O slideshow foi denunciado.
Utilizamos seu perfil e dados de atividades no LinkedIn para personalizar e exibir anúncios mais relevantes. Altere suas preferências de anúncios quando desejar.
New insights into
exercise-induced
Mitochondrial Adaptations
Mitochondria
Cristae
Outer
Membrane
Matrix
Inner
Membrane
Lowell BB, and Shulman GI. Science 307: 384-387, 2005.
Defects ...
Exercise-induced changes in mitochondrial
content and mitochondrial respiration
Does antioxidant supplementation promote
o...
DefinitionsISEAL
Miller, K.L. (2012). Am J Physiol . 302: E496-499.
• Exercise: one-bout of exercise
• Training: a series ...
Mitochondrial adaptationsISEAL
Granata et al. 2016. FASEB J. 30(2): 959-970
TerminologyISEAL
Bishop, D.J., Skinner, T. (2014). Lactate Threshold.
In: “ESSA student manual for Health, Exercise and Sp...
0
2
4
6
8
10
12
14
16
18
20
[lactate]
Treadmill Speed (km/h)
8.5 10 11.5 13 14.5 16 17.5 19 20.5
L1
L2
L3
L4
L5
(HIT)
LI
L...
0
5
10
15
20
25
50 55 60 65 70 80 90 100
% VO2max
[lactate]
High LT
Low LT
L3
L3L2
L5
TerminologyISEAL
Bishop, D.J., Skinn...
TR TR UTUT
TerminologyISEAL
Baldwin et al. (2000). MSSE. 32:1648–1654.
Mitochondrial changes – cross-sectionalISEAL
Bishop et al. (2014). BBA. 140(4): 1266-1275.
Changes in CS (rats) – trainingISEAL
R² = 0.0063
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60
CitrateSynthaseChang...
Changes in CS – trainingISEAL
Training intensity Training volume
R² = 0.797
0
10
20
30
40
50
60
70
80
0 50000 100000 15000...
Changes in CSISEAL
Granata et al. 2016. FASEB J. 30(2): 959-970
Changes in CSISEAL
Training intensity
Granata et al. 2016. FASEB J. 30(2): 959-970
Changes in CSISEAL
Training volume
* Significantly different (P < 0.05) from NT, † from RT
Granata et al. (2016). FASEB J....
Changes in CSISEAL
* Significantly different (P < 0.05) from pre-NT, # from post-NT
Training volume
Granata et al. (2016)....
Changes in complexesISEAL
Granata et al. (2016). FASEB J. In Press
Changes in CS (humans) – trainingISEAL
Training intensity Training volume
R² = 0.797
0
10
20
30
40
50
60
70
80
0 50000 100...
Changes in MR (mice) – trainingISEAL
Training intensity Training volume
R² = 0.7421
0
5
10
15
20
25
30
35
40
45
0 10 20 30...
ISEAL
Granata et al. (2015). FASEB J. in press
Changes in mitochondrial respiration
ISEAL Intensity & Mito Respiration
Daussin, et al. (2008). Am J Physiol. 295: R264-272.
↑36%
↑13%
ISEAL Changes in mitochondrial respiration
Granata et al. (2015). In Review
p160
RIP
140
v
PGC-1α mRNACREBMEF2 PGC-1α
AMPATP
AMPK
P AMPK
P
PGC-1α
MEF2
Mito
Genes
Nucleus
PGC-1α
PGC-1α
p160
ROS p38 M...
ISEAL
Granata et al. (2016) unpublished data
PGC-1α & p53 mRNA
ISEAL
Edgett et al. (2013) PLOS ONE. 8(8): e71623
PGC-1α mRNA
ISEAL
Edge et al. (2015) PLOS ONE. 10(12):e0141317
10 x 2 min
intervals
(90% VO2max, :
1 min rest).
Percival et al. (2015)...
Nuclear PGC-1αISEAL
Granata et al. (2016). Unpublished
Effect of training statusISEAL
Granata et al. (2016). Unpublished
ISEAL
p53 M
M
MDM2
PHF20
p53 M
M
nucleus
cytosol
stress
degradationstability
MDM2 MDM2
PHF20actiononp53
Cui et al. (2012)....
ISEAL P53 and PHF20
Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924.
ISEAL
p53
P
nucleus
cytosol
stability
AMPK
P
p38
P
P53 and PHF20
Granata et al. (2016). Unpublished
ConclusionsISEAL
• Exercise training volume key for
mitochondrial content (citrate synthase)
• Exercise and training inten...
ISEAL Thanks
@BlueSpotScience
David.Bishop@vu.edu.au
Is there an optimal exercise stimulus for mitochondrial adaptations?
Próximos SlideShares
Carregando em…5
×

