22. STOOL EXAMINATION Kato technique Mesh screen Template Hole Remove the template Cellophane soaked by glycerin (clears faeces( Egg count/ g stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni
23. STOOL EXAMINATION Stoll’s technique NaOH 4 g Stool Erlynmeyer flask 56 CC 60 CC Shake well 0.15 CC Egg count/ slide Eggs/1g= Eggs/slideX100 Egg/day=Eggs/1g X stool wt/g in 24 hrs 24 hr stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni
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26. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Aspirates Blood PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
40. INDIRECT IMMUNOLOGICAL METHODS INDIRECT FLUORESCENT ANTIBODY TEST parasite Patient’s serum (?? AB) Anti human AB fluorescein
41. INDIRECT IMMUNOLOGICAL METHODS ELISA OPD OPD Flat bottom plastic micrititre plate Ag Patient’s serum (?? AB) Anti human AB Peroxidase E AB
42. INDIRECT IMMUNOLOGICAL METHODS CFT Ag Patient’s serum (?? AB) complement Anti sheep AB Sheep’s RBC -ve Ab +ve Ab haemolysis No Sheep RBChaemolysis Tube / microplate AB
44. INDIRECT IMMUNOLOGICAL METHODS Immunodiagnostic Strip Test (Dip Stick Test) Ag Nitrocellulose strip Monoclonal Ab Coloured dye Pt bld (?Ag) +ve -ve Malaria, Filaria, African tryp.
45. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Blood Aspirates PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
46. MOLECULAR BIOLOGICAL TECHNIQUES DNA Probes DNA Probe Commercially prepared DNA sequence Radio active material Nitrocellulose paper Sample (Serum/ stool) ?? parasite +ve parasite Hybridization Radioactivity