SlideShare uma empresa Scribd logo
1 de 18
Baixar para ler offline
µ-Contact Printing System µCP2.1

short overview of the current status
GeSiM mbH, February 2008
µ-Contact Printing System µCP2.1
                    stamps with PDMS-membrane, scaled in the nm to µm range



                                                                                             compressed air




                           Stamp Chamber             Stampholder                        Stamp Chamber




                                                    Stampframe



                                                   PDMS-Membran

                                                   nm/µm Patterns


  a) Basic Mode: PDMS-membran planar                                b) Print-Mode: PDMS-membran deflected




SEM picture Si-Master   Master in Casting Staion   Casting of PDMS-Stamp        PDMS-Stamp            µCP-Stamping Unit
µ-Contact Printing System µCP2.1
                              stamp in touch with glass slide



                                         PDMS-Stamp               PDMS-Stamp
                                         frame not patterned      10µm lines, 20µm pitch




                  PDMS - Stamp

                      1mm Slide

                 Microscope



a) scheme quot;PDMS-Stamp on Slidequot;          b) photograph shows PDMS-Stamp and slide
                                            perfectly in touch, stamp area (1x1) cm²

Remarks:
The system µCP2.1 guarantees reproducible and perfect contact between the PDMS-
stamp and the substrate.
The USER of µCP2.1 has to develope the surface chemistry to transfere samples, nano-
particles, molecules, bacteria, viruses or cells onto the biochip.
µ-Contact Printing System µCP2.1
                          accessories top view




                                                 Stamp - Washing Station
   µCP-2.1 Accessories



Stamp - Casting Station
µ-Contact Printing System µCP2.1
                           µm- scaled stamps made in PDMS




Fig.1: 10µmx10µm PDMS-mesa structures,       Fig.2: Donut structures, Ø 50µm, a view onto
       a view onto a real stamp surface             a real stamp surface
µ-Contact Printing System µCP2.1
                                                foot print of µCP2.1



 M 1: drive inking                                                     M2: drive stamping


                                                                           M2
     M1                                               N2-blow-dry


                                                                       Stamping- Unit
                     Inking-Station
         Stamp1      Stamp2   Stamp3   Stamp4




                                                     Dry-Station



                      Sample Pad
                                                                         Slide-Tray
                                                                                                 vernier drive
                                                                                                 in y-axis




                                                                                      vernier drive
                                                                                      in x-axis
Top View µ-ContactPrinting System µCP2.1
Dimensions in total LxWxH= (42x40x35)cm³
µ-Contact Printing System µCP2.1
           system housing
µ-Contact Printing System µCP2.1
                         photographs of the real device



                                                               c

                                                b




General View on µCP2.1                              a

                                              • (a) inking station with 4 ink pads
                                              • (b) drying nozzles, two per stamp
                                              • (c) stamping unit
µ-Contact Printing System µCP2.1
                                a video-microscope is implemented from below




                                                                           Stamping Unit




                                                                                           Slide Tray




                                                              Microscope


µCP2.1 mit Videomikroskop                                     X-Y-Slide Tray and Stamp Head
(currently the chassis is under develeopment, the
visible one is a test approach only!)
µ-Contact Printing System µCP2.1
   nano- and micro imprint on the same platform




                                µCP2.1 with an UV-light source
                                The commercial µCP2.1 will be equipped with a manually
                                driven slider, which holds video-microscope and UV-source.
                                As UV-light source we recommend a system from DELO
                                GmbH Germany, for example the system DELOLUX 80.
µ-Imprinting with the System µCP2.1
                                     imprinting of thin polymer films




                                                                         40µm

          50µm



Fig.1: 1,0µm thick polymer film of an UV-active ink,         Fig.2: 1,0µm thick polymer film of an UV-active ink,
coated on a standard glass slide, structure was              coated on a standard glass slide like fig.1,
made by µ-imprinting using a PDMS-stamp, film                lines width 20µm, pitch 40 µm
thickness 2µm, lateral dimensions of the squares
25x25µm², the connecting lines are 2µm wide (Ink
composition: 4-(2-[4-[2-[2-Cyanophenyl)-vinyl]-
phenyl]-vinyl)-benzonitril, diluted with
Tetrahydrofuran)
µ-Imprinting with the System µCP2.1
                           imprinting of spin coated photo restist films




