPHY
◊ Gas-Liquid Chromatography
o All of these liquids have High boiling points and low
volatilities, which allows them to stay within the
column at relatively high temperatures that are often
used in GC for sample injection and elution.
o Liquids are also wettable- easy to place onto a support
in a thin, uniform layer.
48. GAS CHROMATOGRAPHY
◊ Bonded Phases
o Column Bleed- most nonvolatile liquid will slowly
vaporize or break apart and leave the column over
time.
o Column bleed changes the retention characteristics of
the column.
o It can also cause some GC detectors to have a high
background and noisy signal as the stationary phase
leaves the column and enters the detector.
o Use of bonded phase minimize column bleed.
49. GAS CHROMATOGRAPHY
◊ Bonded Phases
o Produced by reacting groups on a polysiloxane
stationary phase with silanol groups that are
located on the surface of a silica support.
50. GAS CHROMATOGRAPHY
Types of Gas Chromatography Detectors
General Detectors
x Thermal Conductivity Detector
-used for both organic and inorganic compounds
-measures the ability of the eluting carrier gas and
analyte mixture to conduct heat away from hot-wire
filament-”thermal conductivity”.
-example: Wheatstone bridge
51. GAS CHROMATOGRAPHY
Flame Ionization Detector
- detects organic compounds by measuring
their ability to produce ions when they are burned
in flame.
52. GAS CHROMATOGRAPHY
Selective Detectors
x Nitrogen-Phosphorous Detector
- selective for the determination of nitrogen- or
phosphorous containing compounds.
- Similar to FID, but does not use a flame for ion
production.
53. GAS CHROMATOGRAPHY
Electron capture detector
- detects compounds that have electronegative
atoms or groups in their structure, such as halogen atoms
( I,Br,Cl and F) and Nitro Groups.
-can also be used to detect polynuclear aromatic
compounds, anhydrides and conjugated carbonyl
compounds.
54. GAS CHROMATOGRAPHY/MASS
SPECTROMETRY
A POWERFUL TOOL for both measuring and
identifying analytes as they elute from a GC
column.
Converts a portion of the eluting analytes into
gas-phase ions that can be separated and
detected.
“electron-impact ionization” or “chemical
ionization” can be used to create ions.
56. SAMPLE INJECTION AND
PRETREATMENTT
GAS SAMPLES
Natural candidates for this technique
Should be possible to directly sample and inject
this gas onto a system.
It is often necessary to collect and concentrate
these analytes before they are examined by GC.
Another way of collecting is using a cold trap.
57. SAMPLE INJECTION AND
PRETREATMENTT
LIQUID SAMPLES
Direct injection can be used for a liquid sample
which uses a calibrated microsyringe to apply
the desired volume of sample to the system.
58. SAMPLE INJECTION AND
PRETREATMENTT
Solid samples
Extraction of compounds of interest from the
solid material.
Can be accomplished by using liquid-liquid
extraction or super critical fluid extraction.
59. GAS CHROMATOGRAPHY
ADVANTAGES:
o Ability to provide qualitative information
and quantitative information
o FAST ANALYSIS, HIGH SPEED
o Efficient, providing high resolution
o Sensitive
o Requires small samples
o Inexpensive
o EASY, WELL KNOWN
60. GAS CHROMATOGRAPHY
DISADVANTAGES:
o LIMITED to volatile samples
o Dirty samples require clean up
o Some training/experience is
necessary
o Most use another instrument
for confirmation of Identity (e.g
Mass Spectrometry)
61. GAS CHROMATOGRAPHY
Applications:
Most effectively used for analyses of organic
compounds, space related, complex mixtures of
volatile substances at column temperature of
less than -40 °C to greater than 550° C.
Geochemical research projects such as
determination of various environmental
pollutants at extremely low concentrations.
63. LIQUID CHROMATOGRAPHY
√ A Chromatographic technique in which the
mobile phase is a liquid.
√ Originally developed by Russian botanist Mikhail
Tswett in 1903.
√ Works directly with liquid samples, which makes
it valuable in such areas as food
testing, environmental testing and
biotechnology.
64. LIQUID CHROMATOGRAPHY
√ Components of the LC System:
Support – enclosed in a Column
Stationary phase- enclosed in a Column
Liquid mobile phase-delivered to Column by means of
Pump
Injection device- on analytical applications it is being
used, to apply samples to the column.
Collection Device (optional)- placed after the column
to capture analytes as they elute.
65.
66. LIQUID CHROMATOGRAPHY
FACTORS THAT AFFECT LIQUID CHROMATOGRAPHY:
*requires both a difference in retention and good efficiency
for it to separate two given chemicals
*Sample
*Analyte Requirements
*Column Efficiency
*Role of the Mobile phase
68. LIQUID CHROMATOGRAPHY
1. ADSORPTION CHROMATOGRAPHY
A chromatographic technique that separates solutes
based on their adsorption to the surface of a support.
