The document summarizes research on using dipstick and immunochromatographic strip tests to diagnose various parasitic diseases. It describes developing and evaluating dipstick and strip tests for hydatidosis, schistosomiasis, toxoplasmosis, and trichinosis using crude antigens prepared from each parasite. The assays demonstrated high sensitivity and specificity compared to other tests in clinical and experimental studies. The document recommends applying the dipstick assay in field studies and developing improved immunochromatographic strips.

1. بسم الله الرحمن الرحيم

2. ACKNOWLEDGMENT Prof. Dr. Mohamed Khairy Makled Professor of Parasitology Faculty of Medicine, Ain Shams University Prof. Dr. Mohamed Al-Hussieny Fayad Professor of Parasitology Faculty of Medicine, Ain Shams University Prof. Dr. Abd-Al Magid Mohamed Kamal Professor of Parasitology Faculty of Medicine, Ain Shams University Dr. Magid Mustafa Al-Sherbiny Assistant Professor of Zoology Faculty of Science, Cairo University Dr. Gihan Mustafa Tawfeek Assistant Professor of Parasitology Faculty of Medicine, Ain Shams University

3. APPLICATION & ASSESSMENT OF IMMUNOCHROMATOGRAPHIC STRIPS & DIPSTICKS IN DIAGNOSIS OF SOME PARASITIC DISEASES

12. 4c 4b + Patients with 1. Ascarisis 2. Cysticercosi 3. Alveolar Echin. 4. Onchocerciasis 5. Filariasis (For dipsticks& ICS) 3c 3b + Patients with 1. Ascarisis 2. Cysticercosis 3. Alveolar Ech. 4. Onchocerciasis 5. Filariasis (For dipsticks &ICS) 1c 1b + Patients with 1. Ascarisis 2. Cysticercosis 3. Alveolar Ech. 4. Onchocerciasis 5. Filariasis (For dipsticks & ICS) Control Sera 4b Patients with 1.Hydatidodid 2. Toxoplasmosis 3. Schistosomiasis 4. Amoebiasis 5. Fascioliasis. (For EITB and FAST-ELISA) 3b Patients with 1.Hydatidosis 2. Trichinosis 3.Schistosomiasis 4. Amoebiasis 5. Fascioliasis. (For EITB and FAST-ELISA) Schistosomiasis Patients 1b Patients with 1.Toxoplasmosis 2. Trichinosis 3.Schistosomiasis 4. Amoebiasis 5. Fascioliasis. (For EITB and FAST-ELISA) 4a Trichinosis patients 3a Toxoplasmosis Patients 1a Hydatidosis patients Group 5 Group 4 Group 3 Group 2 Group 1

14. RESULTS

15. Reaction of Hydatidosis Patients, Normal Controls & Sera of other parasitic Diseases in EITB HYDATIDOSIS EITB

16. %positive reactions of sera of hydatidosis patients, patients with other parasitic infections and normal controls with different bands of crude HCF antigen bands in EITB HYDATIDOSIS EITB

17. Reaction of sera of hydatidosis patients, patients with other parasitic diseases and normal controls to crude HCF antigen by FAST-ELISA HYDATIDOSIS FAST-ELISA

18. Reactions of hydatidosis patients, normal controls and heterologous sera to crude HCF antigen in dipstick assay HYDATIDOSIS DIPSTICKS

20. ICS using crude HCF antigen tested in hydatidosis patients, normal control sera, PBS and D.water HYDATIDOSIS ICS

21. HYDATIDOSIS Tests Sensitivity Specificity Efficiency Predictive value +ve PV -ve PV EITB 100% 91.4% 95.1% 89.7% 100% FAST ELISA 96.2% 100% 98.4% 100% 97.2% Dipsticks 100% 91.4% 95.1% 89.7% 100%

22. Reactions of Schistosomiasis patient and normal control sera with S.mansoni specific fraction in EITB Reactions of Schistosomiasis patient and normal control sera with S.haematobium specific fraction in EITB SCHISTOSOMIASIS EITB

23. % positive reactions of sera of schistosomiasis patients and normal controls with different bands of MAMA and HAMA in EITB SCHISTOSOMIASIS EITB

24. SCHISTOSOMIASIS Number and percentage of positive reactions by FAST ELISA using MAMA FAST-ELISA Groups Total number of tested sera No. (%) Positive MAMA-FASTELISA Schistosomiasis patients 30 29 (96.7) Normal control 10 0 (0)

25. Reactions of Schistosomiasis patients and normal controls sera to S.mansoni Gp30 and S.haematobium Gp23 in dipsticks assay SCHISTOSOMIASIS DIPSTICKS

26. ICS using GP30 of S.mansoni SCHISTOSOMIASIS ICS using GP23 specific for S.haematobium ICS

27. SCHISTOSOMIASIS Tests Sensitivity Specificity Efficiency Predictive value (PV) +ve PV -ve PV EITB 96.7% 100% 97.5 % 100 % 90.9% FAST ELISA 96.7% 100 % 97 .5 % 100% 90.9% Dipsticks 96.7% 100 % 97.5 % 100% 90.9%

28. TOXOPLASMOSIS EITB analysis of antibody responses to crude tachyzoite antigen for sera from toxoplasmosis patient, controls, and sera of patients with other parasitic diseases EITB

29. % positive reactions of sera of toxoplasmosis patients, patients with other parasitic infections and normal controls with different bands of crude tachyzoite antigen TOXOPLASMOSIS EITB

30. Reaction of sera of toxoplasmosis patients, patients of other parasitic diseases and normal controls to crude tachyzoite antigen by FAST-ELISA TOXOPLASMOSIS FAST-ELISA

31. Reactions of toxoplasmosis patients’ sera, normal control and heterologous sera in dipstick assay TOXOPLASMOSIS DIPSTICKS

32. ICS using crude tachyzoites antigen of T.gondii TOXOPLASMOSIS ICS

33. TOXOPLASMOSIS Comparison between results of EITB, and FAST-ELISA in toxoplasmosis patients and controls Tests Sensitivity Specificity Efficiency Predictive value +ve PV -ve PV EITB 100% 100% 100% 100 % 100% FAST ELISA 95.5% 94.3% 94.7% 91.3% 97.1%

34. Reaction of trichinosis patients, normal controls, and sera of different parasitic diseases in EITB TRICHINOSIS EITB

35. %positive reactions of sera of trichinosis patients and normal controls with different bands of Trichinella antigen TRICHINOSIS EITB

36. Reaction of sera of trichinosis patients, patients of other parasitic diseases and normal controls to crude larval antigen of T.spiralis by FAST-ELISA TRICHINOSIS FAST-ELISA

37. TRICHINOSIS Reactions of sera of trichinosis patients, normal controls, and heterologous sera to T.spiralis antigen in Dipstick DIPSTICKS

38. TRICHINOSIS % positive reactions of trichinosis patients’ sera, sera of patients with different parasitic diseases & normal control in dipstick assay. DIPSTICKS

39. TRICHINOSIS ICS using the crude T.spiralis larval antigen ICS

40. TRICHINOSIS Tests Sensitivity Specificity Efficiency Predictive value +ve PV -ve PV EITB 100% 100% 100% 100% 100% FAST ELISA 85.7% 85.7% 85.7% 54.5% 96.8% Dipsticks 100% 100% 100% 100% 100%

41. TRICHINOSIS Reactions of experimentally infected mice sera at different time interval post infection to Trichinella antigen by EITB and dipsticks EXP. STUDY EITB DIPSTICKS

45. THANKS