Fig.3: Imprinted photo resist AZ-4562, 8µm thick, on        Fig.4: Imprinted photo resist AZ-1514, 0.5µm
glass slide
PDMS-Stamp: height of structures10µm, lateral               thick on glass slide
dimensions of the squares 25x25µm², the connecting          PDMS-Stamp: like fig.3
lines are 2µm wide                                          Method: like fig.3
Method: spin coating of a high viscous photo resist on a    Remark: in picture 4 a status is shown, where
glass slide, imprinting the PDMS-stamp into the wet         the imprinted resist is not back etched by RIE
resist, drying at RT without UV-radiation on air for 5min
Remark: in pictures 3 a status is shown, where the
imprinted resist is not back etched by RIE

scale bare: 50µm
µ-Contact Printing System µCP2.1
                                     µm- scaled stamps made in PDMS




Fig.5: µ-Contact-Print on hydrophilic     Fig.6: second contact print with the   Fig. 7: third contact print with the same
glass slide using an PDMS-stamp inked     same stamp and method of fig.5         stamp and method of fig.5 without
with a water based CY3-labeled buffer     without nearly inking step             nearly inking step
solution, stamp area 1x1cm², the
stamp pattern consists of thousands of
squares and connecting lines (see the
detail on the right), the stamp gets in
contact with the slide approx. 1 min
after the inking procedure                                         25x25µm²
                                                                   Quadrate
Method: a) inking of PDMS-stamp for
1min, b) drying with compressed air 2
bar/30sec, c) stamp contact at 0,25bar
stamp-pressure/ 60sec. contact time
                                                           2µm Stege
µ-Contact Printing System µCP2.1
                         µ-Contact-Printing and parallel shifts of the substrate


                                           Pattern: A=100




                                   Ø   A
                                 A
                                 Ø
                                 2xA
                                       A
                                 A




                                               2xA




Fig. 8: µ-Contact-Print on hydrophilic glass slide          Fig.9: µ-Contact-Print on hydrophilic glass slide using an
using an PDMS-stamp inked with a water based                PDMS-stamp inked with a water based CY3-labeled buffer
CY3-labeled buffer solution, stamp area 1x1cm²
Method: 2-STEP µ-Contact-Print with parallel shift of       solution, stamp area 1x1cm²
600µm in the y-axis,                                        Method: 2-STEP µ-Contact-Print with parallel shift of
PDMS-stamp: squares and circles are 100µm for               1200µm in the y-axis
parameter A (see detail drawing above)                      PDMS-stamp: squares and circles are 200µm for parameter
Remark: µCP2.1 allows a manually x-y-shift in               A (see detail drawing above)
single steps of 500nm
µ-Contact Printing System µCP2.1
    µ-Contact-Printing to transfer nano-particles




                                       Fig.11: µ-Contact-Printing of spherical gold nano-
                                            particles (Ø=37nm, diluted in water) on a
                                            hydrophilic glass slide, PDMS-Stamp: no O2-
              3µm 8µm                       plasma treatment         stamp-design consists
                                            of thousands of 8x8µm² mesa structures, which
                                            are 10µm high (see the detail in fig.11), the
                                            photograph was created by dark-field upright
                                            microscopy, nano-particle clusters appears in
                                            red

                                       Method: a) inking of the PDMS-stamp, for 60sec
                                           using 40µl of the nano-particle solution, b)
                                           drying of the PDMS-stamp with compressed air
                                           2,0 bar/30sec, c) contact printing, stamp
                                           pressure 1,2 bar /contact time 60 sec
µ-Contact Printing System µCP2.1
                                    µm- scaled stamps made in PDMS




                                                                                        25x25µm²
                                                                                        Quadrate




                                                                                2µm Stege




Fig.12: µ-Contact-Printing of spherical gold nano-particles (Ø=37nm, diluted in water) on a hydrophilic silicon chip, PDMS-
     stamp is not O2-plasma treated, stamp-design consists of thousands 25x25µm² mesa structures connected with 2µm
     wide footbridges, the stamp pattern are 10µm high (see the detail in fig.12), the photograph was created by dark-field
     upright microscopy, nano-particle clusters appears in a light green
Method: a) inking of the PDMS-stamp, for 60sec using 40µl of the nano-particle solution, b) drying of the PDMS-stamp with
     compressed air 2,0 bar/30sec, c) contact printing, stamp pressure 1,2 bar /contact time 60 sec
µ-Contact Printing System µCP2.1
                                                 a first result




Fig.: The glass slide with poly-L-Lysin imprints made       Fig.: The slide after a 19 hours cultivation of L929 cells.
with a PDMS-stamp. The stamp carries donut-mesa             Each donut reacts as an adhesion point for the cells.
patterns wetted before with alginat.