Also known as Liquid-Solid Chromatography
Equivalent method in GC is Gas-Solid Chromatography
Uses the same material as both stationary phase and
support.
Retention process can be explained on the ff below:
A+ n M-Surface A-Surface + n M
69. LIQUID CHROMATOGRAPHY
Elutropic strength- strength of a mobile
phase in adsorption chromatography
It is a measure of how strongly a particular
solvent or liquid mixture will absorb to the
surface of a given support.
Examples: silica and Alumina supports
A liquid with large elutropic strength will
strongly adsorb to the given support, which
will prevent the analyte from binding to the
support.
70. LIQUID CHROMATOGRAPHY
Stationary Phases and Mobile Phases
Silica (SiO2) - most popular support since it is
polar in nature, thus it will strongly retain polar
compounds.
Alumina (Al2O3) – general-purpose support, but
can retain some polar solutes so strongly that
they are irreversibly absorbed onto its surface.
Carbon-based Compounds- used as nonpolar
supports that retain nonpolar solutes and have a
strong mobile phase that is nonpolar.
71. LIQUID CHROMATOGRAPHY
Applications
Help purify new compounds
Separation of geometrical isomers and
chemicals that belong to a given class of
substances
Remove undesired side-products during
synthesizing AZT or other drugs
73. LIQUID CHROMATOGRAPHY
2. PARTITION CHROMATOGRAPHY
o It is a Liquid Chromatographic technique in
which solutes are separated based on their
partitioning between a liquid mobile phase and a
stationary phase coated on a solid support.
o Support used is usually Silica
o Originally, it involves coating of support with a
liquid stationary phase that was immiscible with
the mobile phase
74. LIQUID CHROMATOGRAPHY
Two Main Categories of Partition Chromatography:
• Normal-phase- uses polar stationary phase
• Reversed phase-uses nonpolar stationary phase
75. LIQUID CHROMATOGRAPHY
Stationary Phases and Mobile Phases
Liquid Stationary Phases coated onto solid supports
Bonded Phases – widely used due to their better stability
and efficiency compared to liquid stationary phases.
Silica - often used as the support. To place bonded phases
on this support, silanol groups on the surface of silica are
first treated with an organosilane that contains the
desired stationary phase as a side chain.
Agents like triethylamine and trifluoroacetic acid can also
be added to the mobile phase to prevent silanol groups
from binding to analytes.
76. LIQUID CHROMATOGRAPHY
Applications:
Used in analytical laboratories
Separation of analytes in organic solvents and
chemicals that contain polar functional groups.
Employed in pharmaceutical industry as a
method for separating and analyzing drugs during
their testing and development.
77. LIQUID CHROMATOGRAPHY
3. ION- EXCHANGE CHROMATOGRAPHY
Solutes are separated by their adsorption
onto a support containing fixed charges on its
surface.
Routinely used in Industry for the removal or
replacement of Ions in products.
Used for the separation of charged compounds
( inorg. Ions, org. ions, AA, Proteins and Nucleic
Acids)
78. LIQUID CHROMATOGRAPHY
Stationary Phases and Mobile Phases
Cation-exchanger – has negatively charged group
and is used to separate positive ions
Anion-exchanger – has positively charged group
and is used to separate negative ions
79. LIQUID CHROMATOGRAPHY
Silica (SiO2) - most popular support since it is
polar in nature, thus it will strongly retain polar
compounds.
Polystyrene – used for small inorganic and organic
ions
Carbohydrate-based Gels – useful in separation of
biological compounds, which can have very
strong, undesirable binding to organic polymeric
resins like polysterene. Example: Agarose
80. LIQUID CHROMATOGRAPHY
Applications
Removal of certain types of ions from samples such as
in water-purification
Direct separation and analysis of samples
83. Factors to be considered in Ion-
Exchange Chromatography
Buffers
pH
Salt Gradient
84. LIQUID CHROMATOGRAPHY
4. SIZE-EXCLUSION CHROMATOGRAPHY
It is a LC technique that separates substances
based on different abilities of analytes to access
the mobile phase within the pores of a support..
o No true stationary phase is present in this system
o Uses a support that has a certain range of pore sizes.
o A separation based on size or molar mass.
85. LIQUID CHROMATOGRAPHY
Stationary Phases and Mobile Phases
Ideal support consists of a porous material
that does not interact directly with the injected
solute.
o Carbohydrate-based supports like dextran and and
agarose in their underivatized form can be used in SEC
for biological compounds and aqueous-based
samples.
o Polyacrylamide gel can also be used
o For organic solvents, Polystyrene can also be used
o Silica containing diol-bonded phase can be used on
aqueous samples
86. LIQUID CHROMATOGRAPHY
APPLICATIONS:
Used in biological samples
Transfer of large analytes from one solution to
another
Removal of salts from sample
Separation of biomolecules and polymers
Estimation of molar mass
87. LIQUID CHROMATOGRAPHY
5. AFFINITY CHROMATOGRAPHY
A Technique based on biologically related
interactions.
o Makes use of the selective, reversible interactions that
characterize most biological systems.
o Example: binding of enzyme with its substrate.