Is there an optimal exercise stimulus for mitochondrial adaptations?

7.117 visualizações

Publicada em

My talk from our symposium "New insights into exercise-induced Mitochondrial Adaptations" at the acsm meeting in Boston, 2016

Publicada em: Ciências
  • Seja o primeiro a comentar

Is there an optimal exercise stimulus for mitochondrial adaptations?

  1. 1. New insights into exercise-induced Mitochondrial Adaptations
  2. 2. Mitochondria Cristae Outer Membrane Matrix Inner Membrane Lowell BB, and Shulman GI. Science 307: 384-387, 2005. Defects  o Parkinson’s o Alzheimers’s o Huntington’s o Artherosclerosis o Cardiomyopathies o Diabetes ADP + O2  ATP
  3. 3. Exercise-induced changes in mitochondrial content and mitochondrial respiration Does antioxidant supplementation promote or impede skeletal muscle mitochondrial biogenesis? Autophagy and exercise-induced mitochondrial biogenesis Overview
  4. 4. DefinitionsISEAL Miller, K.L. (2012). Am J Physiol . 302: E496-499. • Exercise: one-bout of exercise • Training: a series of exercise bouts • Mitochondrial biogenesis: • the making of new mitochondrial components • it has been suggested that only measurements of the synthesis rates of mitochondrial proteins are indicative of mitochondrial biogenesis
  5. 5. Mitochondrial adaptationsISEAL Granata et al. 2016. FASEB J. 30(2): 959-970
  6. 6. TerminologyISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”. 0 2 4 6 8 10 12 14 16 18 20 8.5 10 11.5 13 14.5 16 17.5 19 20.5 Treadmill Speed (km/h) [lactate] Lactate inflection Lactate threshold
  7. 7. 0 2 4 6 8 10 12 14 16 18 20 [lactate] Treadmill Speed (km/h) 8.5 10 11.5 13 14.5 16 17.5 19 20.5 L1 L2 L3 L4 L5 (HIT) LI LT L6 (SIT) Wpeak Terminology - LTISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”.
  8. 8. 0 5 10 15 20 25 50 55 60 65 70 80 90 100 % VO2max [lactate] High LT Low LT L3 L3L2 L5 TerminologyISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”.
  9. 9. TR TR UTUT TerminologyISEAL Baldwin et al. (2000). MSSE. 32:1648–1654.
  10. 10. Mitochondrial changes – cross-sectionalISEAL Bishop et al. (2014). BBA. 140(4): 1266-1275.
  11. 11. Changes in CS (rats) – trainingISEAL R² = 0.0063 0 10 20 30 40 50 60 70 80 90 100 0 10 20 30 40 50 60 CitrateSynthaseChange(%) Training Intensity (m/min) R² = 0.8753 0 10 20 30 40 50 60 70 80 90 100 0 5000 10000 15000 20000 25000 CitrateSynthaseChange(%) Training Volume (m/wk) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  12. 12. Changes in CS – trainingISEAL Training intensity Training volume R² = 0.797 0 10 20 30 40 50 60 70 80 0 50000 100000 150000 200000 250000 300000 ChangeincitrateSynthaseactivity(%) Training Volume R² = 0.1097 0 10 20 30 40 50 60 70 80 0 50 100 150 200 250 300 Changeincitratesynthaseactivity(%) Training Intensity (%VO2max) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  13. 13. Changes in CSISEAL Granata et al. 2016. FASEB J. 30(2): 959-970
