1.
Athar Hussain
Roll No.2015
BS(Hon)
Bioinformatics and Biotechnology
Govt.College University Faisalabad
Email: Atharmutahari@gmail.com

2.
Construction of Evolutionary tree Using MEGA

3.
MEGA, Molecular Evolutionary Genetics Analysis, is a
freely available software for conducting statistical analysis of
molecular evolution and for constructing phylogenetic trees.
MEGA is an integrated tool for conducting :
• Sequence alignment,
• Inferring phylogenetic trees,
• Estimating divergence times,
Introduction

4.
Conti……..
• Estimating rates of molecular evolution,
• Inferring ancestral sequences,
• Testing evolutionary hypotheses.
MEGA is used by biologists in a large number of laboratories for
reconstructing the evolutionary histories of species and inferring the extent and
nature of the selective forces shaping the evolution of genes and species

5.
Creation Of New Alignment

6.
# Select Types of Sequence to be Align

7.
Sequence Retrieving

8.
Sequence Alignment Using ClustalW

9.
ClustalW Parameters Setting

10.
Multiple Sequence Alignment

11.
Export Alignment to MEGA Format

12.
Save MEGA format File

13.
Construction Of Phylogenetic Tree Using
Maximum Likelihood Method

14.
Opening MEGA format File which we Saved

15.
Parameter Setting For Phylogenetic Tree

16.
Progressing Tree

17.
Rectangular Phylogenetic Tree
Showing the Conserved
Residues

18.
Circular Tree Construction
From Rectangular Tree

19.
Final Circular Phylogenetic
Tree After Grouping Genes
Different color represents
different Groups