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Flow Cytometry
[object Object],[object Object],[object Object],[object Object],Flow Cytometry Is A Powerful Technique For Characterizing Immune Cells
Principles Of Flow Cytometry
How Is It Done:  http://biology.berkeley.edu/crl/flow_cytometry_basic.html
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Limitations With Light Scattering
FSC vs SSC Dot Plot
Fluorescence And Antibodies  To The Rescue
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Fluorescent Dyes And Antibodies
Principles Of Fluorescence E= h f     = f  
Excitation Spectra Of Fluorochromes
Emission Spectra
Architecture Of A FacsCalibur Instrument
Analyzed Data
ISOTYPES Unstained
GM-CSF GM-CSF + IL-4  (6 Days Old)
20 80 GM-CSF+IL-4+PGE 2 1 99 99 1 GM-CSF+IL-4 75 25 GMF: 11.82 GMF: 168.92 GMF: 391.83 GMF: 1.85 Fig. 2

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Flow cytometry