Worm timing paper

•

0 likes•1,475 views

Arjun Raj

Health & Medicine Technology

Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate speciﬁcation!
Gautham
Nair,
Travis
Walton,
John
Isaac
Murray,
and
Arjun
Raj

University
of
Pennsylvania
Department
of
Bioengineering

University
of
Pennsylvania
Department
of
GeneDcs

Transcription
is slowed
THE SAME
- many other genes
- clk-1 mutant slowdown

Transcription
is slowed
THE SAME
Total Number of Cells in Embryo

Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?

emb-29(g52)
mutant
Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?
NO

Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway

Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate speciﬁcation!

Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate speciﬁcation!
Funding:

HHMI
Life
Sciences
Research
FoundaDon
Fellowship

Burroughs-‐Wellcome
Fund

NIH
New
Innovator
Award

Penn
Genome
FronDers
InsDtute

Pennsylvania
Department
of
Health

What's hot

Genome rearrangment

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Sanjay Kr. Vishwakarma

GENECTIC REGULATION OF DEVELOPMENT IN DROSOPHILA. BY, MAYURA D. JOSHI K. J. SOMAIYA COLLEGE OF SCIENCE AND COMMERCE. MSC--PART II--BOTANY PAPER--VI HIERARCHY OF GENE ACTIVATION. MATERNAL GENES. SEGMENTATION GENES OF EMBRYO. HOMEOTIC GENES OF EMBRYO. HOMEOTIC SELECTOR GENES. MUTATIONS IN HOMEOTIC GENES. OTHER GENES OF THE EMBRYO. SEX DETERMINATION IN DROSOPHILA. THE HOMEOTIC SELECTOR GENES OF EMBRYO. Once the segmentation pattern has been determined, a major class of genes called the homeotic (structure-determining) selector genes are activated. This genes specify the identity of each segment including the body part that will develop at metamorphosis. Means this genes are master regulatory genes which specify the types of appendages and other structures that each segment will form. Mutations in homeotic genes produce flies with structures in incorrect places. Homeotic genes control the expression of genes responsible for specific anatomical structures. E.g. Where antenna should form, where legs should appear etc. Mutations in Homeotic genes. The homeotic genes have been defined by mutations that affect the development of the fly. That is, homeotic mutations alter the identify of particular segments, transforming them into copies of other segments. The principal pioneer of genetic studies of homeotic mutations is Edward Lewis. More recent molecular analysis has been done in many laboratories, including those of Thomas Kaufman, Walter Gehring, William McGinnis, Matthew Scott, and Welcome Bender. Lewis’s pioneering studies were on a cluster of homeotic genes called the bithorax complex (BX-C). BX-C determines the posterior identity of the fly, namely, thoracic segment T3 and abdominal segments AI-A8. BX-C contains three genes called Ultrabithorax (Ubx), Abdominal-A (Abd-A), and Abdominal-B (Abd-B). Each of these genes constitutes of one protein-coding transcription unit. Mutations in these homeotic genes often are lethal and the fly typically does not survive. Some nonlethal mutant alleles have been characterized. These nonlethal mutant alleles allow an adult fly to develop. The figure given here, shows the abnormal adult structures that can result from bithorax mutations. A diagram showing the segments of a normal adult fly in the Figure (a); note that the wings are located on segment Thorax 2 (T2). The pair of halteres (rudimentary wings used as balancers in flight) are on segment T3. A photograph of a normal adult fly clearly showing the wings and halteres is presented in Figure (b). Figure (c); shows one type of developmental abnormality that can result from nonlethal homeotic mutations in BX-C.

Bot msc part ii sem 4_2014-15_paper 6_mayura d. joshi

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http://gagueira.wordpress.com Mutações homozigóticas nos genes GNPTAB e GNPTG estão classicamente associadas às mucolipidoses tipo II (ML II, alfa/beta) e tipo III (ML III, alfa/beta/gama), que são doenças raras de armazenamento lisossômico caracterizadas por múltiplas patologias. Recentemente, variantes nos genes GNPTAB, GNPTG e NAGPA (este último funcionalmente relacionado aos dois primeiros) foram associadas com a gagueira persistente não-sindrômica. Analisando uma amostra de escala mundial que abrangia 1.013 indivíduos com gagueira persistente não-sindrômica, sem relação de parentesco, encontramos 164 indivíduos que carregavam alguma rara variante não-sinônima de codificação em um destes três genes. Comparamos a frequência destas variantes com aquelas encontradas em grupos-controle nas mesmas populações e também em bancos de dados genômicos, e confrontamos a localização dessas mutações com a localização das mutações relatadas nas mucolipidoses. Descobrimos que pessoas com gagueira exibiam um excesso de variantes não-sinônimas de codificação quando comparadas a pessoas dos grupos-controle e a genomas pertencentes às bases de dados dos projetos “1000 Genomes” e “Exome Sequencing”. Ao todo, foram identificadas 81 variantes diferentes nos casos de gagueira analisados pelo nosso estudo. Praticamente todas eram substituições do tipo missense, das quais apenas uma tinha sido previamente relatada na mucolipidose, uma doença geralmente associada a mutações mais amplas, que levam a uma perda completa de função da proteína codificada. Nossa conclusão é que essas raras variantes de codificação não-sinônimas nos genes GNPTAB, GNPTG, e NAGPA podem ser responsáveis por até 16% dos casos de gagueira persistente, e que as alterações nos genes GNPTAB e GNPTG ocorrem em posições da cadeia nucleotídica que, em geral, causam efeitos menos graves na função da proteína do que aqueles vistos nas mucolipidoses tipo II e III.

Mucolipidoses Tipo II e III e Gagueira Não-Sindrômica são condições genéticas...

Stuttering Media

Cell authentication by str profile

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leu- bacteria are mixest in a flask with and leut bacteria, and soon all bacteria are leut. However, if the leu cells are on one side of a U-tube and the leu + cells are on the other, the leu cells do not become prototrophic. This suggests which mechanism of gene transfer? specialized transduction conjugation transformation generalized transduction When mapping the relative positions of three linked genes by examining traits of progeny in a test cross, changes in phenotype due to movement of the allele in the middle of the three, relative to the parental alleles, will occur movement of the flanking alleles. more frequently than lehs frequently than with approximately the same frequency as What was demonstrated by experiments conducted by Heinz Fraenkel- Conrat and Singer? DNA is replicated semi-conservatively. DNA was the genetic component of bacterial cells. DNA was the genetic component of T 1 viruses. RNA is the genetic component of a plant virus. are most likely to cause genes to move from one linkage group to another. Unequal crossing over Deletions Inversions Translocations Which bond is not found in the sugars that make up DNA? 2 C OH 5'C-phosphate 1'C-nitrogenous base 3'C-OH If a DNA molecule of 50 base pairs in length contains 15 cytosine bases, how many thymine bases will it have? 30 15 35 10.

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maryamsarwar17

Comparative sequence studies of the repeat elements in diverse insect species can provide useful information on how to make use of them for developing abundant markers that can be used in those species; $ At the moment, a total of 8 species are in genome assembly stages and another 35 are in progress for genome sequencing; $ Different molecular marker systems in the field of entomology are expected to provide new directions to study insect genomes in an unprecedented way in the years to come

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