1.
Tissue processing
PRESENTED BY ANIKET CHOUDHARY

2.
DEFINATION OF TISSUE PROCESSING
Tissue processing is the
technique by which fixed
tissue are made suitable for
embedding within a
supportive medium such as
paraffin.

3.
TISSUE PROCESSING CONSIST OF SOME STEPS :-
1. FIXATION
2. DEHYDRATION
3. CLEARING
4. INFILTRATION
5. EMBEDDING
6. BLOCK MAKING
7. SECTION CUTTING
8. ROUTINE STAINING
9. MOUNTING

4.
1. FIXATION
 Fixation is a chemical process by which biological
tissue are preserved and hardening of the organ
in such a way that its original form is retain and
its constituents do not spread out.
 Commonly used fixative in lab
 10% formalin
 Formal saline
 10% buffered formaline
 Alcoholic formaldehyde

5.
2. DEHYDRATION
Dehydration is a process of removal of
water from tissue.
Alcoholic remove unbound water from
tissue sent to a alcohol 70%, 80%, 95%,
ethanol , methyl and action can be used.

6.
3. CLEARING
Zylene is commonly used for clearing a
tissue.
Zylene is helpful in removal of alcohol it
fixed tissue.
Zylene, toluene, benzene, chloroform can
be as a clearing agent.

7.
4. INFILTRATION
 Infiltration is the process why which clearing
agent is completely remove from the tissue.
 Clearing agent is estimated from the tissue by
defusion in the surrounding.
 The process when be added the melted
paraffin wax in tissue is called the infiltration.

8.
5. EMBEDDING
 The principal of embedding is to accurately
and presively oriented a histological sample of
the tissue into a block of paraffin wax.
 To L-mould are used for making a block will
allow the support and for to hold the tissue so
that find cutting and thick section will be cut
easily in a microtomes.

9.
6. BLOCK MAKING
 Metals L-moulds smeared with paraffin wax
 Orient sample so that intend the cutting surface is
preserved against base.
 Fill the moulds with fresh metal wax.
 Place identifying label.
 When block completely solidity remove mould.
 Block ready for cutting.

10.
7. Section cutting
Section cutting by using the microtome:-
Fix the block holder of the microtome.
Insert tightly a knife in the knife holder
with proper position.
Cut the sufficient section.

11.
8. ROUTINE STAINING
“H” and “E” staining can be
used as a routine stain.
“H” and “E” means
Hematoxyline and Eosin.

12.
9. MOUNTING
Mounting by using DPX.
Help to keep a sample in plays during
imagine.
Prevent the specimen from drying out.
Preserve sample over time for long term
storage.

13.
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