1. Plant Research Reagents

2. Introduction
• In the field of plant science, various studies such as the physiological
actions, morphogenesis, and cultivation techniques of plants have
been investigating.
• Tokyo Chemical Industry(TCI) offer a wide range of products used in
plant research, such as plants clearing reagents and plant hormones.

3. Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation: iTOMEI
• Improved TOMEI (iTOMEI) is a method that specializes TOMEI 1) for fluorescent protein observation by Prof. Sakamoto et al. This
method enables simple transparency and allows for clear detection of fluorescent proteins.2,3,4)
We offer suitable reagents for iTOMEI.
Figure 1. Optical sections images of leaf of Arabidopsis thaliana obtained using confocal microscope. Figure 2. Cleared Arabidopsis thaliana by iTOMEI
(left) Non-treatment, (right) iTOMEI treatment
Products
Decoloring solution: T3940 Tissue-Clearing Reagent iTOMEI-D [for Plants]
Mounting solution: T4003 Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]

4. Advantages
• Enables transparency by simple osmosis in just a few days (more than 2 days).
• Keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
• Suppresses autofluorescence.
• Applicable to wide plant species including Oryza sativa, Arabidopsis thaliana, Marchantia polymorpha.
Video for how to use iTOMEI reagents
Please follow the link : https://youtu.be/DM9IxPqhpIs

5. Rapid Transparency Reagent for Plants: TOMEI
TOMEI is clearing method suitable for observing only fluorescent staining dyes. It enables transparency in just a few hours (2 - 3
hours).
Product
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
*T3530 is unavailable except in Japan, U.S. and Europe

6. Plant Growth Regulators
A typical example of plant growth regulator is a plant hormone. Plant hormones are the collective term for compounds produced in
minute amounts by plants to regulate their own physiological functions. To date, seven natural plant hormones have been discovered:
auxins, cytokinins, ethylene, jasmonates, abscisic acid, gibberillins and brassinosteroids (Figure 3).
Figure 3. Natural plant hormones

7. Auxins
Historically, auxins were first discovered as substances which showed phototropism. To date, it has been revealed that they play
numerous roles such as initial development, budding, root growth development, growth of flower parts and cell division. Natural
auxins are 3-indoleacetic acid, 3-indolebutyric acid and phenylacetic acid. Some unnatural synthetic compounds also exhibit the same
activities.
I0022 IAA
I0023 K-IAA
M2605 IAA Methyl Ester
E0878 IAA Ethyl Ester
I0024 IAN
I0026 IBA
I0032 IPA
N0005 NAA
N0007 Na-NAA
N0006 K-NAA
N0624 1-Naphthaleneacetamide
N0045 NOA
B2746 4-BPA
C0250 4-CPA
C0940 PCIB
C0206 MCPA
E1149 MCPB Ethyl Ester
D0396 2,4-D
D1319 Na-2,4-D Monohydrate
O0518 2,4-D Meptyl Ester
T1509 2,4,5-T Potassium Salt
T0451 TIBA
D1942 Dichlorprop
D4800 Dicamba
M3141 cvxIAA
A3390 5-Adamantyl-IAA

8. Cytokinins
Cytokinins are regarded as substances which stimulate cell division, shoot initiation and bud formation, when addition auxins are
added. Typical structure features are adenine with an isopentenyl unit at N6 position, or with an isopentenyl unit with the methyl
terminus being hydroxylated.
A0149 Adenine
A0151 Adenine Sulfate Dihydrate
B1088 N6-Benzyladenine
K0009 Kinetin
Z0012 trans-Zeatin
C0031 Carbanilide
R0079 Forchlorfenuron

9. Abscisic Acids
Abscisic acid is occasionally classified as a sesquiterpene, however, it is biosynthesized from a carotenoid (C40) precursor. It stimulates
the closure of stomata in the absence of water and induces seeds to synthesize storage proteins. It is also released when a plant
experiences stress, as in lack of nutrition, pests, root distress, or disease.
A1698 (S)-(+)-Abscisic Acid

10. Jasmonates
Jasmomates have a distinct fragrance and are biosynthesized from linolenic acid, an unsaturated fatty acid. They inhibit growth in
adverse conditions, and stimulate tuber formation. They promote senescence of leaves, suppression of fruit growth, and the
induction of tuber formation in potatoes.
J0004 Jasmonic Acid (mixture of isomers)
M1068 Methyl Jasmonate (mixture of isomers)
D3225 Dihydrojasmonic Acid
D1431 Methyl Dihydrojasmonate (cis- and trans- mixture)

11. Gibberellins
C1456 Ethephon
Ethylene (Precursor)
G0029 Gibberellin A3