Ref.: M. Gepp, H. Zimmermann - IBMT-St.Ingbert and S. Howitz - GeSiM, Großerkmannsdorf, June 2007
µ-Contact Printing System µCP2.1
  example of a possible nano-structure Silicon-master




                                         Approximated Master Price
                                         4“- Silicon wafer with 10 master chips
                                         containing indically patterns
                                         6.500,- k€

Mais conteúdo relacionado

Semelhante a µCp Description Eng

Modern printing technologies
Modern printing technologiesModern printing technologies
Modern printing technologiesMd Ali Hossain
 
IMI Printhead Selection Raymond 20140903 v1.0
IMI Printhead Selection Raymond 20140903 v1.0IMI Printhead Selection Raymond 20140903 v1.0
IMI Printhead Selection Raymond 20140903 v1.0Michael Raymond
 
Mask fabrication process
Mask fabrication process Mask fabrication process
Mask fabrication process pardis paliz
 
DPL Large scope nano-replication system -www.dpl.dk
DPL Large scope nano-replication system -www.dpl.dkDPL Large scope nano-replication system -www.dpl.dk
DPL Large scope nano-replication system -www.dpl.dkJanny Rasmussen
 

Semelhante a µCp Description Eng (6)

PCB MANUFACTURING
PCB MANUFACTURINGPCB MANUFACTURING
PCB MANUFACTURING
 
Quality control in pigment industry
Quality control in pigment industryQuality control in pigment industry
Quality control in pigment industry
 
Modern printing technologies
Modern printing technologiesModern printing technologies
Modern printing technologies
 
IMI Printhead Selection Raymond 20140903 v1.0
IMI Printhead Selection Raymond 20140903 v1.0IMI Printhead Selection Raymond 20140903 v1.0
IMI Printhead Selection Raymond 20140903 v1.0
 
Mask fabrication process
Mask fabrication process Mask fabrication process
Mask fabrication process
 
DPL Large scope nano-replication system -www.dpl.dk
DPL Large scope nano-replication system -www.dpl.dkDPL Large scope nano-replication system -www.dpl.dk
DPL Large scope nano-replication system -www.dpl.dk
 

Último

Inside Look: Brooke Monk's Exclusive OnlyFans Content Production
Inside Look: Brooke Monk's Exclusive OnlyFans Content ProductionInside Look: Brooke Monk's Exclusive OnlyFans Content Production
Inside Look: Brooke Monk's Exclusive OnlyFans Content Productionget joys
 
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ..."Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...RAGHURAMYC
 
Carowinds 2024: Thrills, Spills & Surprises
Carowinds 2024: Thrills, Spills & SurprisesCarowinds 2024: Thrills, Spills & Surprises
Carowinds 2024: Thrills, Spills & Surprisescarawinds99
 
Holi:: "The Festival of Colors in India"
Holi:: "The Festival of Colors in India"Holi:: "The Festival of Colors in India"
Holi:: "The Festival of Colors in India"IdolsArts
 
Taylor Swift quiz( with answers) by SJU quizzers
Taylor Swift quiz( with answers) by SJU quizzersTaylor Swift quiz( with answers) by SJU quizzers
Taylor Swift quiz( with answers) by SJU quizzersSJU Quizzers
 
Young adult book quiz by SJU quizzers.ppt
Young adult book quiz by SJU quizzers.pptYoung adult book quiz by SJU quizzers.ppt
Young adult book quiz by SJU quizzers.pptSJU Quizzers
 
5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life
5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life
5 Moments of Everyday Self-Loathing That Perfectly Describe Your LifeSalty Vixen Stories & More
 

Último (7)

Inside Look: Brooke Monk's Exclusive OnlyFans Content Production
Inside Look: Brooke Monk's Exclusive OnlyFans Content ProductionInside Look: Brooke Monk's Exclusive OnlyFans Content Production
Inside Look: Brooke Monk's Exclusive OnlyFans Content Production
 
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ..."Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...
"Quest for Knowledge: An Exciting Journey Through 40 Brain-Bending Questions ...
 