  14. 14. Changes in CSISEAL Training intensity Granata et al. 2016. FASEB J. 30(2): 959-970
  15. 15. Changes in CSISEAL Training volume * Significantly different (P < 0.05) from NT, † from RT Granata et al. (2016). FASEB J. In Press
  16. 16. Changes in CSISEAL * Significantly different (P < 0.05) from pre-NT, # from post-NT Training volume Granata et al. (2016). FASEB J. In Press
  17. 17. Changes in complexesISEAL Granata et al. (2016). FASEB J. In Press
  18. 18. Changes in CS (humans) – trainingISEAL Training intensity Training volume R² = 0.797 0 10 20 30 40 50 60 70 80 0 50000 100000 150000 200000 250000 300000 ChangeincitrateSynthaseactivity(%) Training Volume R² = 0.1097 0 10 20 30 40 50 60 70 80 0 50 100 150 200 250 300 Changeincitratesynthaseactivity(%) Training Intensity (%VO2max) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  19. 19. Changes in MR (mice) – trainingISEAL Training intensity Training volume R² = 0.7421 0 5 10 15 20 25 30 35 40 45 0 10 20 30 40 50 VmaxChange(%) Training Intensity (m/min) R² = 0.1364 0 5 10 15 20 25 30 35 40 45 0 2000 4000 6000 8000 10000 12000 14000 VmaxChange(%) Training Volume (m/wk) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  20. 20. ISEAL Granata et al. (2015). FASEB J. in press Changes in mitochondrial respiration
  21. 21. ISEAL Intensity & Mito Respiration Daussin, et al. (2008). Am J Physiol. 295: R264-272. ↑36% ↑13%
  22. 22. ISEAL Changes in mitochondrial respiration Granata et al. (2015). In Review
  23. 23. p160 RIP 140 v PGC-1α mRNACREBMEF2 PGC-1α AMPATP AMPK P AMPK P PGC-1α MEF2 Mito Genes Nucleus PGC-1α PGC-1α p160 ROS p38 MAPK N R Fp38 P P PThr Thr HDAC P PSe Se NFAT HDAC RIP140 p160 P CaMK P Calcineurin NFAT P T tubule Muscle fibre Action potential Axon terminalAch Motor end plate Na+ K+ Ca2+ P p53
  24. 24. ISEAL Granata et al. (2016) unpublished data PGC-1α & p53 mRNA
  25. 25. ISEAL Edgett et al. (2013) PLOS ONE. 8(8): e71623 PGC-1α mRNA
  26. 26. ISEAL Edge et al. (2015) PLOS ONE. 10(12):e0141317 10 x 2 min intervals (90% VO2max, : 1 min rest). Percival et al. (2015) JAP. 119(11): 1303-1312 PGC-1α mRNA
  27. 27. Nuclear PGC-1αISEAL Granata et al. (2016). Unpublished
  28. 28. Effect of training statusISEAL Granata et al. (2016). Unpublished
  29. 29. ISEAL p53 M M MDM2 PHF20 p53 M M nucleus cytosol stress degradationstability MDM2 MDM2 PHF20actiononp53 Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924. P53 and PHF20
  30. 30. ISEAL P53 and PHF20 Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924.
  31. 31. ISEAL p53 P nucleus cytosol stability AMPK P p38 P P53 and PHF20 Granata et al. (2016). Unpublished
  32. 32. ConclusionsISEAL • Exercise training volume key for mitochondrial content (citrate synthase) • Exercise and training intensity key for mitochondrial respiration) • May be related to exercise-dependent effects on nuclear PGC-1α and p53
  33. 33. ISEAL Thanks @BlueSpotScience David.Bishop@vu.edu.au

×