it can be prepared by student (B.Tech Biotechnology, 2nd year, KAHE). If u have any doubts pls clear with this YOUTUBE

1. NAME : B.SENTHIL KUMAR
REG NO : 20BTBT043
SUBJECT: BIOCHEMISTRY

2. Introduction and Definition
 Principles of Drug Action
1. stimulation
2. depression
3. irritation
4. replacement
5. cytotoxic action
 Mechanism of Drug Action
1. enzyme
2. ion channels
3. transporters
4. receptors

3. DRUG
POWER

4. ?

5. Physiological and biochemical
effects of drugs and their MOA at
organ system, subcellular and
macro molecular level

6. What the drug do
How the drug do
Action – effect sequence
Dose – effect relationship

8. CLASSIFIED AS:
1. stimulation
2. depression
3. irritation
4. replacement
5. cytotoxic action

9. Drug selectively enhance the level of activity of specialized
cells
Eg.
Adrenaline HEART
Pilocarpine SALIVARY GLANDS

10. Drug selectively depress activity of specialized cells.
Eg.
Barbiturates CNS
LA Nerve conduction

11. Drug creates selective noxious
effect on less specialized cells

12.  MILD :-
1.stimulate function
 STRONG :-
1. Inflammation
2. Necrosis
3. Corrosion
4. Damage

13. Use of natural metabolites and hormones in
deficiency states.
Eg.
Levodopa Parkinsonism
Insulin DM
Iron Anemia

16. ENZYME
ION CHANNELS
TRANSPORTERS
RECEPTORS

17. - VERY IMPORTANT
- ALL BIOLOGICAL REACTIONS

18. enzymatic activity
ENZYME
STIMULATION
Natural Metabolites
Receptors
and
Secondary Messengers

19. ENZYMATIC REACTIONS
Enzyme
Induction
Enzyme
Inhibition

20. COMPETITIVE NON COMPETITVE

21. Drug Substrate
Enzyme
X X X

22. KM : increased
Vmax : unchanged
Substrate displaces DRUG
Maximum reaction of velocity attained

23.  Eg 1:
physostigmine
neostigmine
acetylcholine cholinesterase
Eg 2:
sulfanomide PABA Bacterial folate synthesis

24. Drug Substrate
X X X
enzyme

25. Methotrecate > DHFA
5000 times
Dihydrofolate reductase
Km
Vmax

26. ENZYME
Lose property
CS AS
KM : unchanged
Vmax : reduced

27. Eg :
LA obstruct Na+ channels ,
Nifedipine block Ca+ channels
Nicorandil opens K+ channels

29. Desipramine NET
neural uptake of
noradrenaline
Fluoxetine neuronal
5HT
transporter

30. Macromolecule / binding site
Located on surface /within effector cells
Serves to recognizes the signal molecules
Initiate response to it
But itself has no other functions