This slide shows you about my science project.

1. Anatomy of Some Dicot Plants in Salaya Putthamonthol Nakornpathom Mahidol Wittayanusorn School, Thailand

2. Introduction

4. Introduction Each kind of vegetables has its own specific nutrition and benefit. The vegetables under our study are Chilli ( Capsicum frutescens L. var. frutescens ) , Celery ( Apium graveolens L.) , Parsley ( Erygium foetidum Linn. ) , Dill ( Anethum gaveolens L.) and Bitter Cucumber ( Momordica charantia L.) . All of which are abundant and easy to obtain in the Salaya area and, moreover, the study in the level of tissue of these plants are not widely known

5. Introduction Since all the study in anatomy of plants are always on the basis of tissue of these plants are not widely known. Since all the study in anatomy of plants are always on the basis of tissue level study, the data of this project can be used as the basis of other kinds of studies regarding vegetables, such as the study of the relation between the plants’ structure and the ability to collect antioxidant substance.

6. Materials, Chemicals and Equipments

10. Methods

11. Method Paraffin method Selecting Fixing Dehydration Infiltration embedding Sectioning Affixation Staining Mounting

12. Select and cut plant into square pieces, 0.5 cm in length.

13. Preserve the specimens in Formalin Acetic Acid (FAA) at least 8 Hr.

14. Suck the air out of the specimens with vacuum pump until there isn’t any bubble.

15. Wash them with ethanol 50%, at least 3 Hr. 3 times.

16. Steep in TBA 50% 70% 85% 95% 100% At least 12 Hr. 50% 70% 85% 95% 100%

17. Steep in pure TBA at least 12 Hr. 3 times.

18. Steep in TBA mixed with paraffin oil 1:1 at least 12 Hr. at 60 Celsius degrees. 60 o

19. Steep in paraplast at least 12 Hr. at 60 Celsius degrees. In 3 times. 60 o

20. Eliminate bubble while Embed in paraplast and wait until getting cool.

21. Cut in shape of cubic and stick on wooden block. Then cut with rotary microtome.

22. Mount them with Formalin 3% and haupt’s adhesive and lay on slide warmer at 40 -50 o C. Then keep at room temperature for 5 days.

23. Dip in xylene 5 Minutes Xylene+ethanol ether + ethanol Ethanol 95% Ethanol 70% Ethanol 50% Ethanol 30%

24. Steep in Safranin-O 1 Hr. Then wash with water.

25. Ethanol 95% Ethanol 70% Ethanol 50% Dip in Ethanol 30% 5 Minutes

26. Absolute Ethanol +Xylene Absolute Ethanol Wash with Ethanol 95% Clove oil Drop fast green Steep in Xylene 6 Hr.

27. Cover them with cover glass by Canada Balsam or permount. Then bake them in oven.

28. Clean up the surplus mountant and keep them in the slide box.

29. Examine with microscope and analysis the result.

30. Methods Search for information Select and preserve the plant specimens in FAA Dehydrate the tissue by preserving it in Tertiary Butyl Alcohol (TBA)

31. Methods Preserve the tissue in melting paraplast at 60 o C Embedding Cut it with rotary microtome Stretch the specimens by using 3% formalin and Haupt’s decisive

32. Methods Staining with Safrinin O and Fast green Wash the stain out Mounting with mounting medium (Permount), Drop it and cover it with cover glass

33. Results & Discussion

34. Results Parsley Leaf 40x

35. Results Dill Leaf 100x

36. Results Celery Leaf 40x

37. Results Chilli Fruit 40x

38. Results Bitter Cucumber Fruit 40x

39. Results Bitter Cucumber Fruit 40x

40. Results Bitter Cucumber Fruit 40x

42. Conclusion

43. Plants which people admire to eat their leafs, celery had larger area of dermal tissue than dill and celery, respectively. While the ratio of ground tissue area and vascular tissue area, celery had much value than celery and dill, respectively. In another group that people admire to eat their fruits, chili had larger area of dermal tissue than bitter cucumber. But bitter cucumber had more value of ratio of ground tissue area and vascular tissue area than chili.

44. References Consortium of Thai Medical Schools. 1997. Local Plant. The War Veterans Organization Press, Bangkok C. Brickell. 2003. The Royal Horticultural Society A-Z of GARDEN PLANT volume1: A-J. Dorling Kindersley L imited, London. L. Bremness. 1994. HERBS. Dorling Kindersley Limited, London. L. Bremness. 2000. HERBS. Dorling Kindersley Limited, London. Faculty, Kasetsart University, Bangkok

45. References Prof. Ass. Dr. Prasart Keurmanee. 2006. Plant Tissue Technique. Botany Department, Science Faculty, Kasetsart University, Bangkok R.F. Evert. 2006. Esau’s Plant Anatomy 3rd edition. John Wiley & Sons, Inc., New Jersey. Rost T . L . , M . G . Barbour, C . R . Stocking, and T . R . Murphy . Plant Biology . 2nd edition . Thomson Higher Education, USA .

46. References Sylvia Lee-Huang, Paul L. Huang, Philip L. Huang, Aldar S. Bourinbaiar, Hao-Chia Chen and Hsiang-Fu Kung. 1995. Inhibition of The Integrase of Human Immunodeficiency Virus (HIV) Type 1 by Anti-HIV Plant Proteins MAP30 and GAP31. M.S. thesis, Department of Biochemistry, New York University School of Medicine, New York, NY 10016 ; tAmerican BioSciences, New York, NY 10021 ; INational Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 ; and IlLaboratory of Biochemical Physiology, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD. Faculty, Kasetsart University, Bangkok

47. References Tiamjai Komkris. 2003. Plant Anatomy. Botany Department, Science Faculty, Kasetsart University, Bangkok Urai Jiramonkholkarn. 2004. Indigenous Vegetables 1. Ammarin Printing and Publishing Ltd., Bangkok Urai Jiramonkholkarn. 2004. Indigenous Vegetables 2. Ammarin Printing and Publishing Ltd., Bangkok