Folio Biosciences' tissue microarray product line enhances research by providing multiple patients on one array. This presentation goes into detail on how the microarrays are made and Folio's capabilities in this area.

1. Tissue
ro arrays
Mic

2. Folio Tissue
Array
Human Cancer Tissue Microarray
0.6 mm, 240 spots
Human Alzheimer’s Tissue Macroarray
6mm, 8 spots

3. Tissue Micro Arrays
Quality Control & Assurance
SOP:
• Fully qualified tissues for studies and assays
• Sample cores selected by Board-certified Pathologists
• Every Array slide is individually read and quality checked
• Every 25th slide from Array block stained and read by Pathologist
• Spot size & % retention assessed (95% assured)
• Quality control & verification of pathological data associated with each sample

4. Tissue Arrays
Top 5 problems with TMA
• Poorly fixed tissues & fixative/embedding medium not
optimized for detecting target molecules of interest
• Detachment of specimens while processing
• Heterogeneity of tissue samples
• Sectioning artifacts & lack of batch-to-batch consistency
• Access to relevant clinical data
Data from secondary research

5. Tissue Array Manufacturing
Process
Slide from University of Michigan

6. Tissue Array Manufacturing
Sample Size & Mapping donor Tissue
Slide
from
NCI

7. Tissue Array Manufacturing
Slide from NCI

8. Tissue Array Manufacturing
Donor Block
Slide from NCI

9. Tissue Array Manufacturing
Recipient Block
0%
Slide from NCI

10. Folio Arrays – ISH Result
Neuritic Plaques
Human Liver Cancer & Normal Adjacent tissue

11. Phylogeny Tissue Array
Alzheimer’s Brain Validation Data Images
Neuritic Plaques 100x Neuritic Plaques 400x
Neurofibrillary Tangles 400x

12. Folio Arrays – IHC Result
Alzheimer’s Parietal Cortex Normal Parietal Cortex
(Amyloid Plaques seen in red color) (Amyloid Plaques absent)

13. Tissue Array Manufacturing
Process
Tissue Grossing
Preparation of Donor block
Donor block sectioning
IHC
H & E Staining
(Immunohistochemistry)
Pathologist Evaluation
ISH
(In Situ hybridization)

14. Tissue Array Design
1 2 3 4
Representative tissue A BrTu BrTu BrTu BrTu
B BrNo BrNo BrNo BrNo
• Disease vs Normal
C ColTu ColTu ColTu ColTu
• Total donors D ColNo ColNo ColNo ColNo
E KidTu KidTu KidTu KidTu
• Spot size
F KidNo KidNo KidNo KidNo
• Spot number G OvTu OvTu OvTu OvTu
• Duplicates/ H OvNo OvNo OvNo OvNo
triplicates I LuTu LuTu LuTu LuTu
J LuNo LuNo LuNo LuNo
• Section thickness
K LivTu LivTu LivTu LivTu
L LiVNo LivNo LivNo LivNo
# Coordinates Sex Age Tumor Type Donor Pathological Diagnosis
135 G 4 F 66 Ovary Tumor OV-218 Ovary:mucinous borderline (low malignant potential) tumor with areas of intraglandular
carcinoma
135 G 19 F 53 Ovary Tumor OV-803 Ovary: Serous carcinoma, bilateral, tumor grade high, nuclear 2 to 3/3)
136 G 14 F 64 Ovary Tumor OV-588 Ovary: Adenocarcinoma grade 2, moderately differentiated