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SNP (Single Nucleotide Polymorphic), SNP mapping, SNP profile, SNP types, SNP analysis by gel electropherosis and by mass spectrometry, SNP effects, single strand conformation polymorphism, SNP advantages and disadvantages and application of SNP profile in drug choice
Single Nucleotide Polymorphism (SNP)
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What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
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Synopsis Introduction Some Facts Types of SNPs SNPs act as gene markers Methods of Detection Techniques to detect SNPs Allelic Specific Cleavage Differential Hybridization Single Base Extension or minisequencing Alternate Methods for Detecting SNPs Mass Spectrometry Microchips SIGNIFICANCE OF SNPs HAPLOTYPE ADVANTAGES Are SNP data available to the public? Some important SNP database Resources CONCLUSION References
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SNP (Single Nucleotide Polymorphic), SNP mapping, SNP profile, SNP types, SNP analysis by gel electropherosis and by mass spectrometry, SNP effects, single strand conformation polymorphism, SNP advantages and disadvantages and application of SNP profile in drug choice
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What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
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Similar to Snp
Single Nucleotide Polymorphism (SNP) Polymorphism is a generic term that means 'many shapes‘. It is the ability to appsear in different form . A single nucleotide polymorphism (SNP) is a DNA sequence variation occurring when a single nucleotide - A, T, C, or G - in the genome differs between members of a species (or between paired chromosomes in an individual). For example, two sequenced DNA fragments from different individuals, AAGCCTA to AAGCTTA, contain a difference in a single nucleotide. For a variation to be considered a SNP, it must occur in at least 1% of the population. CHARACTERISTICS OF SNP • In human beings, 99.9 percent bases are same. • Remaining 0.1 percent makes a person unique. • Different attributes / characteristics / traits • How a person looks, diseases he or she develops. These variations can be: Harmless (change in phenotype) Harmful (diabetes, cancer, heart disease, Huntington's disease, and hemophilia ) Latent (variations found in coding and regulatory regions, are not harmful on their own, and the change in each gene only becomes apparent under certain conditions e.g. susceptibility to lung cancer) TYPES OF SNP NON-CODING REGION A segment of DNA that does comprise a gene and thus does not code for a protein . CODING REGION Regions of DNA/RNA sequences that code for proteins Synonymous A SNP in which both forms lead to the same polypeptide sequence is termed synonymous (sometimes called a silent mutation). Non synonymous If a different polypeptide sequence is produced they are non synonymous . A non synonymous change may either be missense or nonsense, where a missense change results in a different amino acid, while a nonsense change results in a premature stop codon. SNP Applications • Gene discovery and mapping • Association-based candidate polymorphism testing • Diagnostics/risk profiling • Response prediction • Homogeneity testing/study design • Gene function identification
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Single nucleotide polymorphism or SNP (pronounced “snips”), are the most common type of genetic variation among peoples. Each SNP represents a difference in a single DNA building block, called a nucleotide
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Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence of specific DNA sequences on chromosomes. It uses fluorescently labeled probes that bind to complementary DNA sequences on the chromosomes. When the probes are visualized under a fluorescence microscope, they appear as bright spots of light. FISH can be used to detect a wide range of genetic abnormalities, including chromosomal translocations, deletions, and duplications. It can also be used to identify specific genes or gene loci. A single nucleotide polymorphism (SNP) is a variation in the DNA sequence that occurs when a single nucleotide is changed. SNPs are the most common type of genetic variation in humans and other organisms. They are often used as genetic markers to study disease associations, population genetics, and evolution. SNPs can also be used to develop DNA tests for paternity testing, forensic science, and disease diagnosis. Expressed sequence tags (ESTs) are short sequences of DNA that are derived from expressed genes. ESTs are created by randomly sequencing cDNA libraries, which are libraries of DNA that represent the mRNA transcripts of all the genes that are being expressed in a cell at a given time. ESTs can be used to identify new genes, study gene expression, and map genes to chromosomes.
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Fluid Dr 6th Sem
Fluid Dr 6th Sem
Correlation Of Alpha Fetoprotein & Lf Ts In Liver
Correlation Of Alpha Fetoprotein & Lf Ts In Liver
Presentation1
Presentation1
A S S I N G M E N T
A S S I N G M E N T
Dna Mutations
Dna Mutations
Snp
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“ SINGLE NUCLEOTIDE
POLYMORPHISM” PRESENTED BY SAIMA FAZAL BS MT 3 RD YEAR 6 TH SEMESTER
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