THIN PREP
LIQUID BASED PAP TEST
2ND YEAR MLT GIRLS

The Pap test is a valuable
screening test for cervical
cancer and its precursors.

USE OF TEST
• EVALUATION OF CERVICAL-VAGINAL SPECIMEN FOR
PREMALIGNANT, MALIGNANT AND INFLAMMATORY
CHANGES. INCLUDES EVALUATION OF THE CERVIX,
ENDOCERVIX, ECTOCERVIX, VAGINA AND VULVA.

COLLECTION TECHNIQUES
BROOM-LIKE DEVICE
BRUSH
SPATULA

BROOM-LIKE DEVICE
Insert the central bristles of the broom into the
endocervical canal deep enough to allow the
shorter bristles to fully contact the ectocervix.
Push gently, and rotate the broom in a
clockwise direction five (5) times.
Immediately and vigorously swirl the cell
contents into the ThinPrep® vial, swirling the
brush around the bottom of the vial at least 10
times for at least 10 seconds.

BRUSH
Insert the central bristles of the brush into the
endocervical canal so that the bottom of the
brush is visible at base of os.
Slowly rotate the brush in a clockwise
direction ¼-½ turn. NOTE that over-rotating
the brush may cause excessive bleeding
leading to an unsatisfactory Pap test.
Immediately and with some force, vigorously
swirl and twirl the brush against the edges of
the ThinPrep® vial at least 10 times for at
least 10 seconds.

SPATULA
Using a plastic spatula, insert the small end of
the spatula into the ectocervix
Rotate the spatula one full turn in a clockwise
direction
Quickly place the spatula into the vial and
forcefully swish in the liquid against the
ThinPrep® vial 10 times for at least 10 seconds

COMPOSITION OF BUFFER
THE PRESERVCYT IS A METHANOL- BASED, BUFFERED
PRESERVATIVE SOLUTION USED TO SUPPORT CELLS DURING
TRANSPORT AND SLIDE PREPARATION.

Cell dispersion
Swirling the sampling device in the preservation solution •
Strong enough to separate debris and disperse mucus, but
gentle enough to have no adverse effect on cell appearance.
Cell Collection
A gentle vacuum is created within the ThinPrep Pap
Test Filter, which collects cells on the exterior
surface of the membrane. • Cell collection is
controlled by the ThinPrep 2000 Processor’s
software that monitors the rate of flow through the
ThinPrep Pap Test Filter.
Cell Transfer
After the cells are collected on the membrane, the
ThinPrep Pap Test Filter is inverted and gently
pressed against the ThinPrep Microscope Slide. •
Natural attraction and slight positive air pressure
cause the cells to adhere to the ThinPrep Microscope
Slide resulting in an even distribution of cells in a
defined circular area.

CHARACTERISTICS
• IT REMOVES CONTAMINANTS SUCH AS DEBRIS AND MUCUS AND BLOOD
• DISPERSES THE CELLS
• GOOD PRESERVATION
• TRANSPORT
• MONOLAYER
• AUTOMATED
• FDA APPROVED
• SIGNIFICANTLY MORE EFFECTIVE THAN CONVENTIONAL PAP SMEAR
• PAP , HPV, GONORRHOEA, CHLAMYDIA AND TRICHOMONAS

THANKYOU