Carowinds 2024: Thrills, Spills & Surprises
Carowinds 2024: Thrills, Spills & SurprisesCarowinds 2024: Thrills, Spills & Surprises
Carowinds 2024: Thrills, Spills & Surprises
 
Holi:: "The Festival of Colors in India"
Holi:: "The Festival of Colors in India"Holi:: "The Festival of Colors in India"
Holi:: "The Festival of Colors in India"
 
Taylor Swift quiz( with answers) by SJU quizzers
Taylor Swift quiz( with answers) by SJU quizzersTaylor Swift quiz( with answers) by SJU quizzers
Taylor Swift quiz( with answers) by SJU quizzers
 
Young adult book quiz by SJU quizzers.ppt
Young adult book quiz by SJU quizzers.pptYoung adult book quiz by SJU quizzers.ppt
Young adult book quiz by SJU quizzers.ppt
 
5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life
5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life
5 Moments of Everyday Self-Loathing That Perfectly Describe Your Life
 

µCp Description Eng

  • 1. µ-Contact Printing System µCP2.1 short overview of the current status GeSiM mbH, February 2008
  • 2. µ-Contact Printing System µCP2.1 stamps with PDMS-membrane, scaled in the nm to µm range compressed air Stamp Chamber Stampholder Stamp Chamber Stampframe PDMS-Membran nm/µm Patterns a) Basic Mode: PDMS-membran planar b) Print-Mode: PDMS-membran deflected SEM picture Si-Master Master in Casting Staion Casting of PDMS-Stamp PDMS-Stamp µCP-Stamping Unit
  • 3. µ-Contact Printing System µCP2.1 stamp in touch with glass slide PDMS-Stamp PDMS-Stamp frame not patterned 10µm lines, 20µm pitch PDMS - Stamp 1mm Slide Microscope a) scheme quot;PDMS-Stamp on Slidequot; b) photograph shows PDMS-Stamp and slide perfectly in touch, stamp area (1x1) cm² Remarks: The system µCP2.1 guarantees reproducible and perfect contact between the PDMS- stamp and the substrate. The USER of µCP2.1 has to develope the surface chemistry to transfere samples, nano- particles, molecules, bacteria, viruses or cells onto the biochip.
  • 4. µ-Contact Printing System µCP2.1 accessories top view Stamp - Washing Station µCP-2.1 Accessories Stamp - Casting Station
  • 5. µ-Contact Printing System µCP2.1 µm- scaled stamps made in PDMS Fig.1: 10µmx10µm PDMS-mesa structures, Fig.2: Donut structures, Ø 50µm, a view onto a view onto a real stamp surface a real stamp surface
  • 6. µ-Contact Printing System µCP2.1 foot print of µCP2.1 M 1: drive inking M2: drive stamping M2 M1 N2-blow-dry Stamping- Unit Inking-Station Stamp1 Stamp2 Stamp3 Stamp4 Dry-Station Sample Pad Slide-Tray vernier drive in y-axis vernier drive in x-axis Top View µ-ContactPrinting System µCP2.1 Dimensions in total LxWxH= (42x40x35)cm³
  • 7. µ-Contact Printing System µCP2.1 system housing
  • 8. µ-Contact Printing System µCP2.1 photographs of the real device c b General View on µCP2.1 a • (a) inking station with 4 ink pads • (b) drying nozzles, two per stamp • (c) stamping unit
  • 9. µ-Contact Printing System µCP2.1 a video-microscope is implemented from below Stamping Unit Slide Tray Microscope µCP2.1 mit Videomikroskop X-Y-Slide Tray and Stamp Head (currently the chassis is under develeopment, the visible one is a test approach only!)
  • 10. µ-Contact Printing System µCP2.1 nano- and micro imprint on the same platform µCP2.1 with an UV-light source The commercial µCP2.1 will be equipped with a manually driven slider, which holds video-microscope and UV-source. As UV-light source we recommend a system from DELO GmbH Germany, for example the system DELOLUX 80.
  • 11. µ-Imprinting with the System µCP2.1 imprinting of thin polymer films 40µm 50µm Fig.1: 1,0µm thick polymer film of an UV-active ink, Fig.2: 1,0µm thick polymer film of an UV-active ink, coated on a standard glass slide, structure was coated on a standard glass slide like fig.1, made by µ-imprinting using a PDMS-stamp, film lines width 20µm, pitch 40 µm thickness 2µm, lateral dimensions of the squares 25x25µm², the connecting lines are 2µm wide (Ink composition: 4-(2-[4-[2-[2-Cyanophenyl)-vinyl]- phenyl]-vinyl)-benzonitril, diluted with Tetrahydrofuran)
