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San Diego Research Ethics Consortium,
Salk Institute for Biological Studies.
Workshop, Parthenogenetic stem cell lines: Ethical considerations.
8 July 2011, La Jolla, CA
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How is Parthenogenesis Done? (ANIMATED)
1. Nikolay Turovets, PhD Director of Research and Therapeutic Development How is Parthenogenesis Done? San Diego Research Ethics Consortium Salk Institute for Biological Studies Parthenogenetic stem cell lines: Ethical considerations 8 July 2011, La Jolla, CA parthenogenesis.eventbrite.com www.turovets.com
2. Parthenogenetic activation is a way to create pluripotent stem cells without disruption of the viable embryo Fertilization Parthenogenetic activation Ca 2+ time fertilization Ionomycin 5 min Ca 2+ time parthenogenetic activation ionomycin 6-DMAP or puromycin 4-5 hours 6-DMAP or puromycin Nikolay Turovets, PhD www.turovets.com
3. Parthenogenetic activation Parthenogenetic activation is a way to create pluripotent stem cells without disruption of the viable embryo Fertilization No life possible Embryonic stem cells Parthenogenetic stem cells Nikolay Turovets, PhD www.turovets.com
4. HLA heterozygous hpSC Immune-matching with donor HLA homozygous hpSC Immune-matching with people other than the donor Two types of human parthenogenetic stem cells (hpSC) Nikolay Turovets, PhD www.turovets.com Recipient Transplantation Recipients cells Recipient Donor Transplantation Donor cells
9. HLA-heterozygous hpSC lines are totally immune-matched with the oocyte donors Nikolay Turovets, PhD www.turovets.com
10. One HLA-homozygous hpSC line can be immune-matched with millions of people Nikolay Turovets, PhD www.turovets.com
11. hpSCs are patented and published by ISCO Nikolay Turovets, PhD www.turovets.com
12. hpSCs respond to well characterized differentiation signals and demonstrate appropriate gene expression dynamics and transitions Nikolay Turovets, PhD www.turovets.com
13. Hepatocytes derived from hpSC demonstrate appropriate functions in vitro and in vivo ICG test PAS test glycogen storage PROD assay/P450 activity in vitro in vivo Nikolay Turovets, PhD www.turovets.com
15. Differentiation capacity of hpSC: Cornea-like structures CK 18-19 DAPI ZO-1 DAPI Nikolay Turovets, PhD www.turovets.com
16. Major Collaborators Hepatocytes Cedars-Sinai Medical Center, LA Jeffrey Fair UC San Francisco Holger Willenbring Definitive endoderm ViaCyte/Novocell Ed Baedge Kevin D’Amour RPE UC Irvine Hans Keirstead Magdalene Seiler Methylation and global gene expression The Scripps Research Institute Jeanne Loring Louise Laurent Neurology University of Wurzburg, Germany Albrecht Muller Immunogenicity studies UC San Diego Ewa Carrier Matthias von Herrath University of Berlin, Germany Hans-Dieter Volk Genetic studies Genesis Genetics Institute Marcus Hughes Nikolay Turovets, PhD www.turovets.com
Notas do Editor
Results of these work were published in two papers of “Cloning and Stem Cells” Journal in this and last years.