88. LIQUID CHROMATOGRAPHY
Stationary Phases and Mobile Phases
Stationary phase is represented by the
affinity ligand.
o High specificity ligands- bind to only one or a few very
closely related molecules
o Examples:antibodies for binding to foreign agents and
single-stranded Nucleic Acids for separating and binding to
complemetary strands
o General ligands- bind to a family or class of related
molecules
89. LIQUID CHROMATOGRAPHY
Carbohydrate gels like agarose or
cellulose are commonly used with
affinity ligands serve as supports.
Silica can also be used with affinity
ligands
90. LIQUID CHROMATOGRAPHY
APPLICATIONS:
oLarge-Scale Purification method for
enzymes and proteins
oSample preparation
oDirect analysis of complex biological
samples.
oStudy of biological interactions.
92. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
Uses a pressure for the pumping of
aqueous or organic solution through a
column.
The mobile phase is forced under
pressure through a long, narrow
column, yielding an excellent separation
in a relatively short time.
Highly sensitive and specific.
93. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
Become the primary means of
monitoring the use of drugs and of
detecting drug abuse.
Also used to separate the compounds
contributing to the fragrance of the
flowers.
94. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
ADVANTAGES:
An automated process that takes only a few minutes
to produce results.
Uses gravity instead of high speed pump to force
compounds through the densely packed tubing.
Results are of high resolution and are easy to read.
Can be reproduce easily via automated process.
95. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
DISADVANTAGES:
Difficult to detect coelution, which may lead to
inaccurate compound categorization.
High cost for equipment needed to conduct HPLC.
Operation is complex, requiring a trained technician
to operate.
Equipment has low sensitivity to some compounds
because of the speed of the process.
96. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
Applications:
Use in biomedical research, routine
clinical determination and drug
researching programs
97. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
Instruments
Fraction Collector
Auto Sampler
Pumping systems
Columns & Packing
Detectors
Control Data & Processing
99. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
Applications:
Use in monitoring the use of therapeutic
drugs and detecting drug abuse.
also use to separate compounds
contributing to the fragrance of flowers
100. OLD TECHNIQUES IN
CHROMATOGRAPHY
A separation that takes place on a flat
surface or a plane:
PAPER CHROMATOGRAPHY
THIN LAYER CHROMATOGRAPHY
101. PAPER CHROMATOGRAPHY
Based on nature of solvent,
solubility of solute
and rate of diffusion.
Uses paper as the
stationary phase and
a solvent as the
mobile phase.
102. PAPER CHROMATOGRAPHY
Solvent moves
through the paper by
a capillary action
Separation depends
on the solubility of
solute and
solvents, the polarity
of solvent, and
polarity of solutes in
the sample.
104. PAPER CHROMATOGRAPHY
Considered to be the simplest and
the most widely used of the
chromatographic techniques
because its APPLICABILITY TO THE
FOLLOWING:
ISOLATION
IDENTIFICATION
AND QUANTITATIVE
DETERMINATION OF ORGANIC
AND INORGANIC COMPOUNDS
105.
106.
107.
108. THIN LAYER CHROMATOGRAPHY
Used as a semi-quantitative
screening test screening test
Uses as thin layer of silica
gel, alumina gel, polyacrylamide
gel, or starch gel attached to glass
plate as stationary phase and the
mobile phase is liquid solvent.
109. THIN LAYER CHROMATOGRAPHY
Fractions in the sample are generally
quite soluble in the solvent and move
with it up the stationary phase by
capillary action.
Separated fractions are also developed
in TLC by applying a chemical reaction
with the separated fractions to produce
color changes
111. THIN LAYER CHROMATOGRAPHY
ADVANTAGES: DISADVANTAGES:
Simple and economical Spots are often faint
Easy to perform since it
TLC is difficult to
only involves spotting
the stationary phase reproduce
with the sample & Not typically
placing one edge of the automated
stationary phase plate in
the mobile phase
reservoir.
114. General Categories of Chromatographic Methods
Gas Chromatography Type of Stationary Phase
Gas-Solid Chromatography Solid, Underivatized Support
Gas-Liquid Chromatography Liquid-coated support
Liquid Chromatography Type of Stationary Phase
Adsorption Chromatography Solid, Underivatized Support
Partition Chromatography Liquid-coated support or derivatized support
Ion-Exchange Chromatography Support Containing Fixed Charges
Exclusion Chromatography Porous, Inert Support
Affinity Chromatography Support with Immobilized Biological Ligand
115. Prepared by:
Algarne, Den Marion
Artates, Michael Josan
Hosillos, Ianniel Eddyn
Lipardo, Fredrik Thomas
Tapiculin, Jamaica
BMLS3A