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IMViC Biochemical Tests Principle|Indole|Methyl Red|Voges Proskauer|Citrate Test

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IMViC Biochemical Tests Principle|Indole|Methyl Red|Voges Proskauer|Citrate Test

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Bacterial Identification in Microbiology is carried out using 4 main biochemical tests viz. Indole, Methyl Red, Voges Proskauer and Citrate Test. The procedure and principles for each are discussed here.

Bacterial Identification in Microbiology is carried out using 4 main biochemical tests viz. Indole, Methyl Red, Voges Proskauer and Citrate Test. The procedure and principles for each are discussed here.

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IMViC Biochemical Tests Principle|Indole|Methyl Red|Voges Proskauer|Citrate Test

  1. 1. Biochemical TESTs IMViC
  2. 2. INDOLE TEST 01 02 03 04 Test sample/Test culture 2% Tryptone water broth Incubate 370 C for 24 hrs Add 0.5 ml of Kovac’s reagent HCl + p-dimethylamino benzaldehyde in amyl alcohol
  3. 3. PRINCIPLE TRYPTOPHANASE TRYPTONE → INDOLE + PYRUVATE KOVAC’S REAGENT YELLOW CHERRY RED
  4. 4. METHYL RED TEST 01 02 03 04 Inoculate loopful culture in Glucose phosphate broth (MR-VP medium) Incubate at 370 C @ 24 hours 1ml of the broth Add 2-3 drops of methyl red indicator
  5. 5. PRINCIPLE GLUCOSE MIXTURE OF STABLE ACIDS pH RED COLOUR
  6. 6. Vogues Proskauer TEST 01 02 03 04 Inoculate loopful culture in Glucose phosphate broth Incubate at 370 C @ 24 hours 3 ml of 0.5% Alpha Naphthol 3 ml of 40% KOH
  7. 7. PRINCIPLE ACETYL METHYL CARBINOL (ACETOIN) DIACETYL PINKISH RED POLYMER GLUCOSE 𝝰-Naphthol, KOH, O2 Quanidine compounds-Peptones MRVP broth CONDENSE
  8. 8. CITRATE TEST 01 02 03 Simmon’s citrate slant Streak a loopful of culture Incubate at 370 C @ 24 hours
  9. 9. PRINCIPLE Tests bacteria to utilize citrate as sole source of carbon Citrate permease Citrate -----> Pyruvate Ammonium salts Ammonia pH Bromothymol blue Green → Blue

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