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Microbial Diversity: Tapping the Untapped
1.
Bioremediation & Microbial
Diversity: Applications of Molecular Biological Tools in Studying Novel Physiological Traits Suneel Arjun Chhatre Aug 11, 2009
2.
3.
4.
5.
Tapping the Untapped?
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
Isolate DSS 6
: Colony Characteristics
21.
Isolate DSS 8
: Colony Characteristics
22.
Isolate GSS 3
: Colony Characteristics
23.
24.
25.
XylE -Catechol 2-3
Dioxygenase
26.
27.
Gravimetric Analysis of
Various Fractions
28.
29.
Biosurfactant Production by
DSS 6
30.
31.
32.
33.
34.
Strategies to Study
Evolutionary Origin of TCHQ dehalogenase in Sphingobium chlorophenolicum
35.
36.
37.
38.
39.
40.
Insertion of Kanamycin
Gene in the Middle of maai Gene pBS + MAI with Kan (pSS-MK) Kan MAI/2 MAI/1 Overlapping PCR MAI+Kan
41.
42.
PCR to verify
maai gene with insertion of kanamycin resistance gene in mutants With Long primers With short primers M C6 C8 C9 C10 M C6 C8 C9 C10
43.
Confirmation of knockouts
by Phenotype LB+Kan Tyrosine+Minimal Medium
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
Mutations in TCHQ
dehalogenase
57.
Quick-change Mutagenesis (Stratagene)
58.
Sequencing
59.
60.
Results PCR for
2 fragments Overlapping PCR and vector pET 21a Product
61.
Colony PCR and
Sequencing pcpC Blast 2 with pcpC
62.
63.
64.
65.
66.
pH Dependent Protein
Expression in Sulfur Oxidizing Bacteria
67.
68.
69.
70.
71.
72.
“S4”Sulfur Oxidation Pathway
73.
74.
75.
76.
77.
Substrate dependent oxygen
consumption by Halothiobacillus neapolitanus
78.
79.
80.
81.
82.
83.
84.
Purification of Proteins
involved in Sulfur Oxidation
85.
86.
C-554
87.
C-554
88.
89.
90.
91.
92.
93.
94.
CODEHOP ….
95.
CODEHOP Output
96.
97.
Quick-change Mutagenesis (Stratagene)
98.
99.
100.
Creation of Knockout
Mutant by Homologous Recombination Marker pKnock-Km Truncated maai maai from KT 2440 pKnock System
101.
Insertion of Kanamycin
Gene in the Middle of maai Gene pBS + MAI with Kan (pSS-MK) Kan MAI/2 MAI/1 Overlapping PCR MAI+Kan
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
113.
114.
Insertion of Kanamycin
Gene in the Middle of maai Gene pBS + MAI with Kan (pSS-MK) Kan MAI/2 MAI/1 Overlapping PCR MAI+Kan
115.
116.
117.
118.
119.
Mutations in TCHQ
dehalogenase
120.
121.
122.
Purified MAAI on
Glutathione Agarose Elute Wash Flow through Load
123.
Sphingobium chlorophinolicum
grown on Tyrosine Wash Flow through Supernatent Crude Elutions
124.
125.
Colony PCR
M Mutants C
126.
Quick-change Mutagenesis (Stratagene)
127.
Isolate DSS 6
: Colony Characteristics
128.
Isolate DSS 8
: Colony Characteristics
129.
Isolate GSS 3
: Colony Characteristics
130.
131.
132.
133.
134.
Bioremediation of Contaminated
Soil
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