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BIOSAFETY LEVEL 4 Presented by : SheetalNarkar Pooja Lad
BIOSAFETY The application of the combination of laboratory practice and procedures, laboratory facilities and safety equipment when working with potentially infectious  micro-organisms
BIOSAFETY LEVEL A biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facility There are mainly 4 biosafety levels
BIOSAFETY LEVEL 4 This level is required for work with dangerous and exotic agents that pose a high individual risk of : ,[object Object]
 agents which cause severe to fatal disease in humans for which vaccines or other treatments are not availableEg.   Lassa fever, Ebola hemorrhagic fever, hemorrhagic diseases
CODE OF PRACTICES The two-person rule should apply, whereby no individual ever works alone. This is particularly important if working in a Biosafety Level 4 suit facility Laboratory protective clothing, head coverings, scrub suits, coveralls, shoe cover etc Respiratory protective equipment
CODE OF PRACTICES  A complete change of clothing and shoes is required prior to entering and upon exiting the laboratory Personnel must be trained in emergency situations like in the event of personnel injury or illness.
LABORATORY DESIGN AND FACILITY  Primary Containment ,[object Object]
  Suit laboratory,[object Object]
Controlled Access Controlled Air System
Decontamination of effluents Sterilization of waste and materials

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Biosafety Level 4

  • 1. BIOSAFETY LEVEL 4 Presented by : SheetalNarkar Pooja Lad
  • 2. BIOSAFETY The application of the combination of laboratory practice and procedures, laboratory facilities and safety equipment when working with potentially infectious micro-organisms
  • 3. BIOSAFETY LEVEL A biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facility There are mainly 4 biosafety levels
  • 4.
  • 5.
  • 6.
  • 7. agents which cause severe to fatal disease in humans for which vaccines or other treatments are not availableEg. Lassa fever, Ebola hemorrhagic fever, hemorrhagic diseases
  • 8. CODE OF PRACTICES The two-person rule should apply, whereby no individual ever works alone. This is particularly important if working in a Biosafety Level 4 suit facility Laboratory protective clothing, head coverings, scrub suits, coveralls, shoe cover etc Respiratory protective equipment
  • 9. CODE OF PRACTICES A complete change of clothing and shoes is required prior to entering and upon exiting the laboratory Personnel must be trained in emergency situations like in the event of personnel injury or illness.
  • 10.
  • 11.
  • 13. Decontamination of effluents Sterilization of waste and materials
  • 14. THANK YOU