What's New in Teams Calling, Meetings and Devices March 2024
Prioritizing Scaffolds for Hit Selection in High Throughput Screening Programs
1. Priori%zing Scaffolds for Hit Selec%on
in High Throughput Screening
Programs
Rajarshi Guha, Dac‐Trung Nguyen
NIH Chemical Genomics Center
6th Indo‐US Workshop on Mathema%cal Chemistry
8th – 10th January, 2010
3. NIH Chemical Genomics Center
Assay development Compound
and op2miza2on Op2miza2on
Small Molecules
Biology Chemistry
NCGC
Informa%cs ACOM
Genome wide RNAi
SAR analysis, method & Automa2on, Compound
tool development management
4. High Throughput Screening
• Assay thousands to
hundreds of thousands
of compounds
– Biochemical, gene%c,
pharmacological assays
• Rapidly iden%fy novel
modulators of biological
systems
• Lots of raw data –
automated analy%cs are
essen%al
5. Hun%ng for Leads
Target Lead Lead Clinical
Iden%fica%on Discovery Op%miza%on Development
HTS
Primary
Confirma%on
• Sensi%vity Screening • Select subset
• Scaling • Fluorescence to follow up • Counter
• High Content • Diversity screen
• Explore SAR
Assay
Cherry Picking
Op%miza%on