We primarily use popping buffer and PBS for DNA-unzipping and overstretching experiments. All the other buffers consists these two.

1. Buffers used in DNA-overstretching
and unzipping experiments

2. We primarily use popping buffer and PBS for DNA-unzipping
and overstretching experiments
• Popping Buffer
• PBS

3. Popping Buffer
Chemicals used in popping buffer:
• EDTA:

4. • Sodium Phosphate
• Monobasic:

5. • Dibasic

6. • NaCl

7. • Tween20:

8. • Water H2O:

9. • D2O

10. Quantities and measurements

11. Calculations
LabView

12. Weight & Measure

13. Store

14. PBS
Chemicals used in PBS: PBS and H2O or D2O
• Dissolve 1 table in
200mL of H2O or D2O

15. BGB
Blotting Grade Blocker
To make 5mg/ml BGB
solution in popping
buffer:
•First wiight out 15mg of
BGB poweder
•Add 1.5 ml of 1x pop
and mix by vortexing
•Run through .2μM
syringe filter using 3ml
syringe.
•Now store 5mg/ml BGB
in 1x pop buffer (minus
amount of protein that
stuck to filter) at 3C.
This should be good for
few weeks.

16. Filter

17. Anti-Dig

18. Microspheres

19. DNA