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Flow cytometry
1.
Flow Cytometry
2.
3.
Principles Of Flow
Cytometry
4.
How Is It
Done: http://biology.berkeley.edu/crl/flow_cytometry_basic.html
5.
6.
FSC vs SSC
Dot Plot
7.
Fluorescence And Antibodies
To The Rescue
8.
9.
Principles Of Fluorescence
E= h f = f
10.
Excitation Spectra Of
Fluorochromes
11.
Emission Spectra
12.
Architecture Of A
FacsCalibur Instrument
13.
Analyzed Data
14.
ISOTYPES Unstained
15.
GM-CSF GM-CSF +
IL-4 (6 Days Old)
16.
20 80 GM-CSF+IL-4+PGE
2 1 99 99 1 GM-CSF+IL-4 75 25 GMF: 11.82 GMF: 168.92 GMF: 391.83 GMF: 1.85 Fig. 2
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