  • 12. µ-Imprinting with the System µCP2.1 imprinting of spin coated photo restist films Fig.3: Imprinted photo resist AZ-4562, 8µm thick, on Fig.4: Imprinted photo resist AZ-1514, 0.5µm glass slide PDMS-Stamp: height of structures10µm, lateral thick on glass slide dimensions of the squares 25x25µm², the connecting PDMS-Stamp: like fig.3 lines are 2µm wide Method: like fig.3 Method: spin coating of a high viscous photo resist on a Remark: in picture 4 a status is shown, where glass slide, imprinting the PDMS-stamp into the wet the imprinted resist is not back etched by RIE resist, drying at RT without UV-radiation on air for 5min Remark: in pictures 3 a status is shown, where the imprinted resist is not back etched by RIE scale bare: 50µm
  • 13. µ-Contact Printing System µCP2.1 µm- scaled stamps made in PDMS Fig.5: µ-Contact-Print on hydrophilic Fig.6: second contact print with the Fig. 7: third contact print with the same glass slide using an PDMS-stamp inked same stamp and method of fig.5 stamp and method of fig.5 without with a water based CY3-labeled buffer without nearly inking step nearly inking step solution, stamp area 1x1cm², the stamp pattern consists of thousands of squares and connecting lines (see the detail on the right), the stamp gets in contact with the slide approx. 1 min after the inking procedure 25x25µm² Quadrate Method: a) inking of PDMS-stamp for 1min, b) drying with compressed air 2 bar/30sec, c) stamp contact at 0,25bar stamp-pressure/ 60sec. contact time 2µm Stege
  • 14. µ-Contact Printing System µCP2.1 µ-Contact-Printing and parallel shifts of the substrate Pattern: A=100 Ø A A Ø 2xA A A 2xA Fig. 8: µ-Contact-Print on hydrophilic glass slide Fig.9: µ-Contact-Print on hydrophilic glass slide using an using an PDMS-stamp inked with a water based PDMS-stamp inked with a water based CY3-labeled buffer CY3-labeled buffer solution, stamp area 1x1cm² Method: 2-STEP µ-Contact-Print with parallel shift of solution, stamp area 1x1cm² 600µm in the y-axis, Method: 2-STEP µ-Contact-Print with parallel shift of PDMS-stamp: squares and circles are 100µm for 1200µm in the y-axis parameter A (see detail drawing above) PDMS-stamp: squares and circles are 200µm for parameter Remark: µCP2.1 allows a manually x-y-shift in A (see detail drawing above) single steps of 500nm
  • 15. µ-Contact Printing System µCP2.1 µ-Contact-Printing to transfer nano-particles Fig.11: µ-Contact-Printing of spherical gold nano- particles (Ø=37nm, diluted in water) on a hydrophilic glass slide, PDMS-Stamp: no O2- 3µm 8µm plasma treatment stamp-design consists of thousands of 8x8µm² mesa structures, which are 10µm high (see the detail in fig.11), the photograph was created by dark-field upright microscopy, nano-particle clusters appears in red Method: a) inking of the PDMS-stamp, for 60sec using 40µl of the nano-particle solution, b) drying of the PDMS-stamp with compressed air 2,0 bar/30sec, c) contact printing, stamp pressure 1,2 bar /contact time 60 sec
  • 16. µ-Contact Printing System µCP2.1 µm- scaled stamps made in PDMS 25x25µm² Quadrate 2µm Stege Fig.12: µ-Contact-Printing of spherical gold nano-particles (Ø=37nm, diluted in water) on a hydrophilic silicon chip, PDMS- stamp is not O2-plasma treated, stamp-design consists of thousands 25x25µm² mesa structures connected with 2µm wide footbridges, the stamp pattern are 10µm high (see the detail in fig.12), the photograph was created by dark-field upright microscopy, nano-particle clusters appears in a light green Method: a) inking of the PDMS-stamp, for 60sec using 40µl of the nano-particle solution, b) drying of the PDMS-stamp with compressed air 2,0 bar/30sec, c) contact printing, stamp pressure 1,2 bar /contact time 60 sec
  • 17. µ-Contact Printing System µCP2.1 a first result Fig.: The glass slide with poly-L-Lysin imprints made Fig.: The slide after a 19 hours cultivation of L929 cells. with a PDMS-stamp. The stamp carries donut-mesa Each donut reacts as an adhesion point for the cells. patterns wetted before with alginat. Ref.: M. Gepp, H. Zimmermann - IBMT-St.Ingbert and S. Howitz - GeSiM, Großerkmannsdorf, June 2007
  • 18. µ-Contact Printing System µCP2.1 example of a possible nano-structure Silicon-master Approximated Master Price 4“- Silicon wafer with 10 master chips containing indically patterns 6.500,- k€